[1]陈婉婷,倪必勇,张富强,等.基于ITS序列的栽培杏种质亲缘关系及政和杏的分类研究[J].北方园艺,2019,43(07):27-33.[doi:10.11937/bfyy.20182702]
　CHEN Wanting,NI Biyong,ZHANG Fuqiang,et al.Genetic Relationships of Cultivation Apricot and Classification of Prunus zhengheensis Revealed by ITS Sequences[J].Northern Horticulture,2019,43(07):27-33.[doi:10.11937/bfyy.20182702]

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基于ITS序列的栽培杏种质亲缘关系及政和杏的分类研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷期数: 2019年07 页码: 27-33 栏目: 出版日期: 2019-04-15

Title:: Genetic Relationships of Cultivation Apricot and Classification of Prunus zhengheensis Revealed by ITS Sequences

作者:: 陈婉婷1; 倪必勇2; 张富强3; 李明1; 郑品光2; （1.福建农林大学林学院，福建福州 350002；2.福州植物园，福建福州 350002；3.福建省政和县林业局，福建南平 353600）

Author(s):: CHEN Wanting1; NI Biyong2; ZHANG Fuqiang3; LI Ming1; ZHENG Pinguang2; (1.Forestry College,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002；2.Fuzhou National Forest Park,Fuzhou,Fujian 350002；3.Zhenghe Forestry Bureau,Nanping,Fujian 353600)

关键词:: 栽培杏; 政和杏; 亲缘关系; ITS序列

Keywords:: cultivated apricot; Prunus zhengheensis; genetic relationship; ITS sequence

DOI:: 10.11937/bfyy.20182702

文献标志码:: A

摘要:: 以46个主要栽培杏和野生杏种质为试材，采用对杏种质资源ITS序列的克隆和测序方法，研究了主要栽培杏种质的亲缘关系和政和杏的分类地位，以期为杏种质资源分类和遗传育种提供参考依据。结果表明：杏种质资源具有较高的遗传多样性，中性检验显示出显著的负向选择进化模式。基于ITS序列的系统发育树表明，基于ITS序列可较好地将杏组植物划分为梅、政和杏、西伯利亚杏、普通杏和杏李5组，仁用杏与西伯利亚杏的亲缘关系更为相近。相比于普通杏，政和杏与梅的亲缘关系更为相近，应当为梅的一个变种。

Abstract:: The genetic relationships of cultivation apricot and the phylogenetic classification of Prunus zhengheensis were studied based on amplification,sequencing and phylogenetic analysis of the internal transcribed spacer(ITS) regions of nuclear ribosomal DNA for 46 main cultivated apricots accessions,in order to supply references for Apricot germplasm resources classification and genetic breeding.The results showed thatt apricot accessions had high levels of genetic diversity and a negative selection evolution model with highly significant and negative values in statistical neutrality tests.Based on the ITS sequences,the phylogenetic tree gave five clear grouping,Prunus mume,〖JP2〗Prunus zhengheensis,Prunus sibirica,Prunus armeniaca and Prunus simonii.And the results supported a close relationship between kernelled apricot and Prunus sibirica.Compared with Prunus armeniaca,Prunus zhengheensis showed a closer relationship with Prunus mume,which suggested that it should be a variant of Prunus mume.

参考文献/References:

