[1]宋慕波,帅良,李淋,等.马蹄查耳酮合酶基因的克隆及其在鲜切马蹄中的表达分析[J].北方园艺,2018,42(22):11-18.[doi:10.11937/bfyy.20181559]
　SONG Mubo,SHUAI Liang,LI Lin,et al.Cloning and Expression Analysis of CHS Gene in Freshcut Chinese Waterchestnut[J].Northern Horticulture,2018,42(22):11-18.[doi:10.11937/bfyy.20181559]
点击复制

马蹄查耳酮合酶基因的克隆及其在鲜切马蹄中的表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年22 页码: 11-18 栏目: 出版日期: 2018-11-25
Title:
Cloning and Expression Analysis of CHS Gene in Freshcut Chinese Waterchestnut
作者:
宋慕波1; 2; 帅良1; 2; 李淋1; 方方1; 2; 陈振林1; 2; 段振华1; 2
（1.贺州学院 食品科学与工程技术研究院，广西 贺州 542899；2.广西果蔬保鲜和深加工人才小高地，广西 贺州 542899）
Author(s):
SONG Mubo1; 2; SHUAI Liang1; 2; LI Lin1; FANG Fang1; 2; CHEN Zhenlin1; 2; DUAN Zhenhua1; 2
(1.Institute of Food Science and Engineering Technology,Hezhou University,Hezhou,Guangxi 542899;2.Guangxi Talent Highland of Preservation and Deep Processing Research in Fruit and Vegetables,Hezhou,Guangxi 542899)
关键词:
鲜切马蹄; 查尔酮合酶; 基因克隆; 表达分析
Keywords:
freshcut Chinese waterchestnut; chalcone synthase; gene cloning; expression analysis
DOI:
10.11937/bfyy.20181559
文献标志码:
A
摘要:
以鲜切马蹄为试材，利用RTPCR和RACE技术克隆马蹄CHS基因全长序列，并对该基因进行生物信息学分析及表达分析，以期为该基因的功能研究提供参考依据。结果表明：在马蹄中克隆到CHS全长cDNA序列，基因命名为CwCHS。CwCHS基因全长1 519 bp，编码394个氨基酸。CwCHS 蛋白分子式为C.1 927H.3 072N.526O.568S.21，分子量为43.37 kDa。该蛋白质包含3个保守结构域，含有5个酪蛋白激酶Ⅱ识别位点、1个蛋白激酶C识别位点、6个N豆蔻酰化位点和1个糖基化位点，其二级结构以α螺旋为主。多重比对结果表明，CwCHS具有高度保守的酶活性位点，并与其它植物的CHS蛋白有很高的同源性。系统进化分析表明CwCHS与菠萝CHS蛋白亲缘关系较近。荧光定量PCR分析结果表明鲜切马蹄贮藏过程中CwCHS表达量快速上升，水杨酸处理显著抑制了CwCHS的表达。试验发现，CwCHS的表达水平与鲜切马蹄黄化关系密切。
Abstract:
Freshcut Chinese waterchestnut was used as material,the full cDNA sequence of CHS was obtained by using the RTPCR and RACE methods，the bioinformatics and expression of CHS were analyzed in order to supply reference for gene function study.The results showed that the cDNA sequence of CHS was obtained,and it was named CwCHS.CwCHS consisted of 1 519 bp,and encoding a polypeptide of 394 amino acids.The formula of CwCHS was C.1 927H.3 072N.526O.568S.21,molecular weight was 43.37 kDa.CwCHS contained three conserved domains.Besides,CwCHS included casein kinase II phosphorylation sites for five,protein kinase C phosphorylation sites for one,Nmyristoylation site for six and Nglycosylation site for one.Alpha helix was the maximum structural part in the protein secondary structure of CwCHS.The multiple sequence alignment showed that CwCHS had the highly conservative enzyme activity sites,and was highly homologous to other CHS proteins from different plants.Phylogenetic tree analysis showed that CwCHS was very closely related to CHS protein of Ananas comosus.The analysis of realtime PCR showed that the expression level of CwCHS in freshcut Chinese waterchestnut increased dramatically,however,salicylic acid treatment significantly inhibited the expression of CwCHS.These results suggested that expression of CwCHS closely related to surface etiolation phenomenon of freshcut Chinese waterchestnut.

相似文献/References:

[1]黎霜,冉佳鑫,李孝绒,等.中国樱桃‘玛瑙红’CpCHS1基因的克隆与表达分析[J].北方园艺,2022,(13):16.[doi:10.11937/bfyy.20220028]
　LI Shuang,RAN Jiaxin,LI Xiaorong,et al.Cloning and Expression Analysis of CpCHS1 Gene of Cerasus pseudoceresus ‘Manaohong’[J].Northern Horticulture,2022,(22):16.[doi:10.11937/bfyy.20220028]
[2]何金娇,张万方,侯玥如,等.茄子查尔酮合酶基因的克隆与生物信息学分析[J].北方园艺,2023,(19):16.[doi:10.11937/bfyy.20230436]
　HE Jinjiao,ZHANG Wanfang,HOU Yueru,et al.Cloning and Bioinformatics Analysis of Chalcone Synthase Gene in Eggplant[J].Northern Horticulture,2023,(22):16.[doi:10.11937/bfyy.20230436]

备注/Memo

第一作者简介：宋慕波（1986），男，博士，副研究员，研究方向为果蔬保鲜。Email:songmubo1@163.com.责任作者：段振华（1965），男，博士，教授，研究方向为果蔬加工与保鲜。Email:dzh65@163.com.基金项目：国家自然科学基金资助项目（31801607）；广西自治区自然科学基金资助项目（2017GXNSFAA198082，2015GXNSFBA139082）；广西特聘专家专项经费资助项目（厅发[2016]21号）。收稿日期：2018-06-27

更新日期/Last Update: 2018-12-03