MA Wenyu,YANG Chengxi,GUO Weiqiang,et al.Effects of Rhus typhina on the Soil Microbial Community Functional Diversity of the Cosmos bipinnata Rhizosphere[J].Northern Horticulture,2019,43(21):64-71.[doi:10.11937/bfyy.20191256]
火炬树对波斯菊根际土壤微生物群落功能多样性的影响
- Title:
- Effects of Rhus typhina on the Soil Microbial Community Functional Diversity of the Cosmos bipinnata Rhizosphere
- 关键词:
- 火炬树; 波斯菊; Biolog-ECO; 碳源利用; 土壤微生物功能多样性
- Keywords:
- Rhus typhina; Cosmos bipinnatus; Biolog-ECO; carbon source utilization; soil microbial functional diversity
- 文献标志码:
- A
- 摘要:
- 以火炬树（Rhus typhina）根为试验材料，波斯菊（Cosmos bipinnatus）为研究对象，采用室内盆栽模拟控制试验，研究不同浓度火炬树根水浸提液对波斯菊生长指标的影响；用Biolog-ECO微平板培养法和土壤理化性质常规测定法，研究土壤微生物碳源利用特性，通过冗余分析（RDA）研究影响土壤微生物碳源利用的主要因素及作用机理。通过研究波斯菊根际土壤微生物功能多样性及其对火炬树不同浓度根水浸提液的响应，探讨火炬树对波斯菊化感作用机理。结果表明：TB条件下波斯菊的生长状况最好；不同处理对土壤pH、电导率、总氮含量、有机碳含量、碳氮比的影响显著（P<0.05）；不同浓度处理下，波斯菊土壤微生物单孔颜色平均变化率（AWCD）均随着培养时间的增加逐渐上升，并在180 h时AWCD达到最大值并趋于稳定，不同处理间依次为TB>TA>TC>CK>TD；各根水浸提液处理均促进了土壤微生物对胺类物质的利用，抑制了对氨基酸类和多聚物类物质的利用；不同处理对波斯菊土壤微生物Shannon-Wiener多样性指数、Pielou均匀度指数影响均存在显著差异（P<0.05），Gini多样性指数差异不显著（P>0.05）。RDA分析表明，总氮和有机碳是影响土壤微生物碳源利用的主要因素。火炬树根水浸提液可以影响土壤微生物群落功能多样性，进一步改变土壤理化性质，从而影响波斯菊的生长。
- Abstract:
- The roots of Rhus typhina were used as experimental material and the Cosmos bipinnatus was used as research object.They were selected in the basin to measure plant growth indicators.Then the carbon source utilization characteristics of soil microbial community under C.bipinnatus was detected by Biolog-ECO micro-plate and soil physicochemical properties were measured by conventional methods under different concentrations aqueous extracts of roots.Meanwhile redundancy analysis (RDA) were used to study the main factors affecting the carbon source utilization of soil microorganisms and the mechanism of action.The aim of the present study was to investigate the soil microbial functional diversity of Cosmos bipinnatus rhizosphere and its response to different concentrations root aqueous extract of Rhus typhina，and to explore the allelopathic mechanism of R.typhina to C.bipinnatus.The results showed that C.bipinnatus was the best under TB treatment.There was significant difference in soil pH，total nitrogen content，carbon nitrogen ratio，electrical conductivity and organic carbon content (P<0.05).Under different treatments，the average well color development (AWCD) gradually increased with the increasing culture time，and the maximum value was at 180 hours，and the order of carbon source utilization was as follow：TB，TA，TC，CK，TD.Compared with the CK，other treatments promoted the utilization of amines by soil microorganisms and inhibited the utilization of amino acids and polymers.There were significant differences in Shannon-Wiener diversity index，Pielou uniformity index was not (P<0.05),but Gini diversity index (P>0.05) under different treatments.RDA showed that total nitrogen content and organic carbon content was the main factors affecting the carbon sources utilization of soil microbes.The aqueous extracts of R.typhina roots could further change the functional diversity of soil microbial community by affecting soil physical and chemical properties.Thus it affect the growth of the plant.
