ZONG Shubin,WANG Yongping,GU Lixin,et al.Efficient Multiplication Technology of Vaccinium corymbosum Plantlets in New Type Bioreactors System[J].Northern Horticulture,2018,42(24):59-64.[doi:10.11937/bfyy.20180924]
蓝莓组培苗在新型生物反应器中的高效增殖技术
- Title:
- Efficient Multiplication Technology of Vaccinium corymbosum Plantlets in New Type Bioreactors System
- Keywords:
- Vaccinium corymbosum; temporary immerion; plantlet
- 文献标志码:
- A
- 摘要:
- 以蓝莓第3代组培苗为试材，采用对培养液体积、摇摆角度、接种密度3因素3水平L9(34)正交实验和对摇摆频率、浸没时间二因素完全随机试验方法，研究了蓝莓组培苗在新型摆动式间歇浸没生物反应器（S-TIBs）中的高效增殖技术，以期建立蓝莓组培苗在新型生物反应器中增殖培养的技术体系。结果表明：培养液体积每瓶250 mL，摇摆角度45°，接种密度每瓶60株为最佳的培养参数，摇摆频率因素最佳为单次6 h，浸没时间设定为60 s。
- Abstract:
- The third generation of blueberry plantlet was used as test material.The efficient multiplication technology of blueberry plantlets in S-TIBs was systematically studied through L9(34) orthogonal experiment with three factors and three levels (culture solution volume,swing angle and inoculation density) and completely randomized experiment with two factors (swing frequency and immersion time).The results showed that the optimal culture parameters were set as follows,the culture medium volume of 250 mL each bottle,the swing angle at 45°,and the inoculation density of 60 plantlets each bottle.The optimal swing frequency was 6 hours each time,and the immersion time was set as 60 seconds.
相似文献/References:
[1]王明洁,吴雨蹊,段亚东,等.蓝莓栽培土壤改良技术研究[J].北方园艺,2014,38(14):179.
WANG Ming-jie,WU Yu-xi,DUAN Ya-dong,et al.Improvement Research of Technique on Cultivation Soil of Blueberry[J].Northern Horticulture,2014,38(24):179.
[2]王贺春.蓝莓冬季修剪技术[J].北方园艺,2013,37(13):58.
WANG He-chun.Winter Pruning Techniqne of Blueberry[J].Northern Horticulture,2013,37(24):58.
[3]聂飞,张玉春.我国蓝莓产业发展的机遇与挑战[J].北方园艺,2014,38(04):165.
NIE Fei,ZHANG Yu-chun.Opportunities and Challenges for the Development of Blueberry Industry in China[J].Northern Horticulture,2014,38(24):165.
[4]杨芩,任永权,廖优江,等.五个兔眼蓝莓品种有效可授期研究[J].北方园艺,2013,37(14):5.
YANG Qin,REN Yong-quan,LIAO You-jiang,et al.Study on Effective Pollination Period of Five Cultivars of Rabbiteye Blueberry[J].Northern Horticulture,2013,37(24):5.
[5]傅 冰,洪 震,刘跃钧,等.蓝莓SRAP-PCR反应体系的建立和优化及其遗传多样性分析[J].北方园艺,2013,37(17):95.
FU Bing,HONG Zhen,LIU Yue-jun,et al.Optimization and Establishment of SRAP-PCR Reaction System of Vaccinium ssp. and Analysis of the Genetic Diversity[J].Northern Horticulture,2013,37(24):95.
[6]张文英,周 婧.低糖蓝莓番茄复合果酱的研制[J].北方园艺,2013,37(17):142.
Brewing Technology Engineering Research Center,Department of Food Science,Jilin Agricultural Science and Technology College,et al.Preparation of Low-sugar Jam with Blueberry and Tomato[J].Northern Horticulture,2013,37(24):142.
[7]龚 娜,肖 军,杨 镇,等.不同菌根真菌对蓝莓根际土壤微生物数量的影响[J].北方园艺,2013,37(17):175.
GONG Na,XIAO Jun,YANG Zhen,et al.Effects of Different Mycorrhizal Fungi on Soil Microbial Number in the Blueberry Rhizosphere[J].Northern Horticulture,2013,37(24):175.
[8]王贺春.适于丹东地区温室栽培的蓝莓引种与筛选[J].北方园艺,2013,37(12):33.
WANG He-chun.Introduction and Selection of Blueberry Varieties for Greenhouse That Suitable for Dandong Region[J].Northern Horticulture,2013,37(24):33.
[9]吴立仁,段亚东,杜汉军,等.黑龙江省蓝莓种植应该注意的几个问题[J].北方园艺,2013,37(05):50.
WU Li-ren,DUAN Ya-dong,DU Han-jun,et al.Several Problems Existing in Planting of Blueberry in Heilongjiang Province[J].Northern Horticulture,2013,37(24):50.
[10]刘 波.果胶酶对蓝莓果汁澄清效果的研究[J].北方园艺,2014,38(03):127.
LIU Bo.Study on Clarification Effect of Pectinase on Blueberry Juice[J].Northern Horticulture,2014,38(24):127.
备注/Memo
第一作者简介：宗树斌（1982-)，男，硕士，副教授，现主要从事植物工厂化繁育技术等研究工作。E-mail：zongsb_1982@163.com.基金项目：江苏省林业三新工程资助项目（LYSX[2016]46）；常州市农业三项更新工程资助项目。收稿日期：2018-06-10