[1]张翠芳,王世伟,马彬,等.‘新新2号’核桃果实发育进程中碳水化合物积累与变化[J].北方园艺,2019,43(21):13-19.[doi:10.11937/bfyy.20190599]
　ZHANG Cuifang,WANG Shiwei,MA Bin,et al.Carbohydrate Accumulation and Change During the Development of Juglans.regia ‘Xinxin2’ Fruit[J].Northern Horticulture,2019,43(21):13-19.[doi:10.11937/bfyy.20190599]
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‘新新2号’核桃果实发育进程中碳水化合物积累与变化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年21 页码: 13-19 栏目: 出版日期: 2019-11-15
Title:
Carbohydrate Accumulation and Change During the Development of Juglans.regia ‘Xinxin2’ Fruit
作者:
张翠芳; 王世伟; 马彬; 吉昱; 张梅; 马海燕
（新疆农业大学 林学与园艺学院，新疆教育厅干旱区林业生态与产业技术重点实验室，新疆 乌鲁木齐 830052）
Author(s):
ZHANG Cuifang; WANG Shiwei; MA Bin; JI Yu; ZHANG Mei; MA Haiyan
(College of Forestry and Horticulture，Xinjiang Agricultural University/Key Laboratory of Forestry Ecology and Industry Technology in Arid Region，Education Department of Xinjiang，Urumqi,Xinjiang 830052)
关键词:
核桃; 青皮; 种仁; 碳水化合物; 酶
Keywords:
walnut; green peel; kernel; carbohydrate; enzyme
DOI:
10.11937/bfyy.20190599
文献标志码:
A
摘要:
以新疆南疆盆地核桃主栽品种‘新新2号’（Juglans regia ‘Xinxin2’）为研究对象，研究果实不同生长发育时节其青皮和种仁中葡萄糖、蔗糖、果糖、淀粉含量和中性转化酶（NI）、酸性转化酶（AI）、蔗糖合成酶（SS）、蔗糖磷酸合成酶（SPS）的活性变化。结果表明：花后45～70 d，果实处于速生生长期，青皮中果糖和葡萄糖含量高于蔗糖和淀粉，AI活性保持较高水平，种仁中果糖含量高于蔗糖、葡萄糖和淀粉含量；花后90～110 d，果实处于油脂转化期，青皮蔗糖含量和SS、SPS活性迅速升高，种仁蔗糖和葡萄糖含量高于果糖含量，SS和SPS活性急剧升高，NI和AI活性保持较低水平。相关分析结果显示，青皮蔗糖含量与SS活性、SPS活性呈显著正相关（P＜0.05），果糖含量与AI活性呈显著正相关（P＜0.05）；种仁淀粉含量与青皮淀粉含量呈显著正相关（P＜0.05），与青皮AI活性呈显著负相关（P＜0.05）。在速生生长期，核桃果实青皮中的蔗糖在高活性的AI作用下被分解成葡萄糖和果糖，为果实细胞分裂和形态构建提供物质和能量基础；在油脂转化期，核桃果实青皮和种仁中蔗糖的积累是高活性的SS、SPS和低活性的AI、NI共同作用的结果。
Abstract:
The main walnut cultivar ‘Xinxin2’ (Juglans.regia) in Southern Xinjiang Basin was used as the research object to study the the changes in contents of glucose，sucrose，fructose，starch and the activities of neutral invertase (NI)，acid invertase (AI)，sucrose synthase (SS) and sucrose phosphate synthase (SPS) in green peel and kernels sampled at different growth stages.The results showed that 45-70 days after flowering (DAF)，the fruit was in the fast-growing period；in green peel，the contents of fructose and glucose were higher than those of sucrose and starch，and AI activity remained high；in kernels，the fructose content was higher than those of sucrose，glucose and starch.90-110 DAF，the fruit was in the stage of oil transformation；in green peel，the sucrose content and the activities of SS and SPS increased rapidly；in kernels，the contents of sucrose and glucose were higher than that of fructose，and the activities of SS and SPS increased sharply，while the activities of NI and AI remained low.The results of correlation analysis showed that sucrose content in green peel was positively correlated with the activities of SS and SPS (P<0.05)，fructose content was positively correlated with AI activity (P<0.05)，starch content in seed kernel was positively correlated with starch content in green peel (P<0.05)，and negatively correlated with AI activity in green peel (P<0.05).During fast-growing period，sucrose in walnut green peel was decomposed into glucose and fructose under the action of high-activity AI，which provided material and energy basis for cell division and morphological construction of walnut.During oil transformation period，sucrose accumulation in walnut green peel and kernel was the result of high-activity SS，SPS and low-activity AI and NI.

