HUANG Yuntong,LOU Hu,LIU Xue,et al.Extraction Process and Antioxidant Activity of Total Flavonoids From the Bulbs of Multiplier Onion (Allium cepa L.var.Aggregatum G.Don)[J].Northern Horticulture,2019,43(14):106-116.[doi:10.11937/bfyy.20183359]
分蘖洋葱鳞茎总黄酮提取工艺及抗氧化活性
- Title:
- Extraction Process and Antioxidant Activity of Total Flavonoids From the Bulbs of Multiplier Onion (Allium cepa L.var.Aggregatum G.Don)
- Keywords:
- multiplier onion; total flavonoids; ultrasonic extraction; response surface methodology; antioxidant activity
- 文献标志码:
- A
- 摘要:
- 以分蘖洋葱‘M-3’鳞茎为试材，用超声波辅助提取黄酮类化合物，在单因素试验基础上，采用Box-Behnken响应面法优化分蘖洋葱总黄酮提取工艺，用DPPH法对总黄酮化合物抗氧化活性进行评价。结果表明：最佳提取工艺参数为料液比1∶45 g?mL-1、乙醇体积分数60%、提取时间40 min、提取温度48 ℃、超声波功率310 W。在该条件下，分蘖洋葱总黄酮的提取率为3.584%，与模型预测值3.622%相近。分蘖洋葱总黄酮提取物对DPPH?具有一定的清除作用，IC50值为0.179 mg?mL-1。优选的超声波提取工艺提高了分蘖洋葱总黄酮提取率，具有较强的抗氧化活性。
- Abstract:
- Taking the bulbs of ‘M-3’ multiplier onion (Allium cepa L.var.Aggregatum G.Don) as test material,on the basis of single factor experiment,the optimum ultrasound-assisted extraction process of total flavonoids from bulbs was analyzed by using Box-Behnken design (BBD) with response surface methodology.The antioxidant activity of total flavonoids was evaluated by DPPH method.The results showed that the optimal extraction conditions were determined as follows：1∶40 g?mL-1 of solid-liquid ratio,60% of ethanol volume fraction，50 ℃ of extraction temperature，300 W of ultrasonic power，30 minutes of extraction time.Under these conditions，the exaction rate of total flavonoids from the bulbs of multiplier onion was 3.586%，which was closed to the model predicted value of 3.622%.The antioxidant tests showed the total flavonoids had a scavenging effect on DPPH?.The IC50 value was 0.179 mg?mL-1 which indicated that the flavonoids from multiplier onion bulbs has strong antioxidant activity.The optimal ultrasonic-assisted extraction process improved the extraction rate of total flavonoids from the bulbs of multiplier onion，and the extracts by this process also exhibited a better antioxidant activity.
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备注/Memo
第一作者简介：黄云彤（1972-），女，硕士，副教授，研究方向为病原微生物防控。E-mail：qijiangxu@126.com.责任作者：徐启江（1969-），男，博士，教授，研究方向为植物分子生物学。E-mail：qijiangxu@nefu.edu.cn.基金项目：黑龙江省科学基金资助项目（C2018002）；哈尔滨市应用技术研究与开发项目-中小企业创新基金资助项目（2016FF6GJ009）。收稿日期：2019-03-04