[1]谢晋,滕长才,赵志刚,等.五十五份小白菜自交系的SSR遗传多样性分析[J].北方园艺,2018,42(12):7-12.[doi:10.11937/bfyy.20180548]
　XIE Jin,TENG Changcai,ZHAO Zhigang,et al.Analysis on Genetic Diversity of Fifty-five Brassica campestris L.by  SSR Markers[J].Northern Horticulture,2018,42(12):7-12.[doi:10.11937/bfyy.20180548]
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五十五份小白菜自交系的SSR遗传多样性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年12 页码: 7-12 栏目: 出版日期: 2018-05-01
Title:
Analysis on Genetic Diversity of Fifty-five Brassica campestris L.by  SSR Markers
作者:
谢晋; 滕长才; 赵志刚; 余青兰; 赵洪朝
（青海大学 农林科学院，春油菜研究所，国家油料改良青海分中心，农业部春油菜科学观测实验站，青海省春油菜遗传改良重点实验室，青海省春油菜研究开发中心,青海 西宁 810016）
Author(s):
XIE Jin; TENG Changcai; ZHAO Zhigang; YU Qinglan; ZHAO Hongchao
(Institute of Spring Rapeseed, Academy of Agriculture and Forestry Sciences, Qinghai University/The Qinghai Research Branch of The National Rapeseed Genetic Improvement Center/Scientific Observing and Experimental Station of Spring Rapeseed Ministry of Agriculture/Key Laboratory of Qinghai Province for Spring Rapeseed Genetic Improvement/Spring Rapeseed Research and Development Center of Qinghai Province, Xining, Qinghai 810016)
关键词:
小白菜; SSR; 遗传多样性; 种质资源
Keywords:
Brassica campestris L.; SSR; genetic diversity; germplasm resources
DOI:
10.11937/bfyy.20180548
文献标志码:
A
摘要:
以55份小白菜自交系为试材，采用SSR分子标记技术对其遗传多样性进行分析，以期为育种中杂交组合的选配及种质创新提供参考依据。结果表明：从分布在10条染色体上的110对引物中筛选出44对稳定的多态性引物，得到105个多态性位点，平均每对引物扩增的条带数为2.4，每条染色体上平均多态性位点为10.5个，遗传距离在0.016 9～0.754 4，平均为0.373 0，参试材料间差异较大；聚类分析将55 份材料分为四大类群，其中第Ⅰ大类31份材料，第Ⅱ大类8份材料，第Ⅲ大类5份材料，第Ⅳ大类11份材料。在这些材料中，大多数来源相同的聚为一类，少部分个体分布在不同类群中，可能是由于这些材料在人为与自然选择以及栽培环境的影响下发生了变异。
Abstract:
55 Brassica campestris L.inbred lines were used as experimental materials,the SSR molecular marker technique was used to study the genetic diversity of Brassica campestris L.in order to provide references for the selection of breeding combinations and germplasm innovation in breeding.The results showed that 44 pairs of stable polymorphic primers were screened out from 110 pairs of primers distributed on 10 chromosomes using SSR molecular markers,and 105 polymorphic loci were obtained.The number of bands amplified by each pair of primers was 2.4,and the average number of polymorphic sites per chromosome was 10.5.The genetic distance ranged from 0.016 9 to 0.754 4 with an average of 0.373 0.The differences among the tested materials were relatively large.55 clusters of materials were divided into 4 groups according to the cluster analysis,of which 31 were in the first category and 31 were in the second category.A total of 8 materials,Category III,Group 5,and Group IV,11 units.Among these materials,most of the same sources are clustered together,and a few individuals are distributed in different groups.This might be due to the variation of these materials under the influence of human and natural selection and cultivation environment.

参考文献/References:

 

[1]陈海梅.复混肥对油白菜生长的影响[J].北方园艺,2010(20):55-56.

[2]安风云,赵洪朝.青杂油白菜1号[J].中国种业,2008(2):74.

[3]周杰.白菜遗传多样性研究[D].武汉：华中农业大学,2009.

[4]郭晶心,曹鸣庆.芸薹属植物起源、演化及分类的分子标记研究进展[J].生物技术通报,2001(1):26-30.

[5]李桂花,陈汉才,张艳,等.小白菜种质遗传多样性与亲缘关系的SRAP 和SSR 分析[J].广东农业科学,2017,44(5):37-45.

[6]周乃元.芸薹类蔬菜遗传多样性的AFLP分子标记分析[D].北京:首都师范大学,2001.

[7]刘运霞.花椰菜种质资源遗传多样性的分析[D].北京:中国农业科学院,2012.

[8]王凯华,张文英,王会,等.国内外甘蓝型油菜种质SSR标记遗传多样性分析[J].中国农学通报,2011,27(19):144-149.

[9]PANG W X,LI X N,CHOI S R,et al.Development of a leafy Brassica rapa fixed line collection for genetic diversity and population structure analysis[J].Mol Breeding,2015(35):54.

[10]HASAN M,SEYIS F,BADANI A G,et al.Analysis of genetic diversity in the Brassica napus L.gene pool using SSR markers[J].Genetic Resources and Crop Evolution,2006,53:793-802.

[11]明佳熠.新型芥菜型油菜的创建及遗传多样性分析[D].重庆：西南大学,2016.

[12]赵会彦.芥菜型油菜多室基因Bjln1定位的研究[D].西宁：青海大学,2014.

[13]曾斌,田嘉,李疆.新疆核桃8个天然群体遗传多样性的SSR评价[J].新疆农业科学,2012,49(12):2180-2188.

[14]SHEN Z,ZHANG K Q,MA L Y,et al.Analysis of the genetic relationships and diversity among 11 populations of Xanthoceras sorbifolia using phenotypic and microsatellite marker data[J].Electronic Journal of Biotechnology,2017(26):33-39.

[15]罗冉,吴委林,张旸,等.SSR分子标记在作物遗传育种中的应用[J].基因组学与应用生物学,2010,29(1):137-143.

[16]BAE K M，SIM S C，HONG J H，et al.Development of genomic SSR genetic diversity analysis in cultivated radish (Raphanus sativus L.)[J].Hort Environ Biotechnol,2015,56(2):216-224.

[17]GAO J T,LI N,XUAN Z Y,et al.Genetic diversity among ‘Qamgur’ varieties in China revealed by SSR markers[J].Euphytica,2017,213:204.

[18]李丽,王海岗,张晓丽,等.SSR分子标记在作物遗传育种中的应用[J].山西农业科学,2008(3):15-18.

[19]田骏.种质资源遗传多样性研究进展[J].草业与畜牧,2012(10):53-58.

[20]代攀虹,孙君灵,何守朴,等.陆地棉核心种质表型性状遗传多样性分析及综合评价[J].中国农业科学,2016,49(19):3694-3708.

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备注/Memo

第一作者简介：谢晋（1994-），女，硕士研究生，研究方向为春油菜分子细胞遗传学。E-mail：18797375176@163.com.责任作者：赵洪朝（1973-），男，硕士，研究员，研究方向为十字花科蔬菜作物杂种优势研究与利用。E-mail:qaazhc@163.com.基金项目：国家重点研发计划资助项目（2016YFD0100202）；农业部948资助项目（2014-Z58）；青海省春油菜遗传改良重点实验室资助项目（2017-ZJ-Y09）；青海省春油菜研究开发中心能力建设资助项目（2015-GX-Q15A）；青海省科技厅推广资助项目（2015-NK-111）。收稿日期：2018-03-21

更新日期/Last Update: 2018-07-03