参考文献/References:

[1]许大全.光合作用学[M].北京:科学出版社,2013.[2]潘瑞炽.植物生理学[M].4版.北京:高等教育出版社,2001.[3]石斌.不同库源关系对油茶光合作用及同化物分配的影响[D].长沙:中南林业科技大学,2015.[4]李卫东.桃库源关系中源叶光合作用及其碳水化合物代谢的研究[D].北京:中国农业大学,2005.[5]郗荣庭.中国核桃（Juglans regia L.）起源考证[J].河北农业大学学报,1990,13(1):90-94.[6]王磊,崔乃然,张汉斐.新疆野生核桃的研究[J].干旱区研究,1997,14(1):17-27.[7]国务院办公厅.国务院办公厅关于加快木本油料产业发展的意见[EB/OL].(2015-01-13)[2018-01-03].http://www.gov.cn/zhengce/content/2015-01/13/content_9386.htm.[8]姜志娜,谭晓风,袁军,等．油茶果实和叶片中主要营养物质含量变化规律[J].中南林业科技大学学报,2012,32(5):42-45．[9]陈虹.新疆早实核桃果实生长发育影响因素[D].乌鲁木齐:新疆农业大学,2016.[10]MOSCATELLO S,PROIETTI S,AUGUSTI A,et al.Last summer photosynthesis and storage carbohydtates in walnut(Juglans regia L.):Feed-back and feed-forward effects[J].Plant Physiology and Biochemistry,2017,118:618.[11]LACOINTE A,KAJJI A,DAUDET F A,et al.Seasonal variation of photosynthetic carbon flow rate into young walnut and its partitioning among the plant organs and functions[J].Journal of Plant Physiology,1995,146(3):222-230.[12]张上隆,陈昆松.果实品质形成与调控的分子生理[M].北京:中国农业出版社,2007.[13]中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,1999．[14]WANG F,SANZ A,BRENNER M L,et al.Sucrose synthase,starch accumulation and tomato fruit strength[J].Plant Physiology,1993,101(1):321-327.[15]李永涛.核桃胚发育及其营养代谢动态研究[D].泰安:山东农业大学,2011.[16]MORIGUCHI T，SANADA T，YAMAKI S.Seasonal fluctuation of some enzymes relating to sucrose and sorbitol metabolism in peach fruit[J].Journal of the American Society for Horticultural Science,1990,115(2):278-281.[17]BIANCO L,RIEGER M.Partitioning of sorbitol and sucrose catabolism within peach fruit[J].Journal of the American Society for Horticultural Science,2002,127(1):115-121.[18]NONIS A,RUPERTI B,FALCHI R,et al.Differntial expression and regulation of a neutral invertase encoding gene from peach (Prunus persica):Evidence for a role in fruit development[J].Plant Physiology,2007,129(2):436-466.[19]HUBBARD N L,PHARR D M,HUBER S C.Sucrose phosphate synthase and other sucrose metabolizing enzymes in fruits of various species[J].Physilogia Plantarum,1991,82(2):191-196.[20]ZENG Y,WU Y,AVIGNE W T,et al.Rapid repression ot maize invertases by low oxygen.Invertase/sucrose synthase balance,sugar signaling potential,and seedling survival[J].Plant Physiolgy,1999,121(2):599-608.[21]KOCH K.Sucrose metabolism：Regulatory mechanism and pivotal roles in sugar sencing and plant development[J].Current Opinion in Plant Biology,2004,7(3):235-246.[22]BOLOGA K L,FERNIE A R,LEISSE A,et al.A bypass of sucrose synthase leads to low internal oxygen and impaired metabolic performance in growing potato tubers[J].Plant Physiolgy,2003,132(4):436-466.

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备注/Memo

第一作者简介：张翠芳（1984-），女，博士，讲师，硕士生导师，现主要从事果树栽培与生理等研究工作。E-mail：zcf851022@163.com.责任作者：王世伟（1984-），男，博士，副教授，硕士生导师，现主要从事林木栽培与生理等研究工作。E-mail：wsw850204@163.com.基金项目：国家自然科学基金资助项目（31660548）；新疆农业大学国家级大学生创新资助项目(201710758005)。收稿日期：2019-04-28

更新日期/Last Update: 2019-11-26