QIN Qian,LU Zuzheng,DING Liqiong,et al.Identification and Biological Characteristics of the Pathogen Causing Orchid Fusarium Leaf Spot in Guangxi[J].Northern Horticulture,2019,43(18):40-46.[doi:10.11937/bfyy.20190047]
广西兰花镰刀菌叶斑病病原菌鉴定及生物学特性
- Title:
- Identification and Biological Characteristics of the Pathogen Causing Orchid Fusarium Leaf Spot in Guangxi
- 文献标志码:
- A
- 摘要:
- 从广西乐业县雅长兰科植物保护区科普园采集兰花叶斑病的病叶，采用组织分离法分离病原菌，单孢纯化后通过柯赫氏法则验证为致病菌；根据形态学特征，结合多基因系统学鉴定，并测定该菌的碳氮源利用，适宜生长的温度及pH等生物学特性。以此明确兰花叶斑病的病原菌及其生物学特性。结果表明:兰花叶斑病的病原菌鉴定为层出镰刀菌(F.proliferatum)。该菌对温度的适应范围较广，5~37 ℃均能生长，最适生长温度为28 ℃；在一定时间内孢子可以耐受52 ℃的高温。光照对菌丝生长和产孢影响不显著。pH 4~12均能生长和产孢，菌丝生长最适pH为5~9，乳糖、肌醇、纤维素和可溶性淀粉是菌丝生长的最适碳源，蔗糖为产孢的最适碳源；蛋白胨、酵母浸膏、丙氨酸和甘氨酸是生长最适氮源。
- Abstract:
- The spotted leaves were collected from the Yachang Orchidaceae Plant Conservation Popular Science Park,Leye County,Guangxi.The pathogens were isolated by tissue separation method.After being purified by single spore isolation，they were verified as a pathogen by Koch′s law.Then the pathogen was identified by morphological characteristics and polygenic systematic sequencing and analyses.After that,the biological characteristics including the carbon and nitrogen source utilization of the pathogen，suitable temperature and pH for growth were tested.The pathogen was identified as Fusarium proliferatum.This pathogen had adaptability to a wide range of temperature，from 5-37 ℃.During a certain period of time，spores could tolerate the 52 ℃ high temperature.Illumination conditions had no significant effect on the mycelium growth and sporulation of the pathogen.This pathogen could grow and sporulate between pH 4-12.The optimal pH for mycelium growth was 5-9.Lactose，inositol，cellulose and soluble starch were optimal carbon sources for mycelium growth.Sucrose was the optimal carbon source for the spore production.Peptone，yeast extract，alanine and glycine were optimal nitrogen sourced for mycelium growth.
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备注/Memo
第一作者简介：覃茜(1989-)，女，广西南宁人，硕士，助理研究员，现主要从事植物真菌性病害防治等研究工作。E-mail:rzsqqin@163.com.基金项目：广西壮族自治区亚热带作物研究所基本科研业务费专项资助项目（桂热研201704）。收稿日期：2019-03-13