CHEN Liang,CHEN Nianlai,SUN Xiaomei,et al.Identification of Resistance to Fusarium Wilt of Watermelon Grafted Rootstocks and Hybrid[J].Northern Horticulture,2019,43(15):15-22.[doi:10.11937/bfyy.20184314]
西瓜嫁接砧木资源及其杂交组合枯萎病抗性鉴定
- Title:
- Identification of Resistance to Fusarium Wilt of Watermelon Grafted Rootstocks and Hybrid
- Keywords:
- watermelon; Fusarium wilt; physiological race; rootstock materials; artificial inoculation
- 文献标志码:
- A
- 摘要:
- 为了筛选西瓜枯萎病抗性砧木材料，对近年来收集整理的部分西瓜亲本材料及选配的砧木组合进行苗期枯萎病抗性人工接种鉴定。以国际上公认的5个西瓜枯萎病鉴别寄主材料（‘Black Diamond’‘Crimson Sweet’‘Charleston Gray’‘Calhoun Gray’‘PI296341-FR’）以及选配的49份西瓜砧木资源为试材，采用尖镰孢菌（Fusarium oxysporum f.sp.niveum）孢子悬浮液人工接种法，鉴定试验所用的西瓜枯萎病菌生理小种和砧木材料的枯萎病抗性。结果表明：鉴别寄主‘Black Diamond’‘Crimson Sweet’‘Charleston Gray’‘Calhoun Gray’ ‘PI296341-FR’的病情指数分别达85.2、53.0、27.2、19.2和7.8，抗性级别分别为感病、轻抗、中抗和抗病；49份西瓜砧木材料中，筛选出对Race 1表现抗病的材料有10份，占总供试材料的20.4%，中抗材料29份，占59.2%，轻抗材料10份，占20.4%，不存在免疫和完全感病的材料。确认该试验所用尖孢镰刀菌为生理小种1，筛选确定了高抗西瓜枯萎病的4份亲本材料588、893、BEH和FR328，6份杂交材料威砧101、Be-3×893、(BEH-15×Sugarlee)×893、(BEH1-15×Sugarlee)×341、(BEH1-15×AW2-1)×341和(BYH-1×Sugarlee)×893，可为设施西瓜生产提供优良的嫁接砧木资源。
- Abstract:
- To screen the resistance to wilt disease of rootstock resources of watermelon,the resistance to wilt disease of some the watermelon parent materials and their hybrid rootstock combinations were identified by artificial inoculation method in seedling stage.The internationally recognized five watermelon wilt identification host materials(‘Black Diamond’‘Crimson Sweet’‘Charleston Gray’‘Calhoun Gray’‘PI296341-FR’) and the selected 49 rootstock resources of watermelon were identified by Fusarium oxysporum f.sp.niveum spore suspension methods.The results showed that the DI of‘Black Diamond’‘Crimson Sweet’‘Charleston Gray’‘Calhoun Gray’‘PI296341-FR’ was 85.2,53.0,27.2,19.2 and 7.8 respectively,the resistance levels were susceptibility,light resistance,moderate resistance and resistance.10 watermelon rootstock materials(about 20.4% resources) showed resistance to Race 1,29 materials(about 59.2% resources)showed moderate resistance,10 materials(about 20.4% resources) showed light resistance,the immunization and high susceptible resources was not obtained.The results showed that the watermelon wilt pathogen was Race 1 in this experiment,4 parental materials 588,893,BEH and FR328,6 hybrid materials Wei-zhen101,Be-3×893,(BEH-15×Sugarlee)×893,(BEH1-15×Sugarlee)×341,(BEH1-15×AW2-1)×341 and (BYH-1×Sugarlee)×893 showed high resistance to watermelon wilt,and they could be used as excellent grafting rootstock resources for facility watermelon production in future.
