XU Liangzheng,LI Nuo,HE Guiling,et al.Experiment and Analysis of Deficiency Symptoms of Calcium, Magnesium and Sulfur of Solanum torvum Seedlings[J].Northern Horticulture,2019,43(04):1-6.[doi:10.11937/bfyy.20182037]
水茄幼苗钙、镁、硫缺素症的试验分析
- Title:
- Experiment and Analysis of Deficiency Symptoms of Calcium, Magnesium and Sulfur of Solanum torvum Seedlings
- Keywords:
- Solanum torvum; solution culture; nutrient deficiency symptoms; calcium; magnesium; sulfur
- 文献标志码:
- A
- 摘要:
- 以野生水茄幼苗为试材，在室内溶液培养条件下，诱导水茄Ca、Mg、S的缺素症，分析各缺素处理下水茄的生长和生理差异，以期为水茄幼苗规模化生产中营养缺素诊断提供依据。结果表明：各缺素处理均诱导水茄出现能明显察觉和区分的专一缺素症；不同缺素诱导水茄出现相应缺素症的时间和程度有差别，3种缺素症以缺Ca症表现最早和最明显；不同缺素处理均影响水茄幼苗生长，缺素处理的叶片数、全株干物质质量、根冠比、总根长、根总体积、根系活力均显著低于完全培养。其中，缺Ca和缺S水茄叶片数最少，分别比对照和缺Mg水茄少了16.33%和6.81%。缺Ca水茄全株干物质质量、总根长、根总体积、根系活力均最低，分别比其它营养状况下的低了11.30%～42.70%、27.75%～41.88%、14.29%～66.67%、20.98%～75.01%。缺Mg植株根冠比和老叶叶绿素含量均最低，分别比对照、缺Ca和缺S水茄低了29.41%、14.29%、45.46%与75.00%、60.00%、55.56%。嫩叶叶绿素含量以缺Ca的最低，分别比对照和缺Mg、缺S的低42.31%、25.00%和16.67%。对照根系活力分别是Ca、Mg、S缺素植株的4.00、2.03、3.16倍。
- Abstract:
- Deficiency symptoms of calcium，magnesium and sulfur in the Solanum torvum seedlings cultured in nutrient solution were induced and observed，along with the determination of growth parameters and physiological characteristics.The conclusions of the present study provided a valuable basis for nutrient deficiency diagnosis in the large scale production of Solanum torvum seedlings.The results indicated that each treatment of a specific nutrient deficiency induced exclusive symptoms；the emergence time and severity of three different nutrient deficiencies were different，with symptoms of calcium deficiency emerging the earliest and the most obvious；all the three nutrient deficiencies impacted adversely the growth of Solanum torvum seedlings，namely the number of leaves，whole-plant dry weight and root volume of the deficiency treatments were all significantly lower than those of the non-deficiency control.Calcium deficiency and sulfur deficiency resulted in significant lesser leaf numbers than those of the control and magnesium deficiency by 16.33% and 6.81%，respectively.Calcium deficiency led to the lowest whole-plant dry weight，total root length，total root volume and root activity，namely lower than other treatments by 11.30%-42.70%，27.75%-41.88%，14.29%-66.67%，20.98%-75.01%，respectively.Magnesium deficiency led to the lowest root to shoot ratio and chlorophyll content in old leaves，lower than the control，calcium deficiency and sulfur deficiency by 29.41%，14.29%，45.46% and 75.00%，60.00%，55.56%.Calcium deficiency led to the lowest chlorophyll content in tender leaves，lower than the control，magnesium deficiency and sulfur deficiency by 42.31%，25.00% and 16.67%.The root activity of the control was 4.00，2.03 and 3.16 times of those of calcium，magnesium and sulfur deficiencies.
