LI Yalan,PAN Cunde,CHEN Hong.(Collage of Forestry and Horticulture，Xinjiang Agricultural University/Key Laboratory of Forestry Ecology and Industry Technology in Arid Region，Education Department of Xinjiang，Urumqi，Xinjiang 830052)[J].Northern Horticulture,2019,43(16):44-52.[doi:10.11937/bfyy.20184462]
SRAP标记在五十份新疆人工栽培核桃种质资源的鉴定与应用
- Title:
- (Collage of Forestry and Horticulture，Xinjiang Agricultural University/Key Laboratory of Forestry Ecology and Industry Technology in Arid Region，Education Department of Xinjiang，Urumqi，Xinjiang 830052)
- Keywords:
- walnut; SRAP-PCR; heat map; multiple regression trees; R language
- 文献标志码:
- A
- 摘要:
- 以新疆核桃主栽区收集的50份核桃种质资源为试材，采用序列相关扩增多态性（sequence-related amplified polymorphism，SRAP）标记技术分析其遗传多样性，以期为分布于新疆地区的核桃种质资源的保存、鉴定及综合开发利用提供参考依据。结果表明：从100对引物中筛选出16对扩增条带清晰、稳定性好的多态性SRAP引物组合，16对引物组合在50份供试种质中共扩增出178条带，多态性条带135条，多态位点百分率为75.84%。聚类分析及聚类锐度测试显示供试种质可分为10类，热图显示出不同引物对种质的区分情况，多元回归树显示不同引物中的26个扩增条带能够区分50份供试种质，其中M4/E4、M7/E5、M14/E4、M2/E11、M3/E4、M2/E4、M12/E13、M7/E1、M14/E13和M1/E3共10对引物组合是供试种质鉴定中重要的引物组合。同时R语言在统计分析中的应用，为今后核桃植物形态与遗传变异研究提供了新的数据分析方法。
- Abstract:
- The 50 walnut germplasms from Xinjiang were used as experimental materials in this study.The genetic diversity of 50 walnut germplasms were analysed by SRAP(Sequence-related amplified polymorphism) markers in order to provide reference for the preservation，identification and comprehensive development of walnut germplasm resources distributed in Xinjiang.The results showed that 16 SRAP primer combinations amplified 178 bands，135 polymorphic bands，75.84% polymorphism ratio.The cluster analysis and cluster sharpness tests showed that the test germplasm was divided into 5 groups，and the heat map displayed different primers were used to distinguish germplasm.Multiple regression trees showed that 12 amplified bands in different primers could distinguish 50 accessions.The above studies indicate that the genetic diversity of the tested walnut germplasm was abundant，and the selected SRAP primer combinations are effective for distinguishing the tested germplasm，among which M4/E4,M7/E5,M14/E4,M2/E11,M3/E4,M2/E4,M12/E13,M7/E1,M14/E13 and M1/E3 ten primer combinations were important primer combinations for the identification of germplasm for testing，which can provide basic data for basic theoretical research and breeding research of walnut germplasm resources.At the same time，the application of R language in the statistical analysis of this paper provides a new data analysis method for the future R language applied to the study of walnut plant morphology and genetic variation.
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备注/Memo
第一作者简介：李亚兰（1989-），女，新疆博湖人，博士研究生，研究方向为果树种质资源。E-mail：weixiaoliyalan@163.com.责任作者：潘存德（1964-），男，新疆奇台人，博士，教授，博士生导师，研究方向为森林生态与经营。E-mail：pancunde@163.com.基金项目：国家自然科学基金资助项目（31760219，31460210）。收稿日期：2019-03-20