参考文献/References:
[1]谭素英,贾文海.无籽西瓜栽培与育种[M].北京:中国农业出版社,2000,40-90.[2]马跃.2007年全国西瓜生产回顾与2008年产销预测[J].长江蔬菜,2008,23(1):54-56.[3]张友青.设施西瓜主要病害特征及防治技术[J].河北农业,2017(7):48-50.[4]ZHANG Z,ZHANG J,WANG Y,et al.Molecular detection of Fusarium oxysporum f.sp.niveum and Mycosphaerella melonis infected plant tissues and soil[J].FEMS Microbiol Lett,2005,249(1):39-47.[5]张屹,魏林,许勇,等.西瓜枯萎病的研究进展[J].湖南农业科学,2013(5):67-70.[6]赵宣.西瓜枯萎病的研究进展[J].农村经济与科技,2018,29(2):26-29.[7]肖光辉.西瓜抗枯萎病育种研究进展[J].湖南农业科学,2001(5):43-45.[8]羊杏平.西瓜枯萎病抗性基因遗传分析、分子标记及育种研究[D].南京:南京农业大学,2012.[9]耿丽华,郭绍贵,吕桂云,等.西瓜枯萎病菌生理小种鉴定技术体系的建立和验证[J].中国蔬菜,2010(20):52-56.[10]林柏青,张松林.辣椒品种抗疫病鉴定方法初步研究[J].中国蔬菜,1994(4):21-24.[11]潘存祥.西瓜抗枯萎病生理生化机理的研究[D].兰州:甘肃农业大学,2014.[12]MARTYN R.An aggressive race of Fusarium oxysporum f.sp. niveum new to the United States[J].Plant Disease,1985,69:1007.[13]MARTYN R D,MCLAUGHLIN R J.Effects of inoculum concentration on the apparent resistance of watermelons to Fusarium oxysporum f.sp.niveum[J].Plant Disease,1983,67：493-495.[14]ZHANG X W,HUANG X S,GU Q S.A preliminary report on screening the resistance of watermelon varieties to Fusarium wilt[J].Acta Hort,1995,402:45-47.[15]段会军,马峙英,张彩英,等.河北省西瓜枯萎病菌生理小种分化及其分布[D].保定:河北农业大学,2005.[16]顾卫红,王燕华,宋荣浩.上海地区西瓜枯萎病病原菌生理小种初探[J].上海农业学报,1994(3):63-67.[17]王冬梅,王纯利,曲丽红,等.新疆西瓜枯萎病菌生理小种初探[J].新疆农业大学学报,1998,21(2):115-118.[18]张兴平,王鸣.我国西瓜枯萎病生理小种分化研究初报[J].中国西瓜甜瓜,1991(1):39-43.[19]NETZER.Inheritance of resistance in watermelon to races of Fusarium oxysporurn[J].Plant Disease,1980,164(9):853-854.[20]CRALL.Historyand present status of water melon improvement by breeding[J].Soil Sci,1953,13:71-74.[21]HENDERSON.The inheritance of Fusarium wilt resistance in watermelon[J].Jam Soc Hort Sci,1970,95:276-282.[22]黄学森,张学炜,焦定量,等.西瓜部分品种抗枯萎病特性在F1代中遗传表现的初步探讨[J].中国西瓜甜瓜,1991(2):11-15.[23]于利,徐润芳,赵有为.西瓜品种抗枯萎病遗传研究[J].江苏农业学报,1995,11(1):45-48.[24]周凤珍,康国斌.西瓜抗枯萎病品种“卡红”的抗病遗传研究[J].植物病理学报,1996,26(3):261-262.
相似文献/References:
[1]林燚,王驰,杨瑜斌,等.西瓜嫁接栽培植株凋萎原因及对策[J].北方园艺,2013,37(04):59.
[2]陈 潇,盛萍萍,刘润进,等.西瓜与辣椒间作体系接种AM真菌对连作西瓜植株[J].北方园艺,2014,38(12):6.
CHEN Xiao,SHENG Ping-ping,LIU Run-jin,et al.Effect of Intercropping System of Pepper-Watermelon Crops Inoculating Arbuscular Mycorrhizal Fungus on Growth and Defence Enzymes Activity of the Continuous Cropping Watermelon[J].Northern Horticulture,2014,38(15):6.
[3]李 勤,皇甫自起,陈大伟.豫东地区地膜覆盖栽培西瓜品种比较试验[J].北方园艺,2014,38(12):23.
LI Qin,HUANGFU Zi-qi,CHEN Da-wei.Comparison Test on Film Mulching Cultivating Watermelon Varieties in the East of Henan Province[J].Northern Horticulture,2014,38(15):23.
[4]王林闯,汪翠芳,罗德旭,等.木薯渣复合基质对西瓜幼苗生长的影响[J].北方园艺,2014,38(04):5.
WANG Lin-chuang,WANG Cui-fang,LUO De-xu,et al.Effect of Compound Substrate With Cassava Dregs on the Growth of Watermelon Seedling[J].Northern Horticulture,2014,38(15):5.
[5]郭松,刘声峰,于 蓉,等.压砂地西瓜水肥耦合模型及优化组合方案[J].北方园艺,2013,37(17):8.