[1]ZHEBENTYAYEVA T,LEDBETTER C,BURGOS L,et al.Apricot[M]//In:BADENES M,BYRNE D (eds).Fruit breeding.Boston:Springer,2012:415-458.[2]张加延,张钊.中国果树志?杏卷[M].北京:中国林业出版社,2003.[3]LI M,ZHAO Z,MIAO X.Genetic diversity and relationships of apricot cultivars in North China revealed by ISSR and SRAP markers[J].Scientia Horticulturae,2014,173:20-28.[4]LI M,ZHAO Z,MIAO X,et al.Genetic diversity and population structure of Siberian apricot (Prunus sibirica L.) in China[J].International Journal of Molecular Sciences,2013,15(1):377-400.[5]张加延,吕亩南.杏属二新种[J].Journal of Systematicss & Sevolution,1999,37(1):105-109.[6]BOUHADIDA M,MARTN J P,EREMIN G,et al.Chloroplast DNA diversity in Prunus and its implication on genetic relationships[J].Journal of the American Society for Horticultural Science,2007,132(5):670-679.[7]BORTIRI E,OHS H,JIANG J,et al.Phylogeny and systematics of Prunus（Rosaceae） as determined by sequence analysis of ITS and the chloroplast trnL-trnF spacer DNA[J].Systematic Botany,2001,26(4):797-807.[8]章秋平,魏潇,刘威生,等.基于叶绿体DNA序列trnL-F分析李亚属植物的系统发育关系[J].果树学报,2017,34(10):1249-1257.[9]刘艳玲,徐立铭,程中平.基于ITS序列探讨核果类果树桃、李、杏、梅、樱的系统发育关系[J].园艺学报,2007,34(1):23-28.[10]ZHENG D S,YANG Q W.Genetic resources of wild relatives of crops in China[J].Journal of Plant Genetic Resources,2014,15(1):1-11.[11]王家琼,吴保欢,崔大方,等.基于30个形态性状的中国杏属(Armeniaca Scop.)植物分类学研究[J].植物资源与环境学报,2016,25(3):103-111.[12]王家琼.中国杏属植物的分类、分布及其亲缘关系研究[D].广州:华南农业大学,2010.[13]章秋平,刘威生,刘宁,等.基于形态性状的仁用杏种质资源分类研究[J].果树学报,2015(3):385-392.[14]WEI Y J,LI W W,FENG B B,et al.Study on the flowering biological characteristics of Prunus domestica×armeniaca[J].Agricultural Science & Technology,2016,18(3):577-583.[15]YANG,H W,PAN D.Identification of hybrids between Prunus salicina and Prunus armeniaca by RAPD and SSR[J].Journal of Anhui Agricultural Sciences,2012(6):15-17.[16]包文泉.基于形态、叶绿体基因组及核基因组SSR的仁用杏分类地位研究[D].北京:中国林业科学,2017.[17]包文泉,乌云塔娜,王淋,等.野生杏和栽培杏的遗传多样性和遗传结构分析[J].植物遗传资源学报,2017,18(2):201-209.[18]韩志校,张军,左力辉,等.基于SSR和ITS标记的杨树多态性及聚类分析[J].中国农业科技导报,2017,19(7):36-43.[19]BATNINI M A,BOURGUIBA H,TRIFI-FARAH N,et al.Chloroplast DNA sequence data provides new insights into genetic diversity and phylogenetic relationships of Tunisian apricot germplasm[J].Scientia Horticulturae,2014,178:241-247.[20]宋春凤,吴宝成,周伟,等.基于psbA-trnH序列变异分析川明参属亲缘关系及分类地位[J].植物资源与环境学报,2014(2):19-26.[21]栗丹,李振坚,毛萍,等.基于ITS序列石斛材料的鉴定及系统进化分析[J].园艺学报,2012,39(8):1539-1550.[22]王利兵.我国3种杏的地理分布及其植物学性状[J].林业科学研究,2010,23(3):435-439.[23]HE T M,CHEN X S,XU Z,et al.Using SSR markers to determine the population genetic structure of wild apricot (Prunus armeniaca L.) in the Ily Valley of West China[J].Genetic Resources and Crop Evolution,2007,54(3):563-572.[24]ZHEBENTYAYEVA T N,REIGHARD G L,LALLI D,et al.Origin of resistance to plum pox virus in Apricot:What new AFLP and targeted SSR data analyses tell[J].Tree Genetics & Genomes,2008,4(3):403-417.[25]廖明康,郭丽霞,张平,等.新疆杏属植物过氧化物酶儿茶酚氧化酶同工酶分析[J].西北农业学报,1994,3(2):81-86.[26]孙浩元,杨丽,张俊环,等.杏种质资源研究进展[J].经济林研究,2017,35(3):251-258.

备注/Memo

第一作者简介：陈婉婷（1992-），女，硕士研究生，研究方向为林木遗传育种。E-mail：cwting2004@gmail.com.责任作者：郑品光（1963-），男，本科，高级工程师，现主要从事林业及植物资源等研究工作。E-mail：zpgfzfa@163.com.基金项目：福建省林业专项资金资助项目（闽财指2015-689）；中国科学院科技服务网络计划资助项目（KFJ-3W-No.1）。收稿日期：2018-10-25

更新日期/Last Update: 2019-04-30