[1]胡艳平,周扬,云天海,等.南瓜HSP70蛋白家族的鉴定和生物信息学分析[J].北方园艺,2019,43(17):7-13.[doi:10.11937/bfyy.20184654]
　HU Yanping,ZHOU Yang,YUN Tianhai,et al.Identification and Bioinformation Analysis of HSP70 Protein Family in Cucurbita moschata[J].Northern Horticulture,2019,43(17):7-13.[doi:10.11937/bfyy.20184654]
点击复制

南瓜HSP70蛋白家族的鉴定和生物信息学分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年17 页码: 7-13 栏目: 出版日期: 2019-09-15
Title:
Identification and Bioinformation Analysis of HSP70 Protein Family in Cucurbita moschata
作者:
胡艳平1; 周扬2; 云天海1; 朱白婢1; 王敏1; 张文1
（1.海南省农业科学院 蔬菜研究所，海南省蔬菜生物学重点实验室，海南省瓜菜育种工程技术研究中心，海南 海口 571100；2.海南大学 园艺学院，海南 海口 570228）
Author(s):
HU Yanping1; ZHOU Yang2; YUN Tianhai1; ZHU Baibi1; WANG Min1; ZHANG Wen1
(1.The Institute of Vegetables,Hainan Academy of Agricultural Sciences,Hainan Key Laboratory of Vegetable Biology,Hainan Vegetable Breeding Engineering Technology Research Center,Haikou,Hainan 571100；2.College of Horticulture,Hainan University,Haikou,Hainan 570228)
关键词:
南瓜; 热激蛋白70; 生物信息学; 启动子分析
Keywords:
Cucurbita moschata; heat shock protein 70; bioinformation; promoter analysis
DOI:
10.11937/bfyy.20184654
文献标志码:
A
摘要:
热激蛋白70(heat shock protein 70,HSP70)是植物应对逆境胁迫产生的一类特定的应激蛋白。以南瓜基因组数据库为基础，利用生物信息学方法对南瓜[STBX]HSP70[STBZ]基因家族进行鉴定分析，并对蛋白特征、基因结构、保守结构域和启动子序列进行研究。结果表明：南瓜基因组数据库中含有10个[STBX]HSP70[STBZ]，编码的蛋白序列长度为572~843个氨基酸，HSP70蛋白含有6~8个保守结构域，可分为4个亚家族。基因结构分析表明[STBX]HSP70[STBZ]具有0~8个内含子。启动子元件分析显示[STBX]HSP70[STBZ]的启动子含有多个信号元件，说明HSP70可能参与多种功能的调控。研究结果为下一步研究HSP70家族蛋白的功能奠定基础。
Abstract:
Heat shock protein 70 (HSP70) is a kind of specific stress protein in response to adversity stress in plant.The [STBX]HSP70[STBZ] gene family was identified based on the pumpkin genome database,and the protein characteristics,gene structures,conserved domains and promoter sequences were analyzed by bioinformation methods.The results showed that there were 10 [STBX]HSP70[STBZ] genes in the database.The sequence lengths of HSP70 proteins were varying from 572 to 843 amino acid residues.The HSP70 proteins contained 6-8 conserved domains and could be clustered into four classes.The intron/exon structural analyses revealed that the [STBX]HSP70[STBZ] genes contain different introns varying from 0 to 8.Several cis-elements were found in the promoter region of [STBX]HSP70[STBZ] genes,indicating that HSP70 might be involved in the regulation of multiple functions.These results lay a foundation for further study on the function of HSP70 family proteinsy in Cucurbita moschata.