GUO Song,LIU Sheng-feng,YU Rong,et al.Optimized Combination Scheme and Water-Fertilizer Coupling Model on Watermelon in Sand Field[J].Northern Horticulture,2013,37(15):8.
[6]徐雪莲,张银东,符悦冠,等.蚜虫侵害对西瓜保护酶活性的影响[J].北方园艺,2014,38(01):109.
XU Xue-lian,ZHANG Yin-dong,FU Yue-guan,et al.Effect of Aphid Invasion on Protective Enzyme Activities of Different Watermelon Varieties[J].Northern Horticulture,2014,38(15):109.
[7]韩亚楠,毕美光,刘润进,等.AM真菌对连作西瓜生长及其枯萎病的影响[J].北方园艺,2013,37(13):150.
HAN Ya-nan,BI Mei-guang,LIU Run-jin,et al.Effects of Arbuscular Mycorrhizal Fungus on Growth and Fusarium Wilt of Watermelon Seedlings in Continuous Cropping Soils[J].Northern Horticulture,2013,37(15):150.
[8]杜社妮,白岗栓,边利军.陕北丘陵沟壑区西瓜套种向日葵栽培技术[J].北方园艺,2014,38(02):56.
DU She- ni,BAI Gang- shuan,BIAN Li- jun.Cultivation Technology of Watermelon/Sunflower Intercropping in Loess Hilly and Gully Region of Northern Shaanxi[J].Northern Horticulture,2014,38(15):56.
[9]冯 雪,梁奎景,王 茜,等.合欢枯萎病病原菌的生物学特性研究[J].北方园艺,2014,38(02):128.
FENG Xue,LIANG Kui-jing,WANG Qian,et al.Study on the Biological Characteristics of Pathogen of Fusarium Wilt of Albizzia julibrissin Durazz[J].Northern Horticulture,2014,38(15):128.
[10]田 梅,于 蓉,董 瑞,等.压砂地不同西瓜品种光合作用日变化研究[J].北方园艺,2013,37(23):27.
TIAN Mei,YU Rong,DONG Rui,et al.Study on Photosynthetic Diurnal Variation of Different Cultivars of Watermelon in Gravel-mulched Field[J].Northern Horticulture,2013,37(15):27.
[11]吕湘江,李清萍,范淑英.西瓜枯萎病综合防治研究进展[J].北方园艺,2015,39(06):187.[doi:10.11937/bfyy.201506051]
LYU Xiang-jiang,LI Qing-ping,FAN Shu-ying.Research Advance in Integrated Management of Watermelon Fusarium Wilt[J].Northern Horticulture,2015,39(15):187.[doi:10.11937/bfyy.201506051]
[12]黄业昌,孙吉庆,陈勇兵.邻苯二甲酸二丁酯对西瓜光合作用及枯萎病的影响[J].北方园艺,2017,41(04):111.[doi:10.11937/bfyy.201704026]
HUANG Yechang,SUN Jiqing,CHEN Yongbing.Effects of Dibutyl Phthalate on Photosynthesis and Fusarium Wilt of Watermelon[J].Northern Horticulture,2017,41(15):111.[doi:10.11937/bfyy.201704026]
[13]刘柳,南宇航,沙彤芸,等.西瓜种质资源对枯萎病和白粉病的抗性评价[J].北方园艺,2018,42(11):43.[doi:10.11937/bfyy.20174762]
LIU Liu,NAN Yuhang,SHA Tongyun,et al.Evaluation of Watermelon Germplasm Resistant to Fusarium Wilt and Powdery Mildew[J].Northern Horticulture,2018,42(15):43.[doi:10.11937/bfyy.20174762]
[14]王志强,刘声锋,尚建立,等.西瓜种质资源枯萎病抗性鉴定[J].北方园艺,2019,43(13):73.[doi:10.11937/bfyy.20184205]
WANG Zhiqiang,LIU Shengfeng,SHANG Jianli,et al.Identification of Resistance to Fusarium Wilt in Watermelon Germplasm[J].Northern Horticulture,2019,43(15):73.[doi:10.11937/bfyy.20184205]
备注/Memo
第一作者简介：陈亮(1989-)，男，甘肃武威人，硕士，研究实习员，现主要从事设施蔬菜生理与栽培技术等研究工作。E-mail:chenliang144358@163.com.责任作者：李金霞（1985-），女，甘肃天祝人，硕士，讲师，现主要从事作物生理生态学等研究工作。E-mail：lijinxia10000@163.com.基金项目：甘肃省科技厅青年资助项目（18JR3RA187）。收稿日期：2019-03-19