参考文献/References:
[1]林桂荣,周宝利.茄砧托鲁巴姆(Solanum torvum)种子休眠与萌发习性研究[J].种子,2005,24(5):38-40.[2]高梅秀,孙世海.野生茄子繁种试验研究[J].北方园艺,2003(4):50-51.[3]王贵余.野生茄“托鲁巴姆”保护地繁种技术[J].北方园艺,2004(1):21.[4]李楠洋,陈钰辉,刘富中,等.托鲁巴姆（Solanum torvum Sw.）在茄子嫁接栽培上的应用研究[J].中国蔬菜,2013(10):1-8.[5]岳宏忠,郭兰香,侯栋.茄子砧木托鲁巴姆发芽试验研究[J].甘肃农业科技,2002(8)：11-12．[6]张红.茄子砧木“托鲁巴姆”组培快繁简化技术[J].北方园艺,2011(17):146-148．[7]罗宏伟,张英樊,戴耀,等．三亚托鲁巴姆茄子嫁接苗病株病原菌分子鉴定及室内药效测定[J].海南热带海洋学院学报,2018,25(2):84-88．[8]中国科学院中国植物志编辑委员会.中国植物志(第六十七卷第一分册)[M].北京:科学出版社,1978：95.[9]水茄[EB/OL].http://www.zhiwutong.com/latin/Solanaceae/Solanum-torvum-Swartz.htm.[10]苏婉玉,王艳芳,曹绍玉,等.野生茄属资源：水茄的开发利用[J].长江蔬菜,2017(22):32-34.[11]李显仁,林雄武.茄子与野生水茄嫁接及其嫁接苗的栽培技术[J].广西园艺,2008,19(1):54-55.[12]胡永军,李明光,陈永智,等.茄子嫁接栽培防治根结线虫砧木的筛选[J].长江蔬菜,2004(6):32-33.[13]魏小伞,曹必好,雷建军,等.茄子抗病育种研究进展[J].中国蔬菜,2010(10):1-8.[14]JUE D W，YANG l，SHI C,et al.Cloning and characterization of a Solanum torvum 〖STBX〗NPR1 〖STBZ〗gene involved in regulating plant resistance to Verticillium dahliae[J].Acta Physiol Plant,2014(36):2999-3011.[15]许良政,罗来辉,李坤新,等.野生蔬菜水茄种子发芽特性的研究[J].种子,2009,28(8):45-47.[16]房志坚,田素英,姚广杰.水茄的繁殖方法研究[J].现代中药研究与实践,2011,25(3):12-14.[17]WANG Y K，LI W J，JIANG Y Q,et al.Establishment of rapid propagation technique for cotyled on of Solanum torvum[J].Agricultural Science & Technology,2015,16(10):2167-2169.[18]方岩岩.水茄植株再生及其与紫长茄杂交F1代花药培养的研究[D].南宁：广西大学,2013.[19]王红.栽培茄自交系（116、117）与水茄远缘杂交F1杂种鉴定及种质的抗病性分析[D].广西大学,2014.[20]刘炎霖,陈钰辉,刘富中,等.水茄泛素结合酶E2基因StUBCc的克隆及黄萎病菌诱导表达分析[J].园艺学报,2015,42(6):1185-1194.[21]李小芳,张志良.植物生理学实验指导[M].5版.北京:高等教育出版社,2016：19-21.[22]史芝文,高继国,郝冉彬.植物生理生化实验技术[M].哈尔滨:东北农业大学出版社,1993:20-78.[23]陈福明,陈顺伟.混合液法测定叶绿素含量的研究[J].林业科技通讯,1984(2):4-8.[24]夏金川,郭秀珠,黄品湖.不同TTC法测定枫香等阔叶树容器苗根系活力探讨[J].浙江农业科学,2008(1):39-42.[25]李春俭.高级植物营养学[M].北京:中国农业大学出版社,2008:159-261.[26]毛伟海,包崇来,胡齐赞,等.茄子的栽培生理与高效丰产技术[M].北京:中国农业出版社,2003：286-288.[27]高桥英一,吉野实,前田正男，著.张美善,译.植物营养元素缺乏与过剩诊断[M].长春:吉林科学技术出版社,2002：15-31.[28]许良政,李威娜,廖仲英.琯溪蜜柚幼苗几种缺素症的诱导与研究[J].嘉应学院学报(自然科学版),2012,30(2):48-52.[29]黄高峰,钟启文,王丽慧,等.菊芋苗期缺钾症状及生理特性研究[J].北方园艺,2011(6):37-39.
备注/Memo
第一作者简介：许良政(1965-)，男，湖北阳新人，本科，教授，现主要从事植物营养生理及驯化生理学等研究工作。E-mail:xuliangzhengjyu@126.com.基金项目：中央财政支持地方高校发展专项资助项目（财教[2015]196号，粤财教[2015]304号）；广东省科技计划资助项目（2012A020602067）；广东省本科高校高等教育综合类改革资助项目（粤教高函[2016]236号）；广东省本科高校教学改革与教学质量工程建设资助项目（粤教高函[2015]133号）；梅州市科学技术局-嘉应学院联合自然科学研究资助项目（2012KJM04）。收稿日期：2018-08-06