参考文献/References:

[1]YAMORI W,HIKOSAKA K,WAY D A.Temperature response of photosynthesis in C3,C4 and CAM plants:Temperature acclimation and temperature adaptation[J].Photosynthesis Research,2014,119:101-117.[2]GUPTA S C,SHARMA A,MISHRA M,et al.Heat shock proteins in toxicology:How close and how far?〖KG-*2〗[J].Life Science,2010,86(11/12):377-384.[3]SWINDELL W R,HUEBNER M,WEBER A P.Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways[J].BMC Genomics,2007,8:125.[4]ALTSCHULER M,MASCCARENHAS J P.Transcription and translation of heat shock and normal proteins in seedlings and developing seeds of soybean exposed to a gradual temperature increase[J].Plant Molecular Biology,1985,5:291-297.[5]杨国正,张秀如,孙湘宁,等.棉花热激蛋白产生规律的初步研究[J].华中农业大学学报,1997(16):18-25.[6]杨金莹,孙颖,孙爱清,等.番茄[STBX]LeHsp110/ClpB[STBZ]基因的分子克隆及其对植物耐热性的影响[J].生物工程学报,2006,22(1):52-57.[7]GROVER A,MITTAL D,NEGI M,et al.Generating high temperature tolerant transgenic plants:Achievements and challenges[J].Plant Science,2013,205:38-47.[8]QUEITSCH C,SANGSTER T A,LINDQUIST S.Hsp90 as a capacitor of phenotypic variation[J].Nature,2002,417:618-624.[9]XU J,XUE C,XUE D,et al.Overexpression of GmHsp90s,a heat shock protein 90 (Hsp90) gene family cloning from soybean,decrease damage of abiotic stresses in Arabidopsis thaliana[J].PLoS One,2013,8(7):e69810.[10]TOMINAGA H,COURY D A,AMANO H,et al.cDNA cloning and expression analysis of two heat shock protein genes,[STBX]Hsp90 and Hsp60[STBZ],from a sterile Ulva pertusa (Ulvales,Chlorophyta)[J].Fisheries Science,2012,78(2):415-429.[11]MAIMBO M,OHNISHI K,HIKICHI Y,et al.Induction of a small heat shock protein and its functional roles in Nicotiana plants in the defense response against Ralstonia solanacearum[J].Plant Physiology,2014,145(4):1588-1599.[12]SU P H,LI H.Arabidopsis stromal 70-kD heat shock proteins are essential for plant development and important for thermotolerance of germinating seeds[J].Plant Physiology,2008,146(3):1231-1241.[13]KIM S R,AN G.Rice chloroplast-localized heat shock protein 70,OsHsp70CP1,is essential for chloroplast development under high temperature conditions[J].Journal of Plant Physiology,2013,170(9):854-863.[14]SONG A,ZHU X,CHEN F,et al.A Chrysanthemum heat shock protein confers tolerance to abiotic stress[J].International Journal of Molecular Science,2014,15:5063-5078.[15]SUN H,WU S,ZHANG G,et al.Karyotype stability and unbiased fractionation in the paleo-allotetraploid Cucurbita genomes[J].Molecular Plant,2017,10:1293-1306.[16]RITOSSA E.A new puffing pattern induced by temperature shock and DNP in Drosophila[J].Experientia,1962,18:571-573.[17]LARGE A T,GOLDBERG M D,LUND P A.Chaperones and protein folding in the archaea[J].Biochemical Society Transaction,2009,37:46-51.[18]SUNG D,VIERLING E,GUY C L.Comprehensive expression profile analysis of the Arabidopsis [STBX]HSP70[STBZ] gene family[J].Plant Physiology,2001,126(2):789-800.[19]陈旭,石垒,朱璐,等.植物HSP70蛋白家族分子进化特征及其表达模式分析[J].基因组学与应用生物学,2017,36(10):4282-4294.[20]王荣青,万红建,李志邈,等.番茄[STBX]HSP70[STBZ]基因鉴定及系统发育关系分析[J].核农学报,2014,28(3):378-385.

