[1]胡艳平,周扬,云天海,等.南瓜HSP70蛋白家族的鉴定和生物信息学分析[J].北方园艺,2019,43(17):7-13.[doi:10.11937/bfyy.20184654]
　HU Yanping,ZHOU Yang,YUN Tianhai,et al.Identification and Bioinformation Analysis of HSP70 Protein Family in Cucurbita moschata[J].Northern Horticulture,2019,43(17):7-13.[doi:10.11937/bfyy.20184654]
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南瓜HSP70蛋白家族的鉴定和生物信息学分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年17 页码: 7-13 栏目: 出版日期: 2019-09-15
Title:
Identification and Bioinformation Analysis of HSP70 Protein Family in Cucurbita moschata
作者:
胡艳平1; 周扬2; 云天海1; 朱白婢1; 王敏1; 张文1
（1.海南省农业科学院 蔬菜研究所，海南省蔬菜生物学重点实验室，海南省瓜菜育种工程技术研究中心，海南 海口 571100；2.海南大学 园艺学院，海南 海口 570228）
Author(s):
HU Yanping1; ZHOU Yang2; YUN Tianhai1; ZHU Baibi1; WANG Min1; ZHANG Wen1
(1.The Institute of Vegetables,Hainan Academy of Agricultural Sciences,Hainan Key Laboratory of Vegetable Biology,Hainan Vegetable Breeding Engineering Technology Research Center,Haikou,Hainan 571100；2.College of Horticulture,Hainan University,Haikou,Hainan 570228)
关键词:
南瓜; 热激蛋白70; 生物信息学; 启动子分析
Keywords:
Cucurbita moschata; heat shock protein 70; bioinformation; promoter analysis
DOI:
10.11937/bfyy.20184654
文献标志码:
A
摘要:
热激蛋白70(heat shock protein 70,HSP70)是植物应对逆境胁迫产生的一类特定的应激蛋白。以南瓜基因组数据库为基础，利用生物信息学方法对南瓜[STBX]HSP70[STBZ]基因家族进行鉴定分析，并对蛋白特征、基因结构、保守结构域和启动子序列进行研究。结果表明：南瓜基因组数据库中含有10个[STBX]HSP70[STBZ]，编码的蛋白序列长度为572~843个氨基酸，HSP70蛋白含有6~8个保守结构域，可分为4个亚家族。基因结构分析表明[STBX]HSP70[STBZ]具有0~8个内含子。启动子元件分析显示[STBX]HSP70[STBZ]的启动子含有多个信号元件，说明HSP70可能参与多种功能的调控。研究结果为下一步研究HSP70家族蛋白的功能奠定基础。
Abstract:
Heat shock protein 70 (HSP70) is a kind of specific stress protein in response to adversity stress in plant.The [STBX]HSP70[STBZ] gene family was identified based on the pumpkin genome database,and the protein characteristics,gene structures,conserved domains and promoter sequences were analyzed by bioinformation methods.The results showed that there were 10 [STBX]HSP70[STBZ] genes in the database.The sequence lengths of HSP70 proteins were varying from 572 to 843 amino acid residues.The HSP70 proteins contained 6-8 conserved domains and could be clustered into four classes.The intron/exon structural analyses revealed that the [STBX]HSP70[STBZ] genes contain different introns varying from 0 to 8.Several cis-elements were found in the promoter region of [STBX]HSP70[STBZ] genes,indicating that HSP70 might be involved in the regulation of multiple functions.These results lay a foundation for further study on the function of HSP70 family proteinsy in Cucurbita moschata.

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备注/Memo

第一作者简介：胡艳平(1988-)，女，硕士，助理研究员，研究方向为蔬菜分子生物学。E-mail:ziy2013@163.com.责任作者：张文(1968-)，男，硕士，研究员，研究方向为作物营养与栽培学。E-mail：hnzhangwen@163.com.基金项目：海南省瓜菜育种工程技术研究中心开放课题资助项目(GCYZGCZX2017001)；海南省重点实验室和工程技术研究中心建设专项资助项目(JSZX009)。收稿日期：2019-03-15

更新日期/Last Update: 2019-09-20