DU Zexuan,WU Jingchen,YANG Ruhua,et al.Single and Combined Effects of Vanadium,Titanium and Cadmium on the Physiology and Biochemistry of Marchantia polymorpha L.[J].Northern Horticulture,2019,43(08):73-81.[doi:10.11937/bfyy.20183217]
钒、钛、镉单一和联合胁迫对地钱生理生化的影响
- Title:
- Single and Combined Effects of Vanadium,Titanium and Cadmium on the Physiology and Biochemistry of Marchantia polymorpha L.
- Keywords:
- Marchantia polymorpha; vanadium (V); titanium (Ti) and cadmium (Cd); stress; physiological index
- 文献标志码:
- A
- 摘要:
- 以地钱（Marchantia polymorpha L.）为试材，采用喷洒金属溶液的方法，研究了0~20 d内稀有金属钒（V）和钛（Ti）单一胁迫以及V、Ti、镉（Cd）联合胁迫方式下地钱对3种金属的富集能力和生理指标的影响，以期探究地钱对V、Ti单一胁迫以及V-Ti-Cd联合胁迫的耐受性及其响应机理。结果表明：V、Ti单独胁迫地钱时，地钱对V的富集程度为(808.00±26.46)μg?g-1大于对Ti（766.67±62.31)μg?g-1的富集，2种金属胁迫下地钱生理指标变化趋势相同，0~20 d的胁迫时间内，可溶性蛋白质含量减少，可溶性糖含量增加，脯氨酸（Pro）含量减少，丙二醛（MDA）含量先增加后减少在12 d时最高，超氧化物歧化酶（SOD）活性先升高再降低再升高，过氧化氢酶（CAT）和过氧化物酶（POD）活性整体均增强，第20天时最强。V-Ti-Cd联合胁迫时，地钱对Cd的富集能力最强，达到(1 179.00±103.12)μg?g-1，而V、Ti的积累比单独胁迫时分别降低(243.00±27.87)μg?g-1和(349.33±55.05)μg?g-1，联合胁迫对V、Ti的积累表现出拮抗作用。Pro含量增加到12 d时最高后下降再升高，整体增加，而MDA含量增加，在第20天时含量最高，SOD活性增强以及CAT活性先增加后降低再升高，均在第20天达到最高，POD活性在第4天增加后降低并稳定。相比单独胁迫，短时间内复合胁迫对地钱产生的毒害作用更大。该结果为植物对多元金属复合胁迫的生理响应及耐受机制研究提供了一定的理论基础，也为富含钒钛磁铁矿地区的植被恢复提供新的途径。
- Abstract:
- In this study,Marchantia polymorpha L.were dealt with independent vanadium (V) and titanium (Ti) and joint V,Ti and cadmium (Cd) by spraying metal solution under different time gradient.In order to explore the tolerance of M.polymorpha to single stress of V,Ti and V-Ti-Cd combined stress and its response mechanism.The results showed that the contents of V (808.00±26.46)μg?g-1 was higher than Ti (766.67±62.31)μg?g-1 in M.polymorpha and the physiological index changes was similar,when M.polymorpha was treated with single V or Ti.Compared with the control group,it appeared that soluble protein contents were reduced,soluble sugar contents were increased,the contents of proline (Pro) were decreased,malondialdehyde (MDA) contents were increased at first then cut down and were reached the top on the 12th day,superoxide dismutase (SOD) activity was increased at first then decreased and was increased again,catalase (CAT) activity and peroxidase (POD) activity was increased to the top on the 20th day.Furthermore,when M.polymorpha was threatened by V-Ti-Cd simultaneously,the maximum contents of Cd (1 179.00±103.12)μg?g-1 and the contents of V (243.00±27.87)μg?g-1 and Ti (349.33±55.05)μg?g-1 were restrained significantly.Pro contents were increased to the top on the 12th day then were decreased and increased again.MDA contents were increased,SOD activity was enhanced,CAT was activity enhanced at first then was decreased and enhanced again.POD activity was enhanced on the 4th day then reduced and stabilized.Compared with the single stress,the combined stress had more toxic effect on the M.polymorpha in a short time.These results could provide new ways for vegetation recovery in vanadium titano-magnetite region,as well as provide a theoretical basis for studying the tolerance of plants for multiple metal stresses.
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备注/Memo
第一作者简介：杜泽萱（1995-），女，硕士研究生，研究方向为苔藓植物资源保护及利用。E-mail：kkxndzx@163.com.责任作者：王丽（1969-），女，博士，教授，现主要从事资源植物学等研究工作。E-mail：yzxj@vip.163.com.基金项目：四川省科技厅重点研发资助项目（2017SZ0186）。收稿日期：2018-12-03