相似文献/References:

[1]李会彬,赵玉靖,王丽宏,等.冀西北高原食用南瓜平衡施肥研究[J].北方园艺,2013,37(13):197.
　LI Hui-bin,ZHAO Yu-jing,WANG Li-hong,et al.Study on Balanced Fertilization of Pumpkins in Northwest Plateau of Hebei Province[J].Northern Horticulture,2013,37(17):197.
[2]杨欢欢,李景富.不同砧木黄瓜/南瓜嫁接愈合过程的解剖学观察[J].北方园艺,2014,38(08):5.
　YANG Huan-huan,LI Jing-fu.The Anatomical Observation of Different Root Stock of Healing Process on the Grafted Cucumber/Pumpkin[J].Northern Horticulture,2014,38(17):5.
[3]孟令君,李柱刚,韩俊岩,等.南瓜SSR-PCR反应体系的优化[J].北方园艺,2013,37(10):103.
　MENG Ling-jun,LI Zhu-gang,HAN Jun-yan,et al.Optimization for SSR-PCR Technology System in Cucurbita moschata[J].Northern Horticulture,2013,37(17):103.
[4]王玉敏.南 瓜 卷 的 研 制[J].北方园艺,2012,36(01):148.
　WANG Yu-min.The Development of Pumpkin Sweetend Roll[J].Northern Horticulture,2012,36(17):148.
[5]刘振威,李新峥.南瓜优势杂交组合（F1）的评价研究[J].北方园艺,2013,37(08):17.
　LIU Zhen-wei,LI Xin-zheng.Study on the Evaluation of Heterotic Combinations of Pumpkin[J].Northern Horticulture,2013,37(17):17.
[6]王玉敏.香脆南瓜条的研制[J].北方园艺,2012,36(03):154.
　WANG Yu-min.Research on Crispy Pumpkin Piece[J].Northern Horticulture,2012,36(17):154.
[7]傅永斌,任全军,奚玉银,等.不同沟垄种植形式对旱坡地南瓜生长及产量的影响[J].北方园艺,2012,36(17):5.
　FU Yong-bin,REN Quan-jun,XI Yu-yin,et al.Effects of Different Ridge Planting Patterns on Growth and Yield of Pumpkin in Sloping Dryland[J].Northern Horticulture,2012,36(17):5.
[8]肖仲久,李小霞,李斌.贵州省南瓜白粉病病原菌初步鉴定[J].北方园艺,2012,36(09):146.
　XIAO Zhong-jiu,LI Xiao-xia,LI Bin.Preliminary Identification of Powdery Mildew on Pumpkin in Guizhou[J].Northern Horticulture,2012,36(17):146.
[9]杜黎君,孙丽,李新峥,等.南瓜田间气象因子时间变化规律及对光合速率的影响[J].北方园艺,2014,38(15):25.
　DU Li-jun,SUN Li,LI Xin-zheng-,et al.Daily Variation Rule of Weather Factors and Its Affection on Photosynthetic Rate in Pumpkin Field[J].Northern Horticulture,2014,38(17):25.
[10]温玲,赵丹,朱子成,等.俄罗斯南瓜种质资源引进及综合性状评价[J].北方园艺,2015,39(19):11.[doi:10.11937/bfyy.201519003]
　WEN Ling,ZHAO Dan,ZHU Zicheng,et al.Introduction and Comprehensive Characters Evaluation of Russia’s Pumpkin Germplasm[J].Northern Horticulture,2015,39(17):11.[doi:10.11937/bfyy.201519003]

备注/Memo

第一作者简介：胡艳平(1988-)，女，硕士，助理研究员，研究方向为蔬菜分子生物学。E-mail:ziy2013@163.com.责任作者：张文(1968-)，男，硕士，研究员，研究方向为作物营养与栽培学。E-mail：hnzhangwen@163.com.基金项目：海南省瓜菜育种工程技术研究中心开放课题资助项目(GCYZGCZX2017001)；海南省重点实验室和工程技术研究中心建设专项资助项目(JSZX009)。收稿日期：2019-03-15

更新日期/Last Update: 2019-09-20