MAOJing,DONGYanfang,ZHOUYuan,et al.PhysiologicalCharacteristicsofDifferentIrisgermanicaCultivarstoHighTemperatureStressandEvaluationofTheirHeatTolerance[J].Northern Horticulture,2019,43(03):94-100.[doi:10.11937/bfyy.20182416]
十六个德国鸢尾品种的耐热性评价
- Title:
- PhysiologicalCharacteristicsofDifferentIrisgermanicaCultivarstoHighTemperatureStressandEvaluationofTheirHeatTolerance
- 文章编号:
- 20182416
- Keywords:
- Keywords:Irisgermanica; evaluationofheattolerance; hightemperaturestress; physiologicalcharacteristic
- 文献标志码:
- A
- 摘要:
- 摘要：以16个德国鸢尾品种叶片为试材，在高温条件下测定叶片中叶绿素、丙二醛（MDA）、脯氨酸和可溶性蛋白质含量及超氧化物歧化酶（SOD）和过氧化物酶（POD）活性，利用主成分、隶属函数及聚类分析方法对测定指标进行评价排序，综合鉴定比较16个德国鸢尾品种的耐热特性，以期为研究和利用德国鸢尾耐热品种提供参考依据。结果表明：不同品种在常温下的各项生理指标以及高温胁迫后的变化幅度各不相同；高温胁迫下，德国鸢尾各个品种的叶片中叶绿素含量均有不同程度降低；SOD与POD活性增强；MDA、脯氨酸及可溶性蛋白质含量升高；结合主成分分析、隶属函数值排序，16个德国鸢尾品种间耐热性强弱依次为“魂断蓝桥”“PartyDress”“Galamadrid”“音箱”“黑骑士”“Ninbeiunge”“Queen”“Jessica”“不朽白”“法国回声”“金娃娃”“笛声”“洋娃娃”“黄莲”“皇家十字军”“短梦”。通过检测高温胁迫下德国鸢尾叶片生理指标，并利用主成分和隶属函数分析建立德国鸢尾耐热性的综合鉴定与评价体系，适用于耐热型德国鸢尾品种资源的评价与筛选。
- Abstract:
- Abstract:Thephysiologicalindexwasdetectedandanalyzedinordertoprovidereferencesforevaluatingandscreeningheat-tolerantcultivarsofIrisgermanica.Underhigh-temperaturestress，thephysiologicalindicesincludingthecontentsofchlorophyll，malondialdehyde，solubleproteinandproline，andactivitiesofantioxidantwasdetermined.Thentheheat-tolerancesof16cultivarswererankedbasedonprincipalcomponentsanalysisandsubordinatefunction.Theresultsshowedthatdifferentcultivarsshowedvariedlevelsofphysiologicalindices.Thephysiologicaldetectionshowedthatchlorophyllcontentswerereducedineachcultivar，andactivitiesofSODandPODwereincreased，whilethecontentsofMDA，prolineandsolubleproteinwerehigherafterthehightemperaturetreatment.CombinedwithPCAandsubordinatefunctionanalysis，heattoleranceof16cultivarswererankedas‘HDLQ’‘PartyDress’‘Galamadrid’‘YX’‘HQS’‘Ninbeiunge’‘Queen’‘Jessica’‘BXB’‘FGHS’‘JWW’‘DS’‘YWW’‘HL’‘Royal’‘DM’.TheestablishedevaluationmethodbasedonPCAandsubordinatefunctionwasfeasibleforevaluatingandselectingheat-tolerantcultivarsofIrisgermanica.
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备注/Memo
第一作者简介：毛静(1980-)，女，博士，高级工程师，现主要从事观赏植物抗逆性评价与代谢组学等研究工作。E-mail：jeanmao2008@163.com.基金项目：武汉市科技局宿根花卉工程技术研究中心资助项目（武科计[2016]42）。收稿日期：2018-10-08