LI Shisheng,ZHENG Zijun,ZENGCHEN Qiuyu,et al.Establishment of Male Sterile Line in Huangzhou Radish Based on Chemical Mutagenesis[J].Northern Horticulture,2019,43(08):58-62.[doi:10.11937/bfyy.20182819]
EMS诱变获得黄州萝卜雄性不育材料
- Title:
- Establishment of Male Sterile Line in Huangzhou Radish Based on Chemical Mutagenesis
- Keywords:
- Huangzhou radish; mutagenesis; male sterile; molecular marker
- 文献标志码:
- A
- 摘要:
- 以黄州萝卜为试材，采用对不同浓度EMS（0、0.2%、0.4%、0.6%、0.8%、1.0%、2.0%(V/V)）浸种对萝卜种子处理的方法,研究了EMS对黄州萝卜的诱变效应。以期为黄州萝卜不育材料的获得及萝卜雄性不育系选育与利用奠定良好基础。结果表明：随着EMS浓度的增加(从0.2%增加到2.0%)，黄州萝卜种子萌发与根生长受到抑制，表明EMS对黄州萝卜种子萌发及后期根的生长具有抑制作用。根据50%致死浓度的选择标准，黄州萝卜适宜的EMS浓度为0.4%~0.8%。最终以0.5%EMS处理黄州萝卜种子。与对照相比较，处理组除表现主根增粗外，在黄州萝卜诱变群体中发现其雄性不育且均为100%不育。花药形态学观察与切片结果显示花药明显褶皱,卷曲及褐化，最终于花粉四分体时期药室壁结构崩解所致花粉败育。萝卜雄性不育分子标记ORF138-F/R检测结果也证实在黄州萝卜雄性败育材料中能扩增到相应大小条带，而在对应保持系中始终未扩增出条带。
- Abstract:
- The mutagenic effect of EMS on the seeds of radish was studied by soaking seeds with different concentration of EMS（0，0.2%，0.4%，0.6%，0.8%，1.0%，2.0%(V/V)）.In order to establish a good foundation for the breeding and utilization of male sterile line of radish by obtaining the sterile material of Huangzhou radish.The results showed that in the aspect of germination rate and root length,the growth of radish was inhibited with the increase of EMS concentration (from 0.2% to 2.0%).So the inhibition of EMS to Huangzhou radish was obvious.According to the selection criteria of 50% lethal concentrations,the suitable EMS concentration of Huangzhou radish was 0.4%-0.8%.The seed of Huangzhou radish was treated with 0.5% EMS.It was found that the main agronomic characters of the treatment group were thickened by compared with control group.Furthermore,the male sterility in the Huangzhou radish mutation group was found and it was very stable.The results showed that the anther was obviously wrinkled,curled and brown,and eventually the pollen abortion was caused by disintegration of the inter wall of pollen by morphological and cytological observation.It also showed that it could be enlarged to the corresponding size bands in the male sterile line of Huangzhou radish,while the band was not amplified in normal line by detection of molecular marker ORF138-F/R in radish.
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[J].Northern Horticulture,2019,43(08):198.[doi:10.11937/bfyy.20182676]
备注/Memo
第一作者简介：李世升（1983-），男，博士，副教授，研究方向为作物育种。E-mail:21581872@qq.com.基金项目：湖北省中央引导地方科技发展专项资助项目（2018ZYYD019）。收稿日期：2018-08-25