XIN Dongdong,LI Guirong,GUO Weili,et al.Expression Analysis of MAPK Gene of Muskmelon[J].Northern Horticulture,2018,42(24):21-26.[doi:10.11937/bfyy.20181707]
甜瓜MAPK基因的表达分析
- Title:
- Expression Analysis of MAPK Gene of Muskmelon
- Keywords:
- melon; melon powdery mildew; MAPK gene; expression analysis
- 文献标志码:
- A
- 摘要:
- 以“哈密加格达”‘Edisto47’甜瓜为试材，采用实时定量PCR（qRT-PCR）方法，研究了水杨酸（SA）、乙烯利（ETH）和南瓜白粉病病原菌处理甜瓜叶片的MAPK基因的表达情况，以期为甜瓜MAPK基因的调控功能研究及甜瓜抗白粉病育种提供参考依据。结果表明：SA处理抗病甜瓜叶片9 h后的[STBX]MAPK9[STBZ]、[STBX]MAPK21[STBZ] 2个基因的表达上调，[STBX]MAPK21[STBZ]基因表达量最大，SA处理感病甜瓜叶片3、24、72 h后的[STBX]MAPK21[STBZ]表达量上调；ETH处理抗病甜瓜叶片6 h后[STBX]MAPK9、MAPK21[STBZ] 2个基因的表达量上调明显，[STBX]MAPK9[STBZ]基因表达量最大，ETH处理感病甜瓜叶片48 h后[STBX]MAPK21[STBZ]基因的表达量最大；南瓜白粉病孢子处理抗甜瓜叶片12 h后[STBX]MAPK9[STBZ]基因的表达量上调最明显；南瓜白粉病孢子处理感病甜瓜叶片24 h后[STBX]MAPK9[STBZ]基因的表达量上调明显，综上所述，在甜瓜叶片中SA、ETH、南瓜白粉病病原菌均可以诱导该基因的表达。其中，[STBX]MAPK9、MAPK21[STBZ]分别在SA处理抗病甜瓜叶片9 h后和SA处理感病甜瓜叶片3、24、72 h后能够响应SA的诱导；[STBX]MAPK9[STBZ]能够响应ETH和南瓜白粉病孢子的诱导。
- Abstract:
- ‘Hami Jiageda’ and ‘Edisto47’ melon were used as experimental materials,by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) method to study the expression of the MAPK genes,screening of melon powdery mildew resistance genes and analyses the expression of resistance genes,in order to provide references for regulatory function of the melon MAPK genes and the breeding of melon against powdery mildew.The results showed that the expression of MAPK9and MAPK21 [STBZ]was up-regulated after SA treatment for 9 hours of disease-resistant melon leaves,and the expression of MAPK21gene was the largest,the expression of MAPK21gene was up-regulated after SA treatment for 3 hours,24 hours and 72 hours of susceptible melon leaves.The expression levels of MAPK9 [STBZ]and MAPK21were significantly up-regulated after ETH treated the disease-resistant melon leaves for 6 hours.The expression of MAPK21gene was the greatest after ETH treated the susceptible melon leaves for 48 hours.The expression of MAPK9gene was significantly up-regulated after pumpkin powdery mildew spore treatment for 12 hours of disease-resistant melon leaves.The expression level of MAPK9 [STBZ]gene was significantly up-regulated after pumpkin powdery mildew spore treatment for 24 hours of susceptible melon leaves.To sum up,SA,ETH and pumpkin powdery mildew pathogen could induce the expression of this gene in melon leaves.Among them,both MAPK9 and MAPK21 were able to respond to SA induction after SA treatment for 9 hours of disease-resistant melon leaves and SA treatment for susceptible leaves for 3 hours,24 hours,72 hours,respectively.MAPK9was able to respond to the induction of in ETH and pumpkin powdery mildew spores.
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备注/Memo
第一作者简介：辛董董（1994-），男，硕士研究生，研究方向为园艺植物生理特性及生物技术育种。E-mail：18703735217@163.com.责任作者：李桂荣（1974-），女，博士，副教授，现主要从事园艺植物育种与生物技术教学与科研等工作。E-mail：liguirong10@163.com.基金项目：国家自然科学基金资助项目（U1404321）；河南省自然基金资助项目（182300410031）；河南省高校重点科研资助项目（16A21003）。收稿日期：2018-07-03