XIAN Hongkang,ZHI Qiujuan,LI Hui,et al.Direct Regeneration of Adventitious Buds From Leaves of Rosa laevigata Michx.[J].Northern Horticulture,2019,43(13):93-100.[doi:10.11937/bfyy.20183979]
金樱子（Rosa laevigata Michx.）叶片直接再生不定芽体系的建立
- Title:
- Direct Regeneration of Adventitious Buds From Leaves of Rosa laevigata Michx.
- 文献标志码:
- A
- 摘要:
- 以金樱子组培苗的划伤叶片为外植体，研究了不同植物生长调节剂配比组合、暗培养时间、添加物L-脯氨酸、叶片不同部位对不定芽直接再生的影响。结果表明：当TDZ浓度为1.5 mg?L-1、NAA浓度为0.000 5 mg?L-1时，不定芽直接发生率最高，为9.5%；不同暗培养时间对不定芽直接诱导具有一定影响，暗培养时间为10 d时不定芽的直接发生率最高，为10.5%；添加了不同浓度的L-脯氨酸后不定芽的诱导率不增反降，所以以不添加L-脯氨酸为宜；叶片不同部位的再生率比较中，仅带叶叶柄可直接诱导出不定芽。综上所述，叶片直接再生不定芽的诱导方法是，以金樱子带叶叶柄为外植体，在直接诱导培养基上1/2MS+TDA 1.5 mg?L-1+NAA 0.000 5 mg?L-1+CH 100 mg?L-1+AgNO3 10 mg?L-1+蔗糖30 g?L-1+琼脂7.5 g?L-1，暗培养10 d后，转至正常光周期下培养3周左右，不定芽诱导率最高，为10.5%。所得不定芽在增殖培养基MS+NAA 0.1 mg?L-1+6-BA 1.0 mg?L-1生长3周后，增殖系数可达到3.5左右；将丛生芽切割成单芽后转入不含任何激素的MS培养基壮苗3周，幼苗可长高至3~5 cm；再转入MS+NAA 0.1 mg?L-1培养基中生根，1个月后生根率达95%。
- Abstract:
- The scratched leaves of tissue culture seedlings of Rosa laevigata Michx.were used as explants,and the effects of different combinations of plant growth regulators，dark culture time，additives L-proline and different parts of leaves on the direct regeneration of adventitious buds were studied.The results showed that TDZ concentration was 1.5 mg?L-1 and NAA concentration was 0.000 5 mg?L-1，the direct incidence of adventitious buds was the highest，which was 9.5%.Different dark culture time had certain influence on the direct induction of adventitious buds.The direct incidence of adventitious buds was the highest when dark culture time was 10 days，which was 10.5%.The induction rate of adventitious buds did not increase or decrease after adding different concentrations of L-proline.In comparison of regeneration rate of different parts of leaves，adventitious buds could be directly induced only with petioles.In conclusion，the method of inducing adventitious buds from leaves to regenerate directly was as follows：1/2MS+TDA 1.5 mg?L-1+NAA 0.000 5 mg?L-1+CH 100 mg?L-1+AgNO3 10 mg?L-1+sucrose 30 g?L-1+agar 7.5 g?L-1 on the direct induction medium with petioles.After 10 days of dark culture，the induction rate of adventitious buds was the highest (10.5%).The adventitious buds in the proliferation medium MS+NAA 0.1 mg?L-1+6-BA 1.0 mg?L-1grew after 3 weeks，the proliferation coefficient could reach about 3.5.Then the cluster buds were cut into single buds and transferred to MS medium to sound seedlings for 3 weeks.Subsequently，the resulted 3-5 cm seedlings were then transfer to MS+NAA 0.1 mg?L-1 medium for rooting,andthe post-rooting ratereached 95% one month later.
参考文献/References:
[1]刘学贵,李佳骆,高品一,等.药食两用金樱子的研究进展[J].食品科学,2013,34(11):392-398.[2]李时珍.本草纲目:校点本.下册[M].北京:人民卫生出版社,2005.[3]邹洪涛,杨艳,陈世军.金樱子的饮料开发资源价值与展望[J].食品工业科技,2006(10):193-195.[4]高莉萍.月季品种‘萨蔓莎’植株再生体系的建立和根癌农杆菌介导的遗传转化研究[D].武汉:华中农业大学,2005.[5]任桂芳,王建红,冯慧,等.现代月季(Rosa hybrida)叶片植株再生体系的建立[J].园艺学报,2004,31(4):533-536.[6]赵培培,车代弟,王金刚,等.丰花月季再生体系的建立[J].东北农业大学学报,2008,39(6):30-32.[7]孟令宁,晏慧君,张颢,等.大花香水月季再生体系的初步建立[J].云南农业大学学报（自然科学版）,2012,27(6):870-874.[8]郭艳超,田传卫,尚爱芹,等.粉团蔷薇叶片不定芽的直接诱导及植株再生[J].分子植物育种,2006(s2):27-30.[9]DUBOIS L A M,DE VRIES D P.The direct regeneration of adventitious of rose cultivars for direct shoot organogenesis[J].Acta Horticulturae,1997,447:79-83.[10]VARUTHARAJ K,SOUNDAR R C,THILIP C,et al.High efficiency direct shoot organogenesis from leaf segments of Aerva lanata (L.) Juss.ex Schult by using thidiazuron[J].The Scientific World Journal,,2014(2):1-6.[11]武维华.植物生理学[M].2版.北京:科学出版社,2008.[12]林毅雁.‘月月粉’的再生及刺玫月季育种研究[D].北京:北京林业大学,2010.[13]张建甫,毕玲,陈晓丽,等.蔷薇属植物类根体和类原球茎发生发育的影响因子[J].中国农业科学,2011,44(22):4645-4652.[14]王蒂.植物组织培养[M].北京:中国农业出版社,2004.[15]吴雅琴,赵艳华,李春敏.昌红苹果离体叶片不定芽的诱导及植株再生[J].云南农业大学学报(自然科学版),2006,21(1):32-35.[16]刘翠琼,汤浩茹,罗娅.不同基因型梨叶片离体培养和植株再生[J].园艺学报,2005,32(6):1080-1083.[17]鞠志国.莱阳茌梨细胞中多酚氧化酶的分布及其性质(简报)[J].植物生理学报,1989(3):33-35.[18]李纯佳,张颢,周宁宁,等.大花香水月季茎段组织培养的抗褐化研究[J].西南农业学报,2012,25(3):1047-1050.[19]杨博,韩振海,张永,等.不同光照强度对玫瑰组织培养中初代培养物褐化的影响[J].中国农学通报,2003,19(6):194-196.
相似文献/References:
[1]曹世睿,张婷,王其刚,等.八个蔷薇属种质资源的核型分析[J].北方园艺,2021,(13):85.[doi:10.11937/bfyy.20204689]
CAO Shirui,ZHANG Ting,WANG Qigang,et al.Karyological Analysis on Eight Gemplasm Resources in Genus Rosa L.[J].Northern Horticulture,2021,(13):85.[doi:10.11937/bfyy.20204689]
备注/Memo
第一作者简介：咸宏康（1995-），女，硕士研究生，研究方向为蔷薇科植物生理学。E-mail：hkxian1995@163.com.责任作者：王长泉（1970-），男，博士，教授，博士生导师，现主要从事观赏植物的花期调控和花器官发育等研究工作。E-mail：cqwang@njau.edu.cn.基金项目：江苏省重点研发资助项目（BE2016377）；常州市科技支撑计划资助项目（20170396）。收稿日期：2019-02-18