参考文献/References:
[1]林先贵.土壤微生物研究原理与方法[M].北京:高等教育出版社,2010.[2]王轶.长期施肥对温室和大田土壤微生物的影响[D].北京:中国农业大学,2014.[3]BEEST M,STEVEN N,OLFF H,et al.Plant-soil feedback induces shifts in biomass allocation the invasive plant Chromolaenaodorat[J].Journal of Ecology,2009(6):1281-1290.[4]JORDAN N R,LARSON D L,HUERD S C.Soil modification by invasive plant:Effects on native and invasive species of mixed-grass prairies[J].Biological Invasions,2008,10:177-190.[5]廖亚丽.入侵植物加拿大一枝黄花凋落叶的化感作用及机理研究[D].镇江:江苏大学,2017.[6]杨琼,梁羽,杨剑,等.入侵植物薇甘菊的根际土壤微生物特征[J].生态科学,2015,34(2):148-155.[7]HAWKES C V,WREN I F,HERMAN D J,et al.Plant invasion alters nitrogen cycling by modifying the soil nitrifying community[J].Ecology Letters,2005,8(9):976-985.[8]喻晓丽,蔡体久,宋丽萍,等.火炬树对水分胁迫的生理生化反应[J].东北林业大学学报,2007,35(6):10-12.[9]喻晓丽,邸雪颖,宋丽萍.水分胁迫对火炬树幼苗生长和生理特性的影响[J].林业科学,2007,43(11):57-61.[10]段艳芳,赵辉,樊巍.太行山南端外来树种火炬树群落的水保效应[J].中国农学通报,2012,28(25):43-46.[11]刘永丽,赵国华,王广海,等.火炬树扩散风险性分析[J].河北林业科技,2010(1):43-46.[12]黄乔乔,许慧,范志伟,等.火炬树入侵黑松幼林过程中对土壤化学性质的影响[J].生态环境学报,2013,22(7):1119-1123.[13]樊巍,高喜荣,赵东,等.太行山退化山地火炬树群落物种多样性与土壤特性变化的研究[J].河南农业大学学报,2008,42(3):299-302.[14]侯玉平,柳林,王信,等.外来植物火炬树水浸液对土壤微生态系统的化感作用[J].生态学报,2013,33(13):4041-4049.[15]卜庆梅,侯玉平,房洪坤,等.外来树种火炬树入侵的生理生态特性:与同属本地种盐肤木的比较[J].林业科学,2017,53(5):1-7.[16]张恒庆,穆晓红,贾鑫,等.大连地区外来物种火炬树的调查研究[J].吉林师范大学学报(自然科学版),2018,39(2):104-107.[17]曹永昌,杨瑞,刘帅,等.秦岭典型林分夏秋两季根际与非根际土壤微生物群落结构[J].生态学报,2017,37(5):1667-1676.[18]鲁顺保,张艳杰,陈成榕,等.基于BIOLOG指纹解析三种不同森林类型土壤细菌群落功能差异[J].土壤学报,2013,50(3):618-623.[19]蔡艳,郝明德,臧逸飞,等.不同轮作制下长期施肥旱地土壤微生物多样性特征[J].核农学报,2015,29(2):344-350.[20]申卫收,林先贵,张华勇,等.不同施肥处理下蔬菜塑料大棚土壤微生物活性及功能多样性[J].生态学报,2008,28(6):2682-2689.[21]陈学林,张丽娜,王桔红,等.红毛草入侵程度对根际土壤微生物的影响[J],西北农林科技大学学报(自然科学版),2017,45(4):165-172.[22]卞方圆,钟哲科,张小平,等.毛竹和伴矿景天对重金属污染土壤的修复作用和对微生物群落的影响[J].林业科学,2018,54(8):106-119.[23]宋收,陈晓明,肖伟,等.基于BIOLOG指纹解析土壤可培微生物对铀污染的响应[J].核农学报,2016,30(6):1169-1177.[24]房君佳,李强,刘畅,等.铅锌尾矿砂污染下的岩溶土壤微生物群落碳源代谢特征[J].环境科学,2018,39(5):2420-2430.[25]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000.[26]毛美琴,赵燕,魏玉兰,等.滇重楼根水浸液对四种园林植物的化感作用研究[J].浙江农业学报,2018,30(1):80-90.[27]李雪枫,王坚,许文博,等.冷蒿对三种禾本科植物种子萌发和幼苗生长的化感作用[J].应用生态学报,2010,21(7):1702-1708.[28]滕嘉玲,贾荣亮,赵芸.沙埋对干旱沙区真藓结皮层细菌群落结构和多样性的影响[J].生态学报,2017,37(7):2179-2187.[29]NIU H B,LIU W X,WAN F H,et al.An invasive aster (Ageratina adenophora) invades and dominates forest understories in China:Altered soil microbial communities facilitate the invader and inhibit natives[J].Plant and Soil,2007,294:73-85.[30]TINSINA B,SHRESTHA B B,ROKAYA M B,et al.Impact of Parthenium hysterophorus L.invasion on plant species composition and soil properties of grassland communities in Nepal[J].Flora,2011,206:233-240.[31]张海霞.不同入侵植物对本土植物根际土壤酶活性及微生物数量的影响[J].广东农业科学,2014,41(21):61-66.[32]全国明,毛丹鹃,章家恩,等.五爪金龙、南美蟛蜞菊入侵对土壤化学和微生物学性质的影响[J].植物营养与肥料学报,2016,22(2):437-449.[33]JANDOV K,KLINEROV T,MLLEROV J,et al.Long-term impact of Heracleum mantegazzianum invasion on soil chemical and biological characteristics[J].Soil Biology and Biochemistry,2014,68:270-278.[34]KONOPKA A,OLIVER L,TURCO R F.The use of carbon substrate utilization patterns in environmental and ecological microbiology[J].Microbial Ecology,1998,35:103-115.[35]孙雪,韩冬雪,刘岩,等.原始红松林土壤理化及微生物碳代谢特征对生长季动态的响应[J].南京林业大学学报(自然科学版),2017,41(5):18-26.[36]徐秋芳,姜培坤,邬奇峰,等.集约经营板栗林土壤微生物量碳与微生物多样性研究[J].林业科学,2007,43(3):15-19.[37]张静,温仲明,李鸣雷,等.外来物种刺槐对土壤微生物功能多样性的影响[J].生态学报,2018,38(14):4964-4974.[38]赵艳云,胡相明,刘京涛.贝壳堤地区微生物分布特征及其与植被分布的关系[J].水土保持通报,2012,32(2):267-270.[39]高雪峰,韩国栋,张国刚.短花针茅荒漠草原土壤微生物群落组成及结构[J].生态学报,2017,37(15):1-8.[40]KLOSE S,ACOSTA-MARTNEZ V,AJWA H A.Microbial community composition and enzyme activities in a sandy loam soil after fumigation with methyl bromide or alternative biocides[J].Soil Biology and Biochemistry,2006,38(6):1243-1254.[41]刘秉儒,张秀珍,胡天华,等.贺兰山不同海拔典型植被带土壤微生物多样性[J].生态学报,2013,33(22):7211-7220.[42]KOWALCHUK G A,BUMA D S,de BOER W,et al.Effects of above-ground plant species composition and diversity on the diversity of soil-borne microorganisms[J].Antonie van Leeuwenhoek,2002,81(1/4):509-520.[43]肖鸿光.两种外来植物共同入侵对土壤微生物群落结构的复合影响[D].镇江:江苏大学,2016.[44]ZHENG H,CHEN F L,OUANG Z Y,et al.Utilization of different carbon sources types in biolog-GN microplates by soil microbial communities from four forest types[J].Environmental Science,2007,28(5):1126-1130.
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备注/Memo
第一作者简介：马文育（1991-），女，硕士研究生，研究方向为园林植物应用与景观生态。E-mail：1505988217@qq.com.责任作者：曲同宝（1970-），男，博士，副教授，硕士生导师，现主要从事植物学与植物生态学及园林植物等研究工作。E-mail：qvtb@sina.com.基金项目：国家自然科学基金重点资助项目（31230012）；吉林省科技厅资助项目（20190303078SF）。收稿日期：2019-07-12