[1]杨珍福,郭相,汤昕明,等.一种红菇属野生菌的分离培养与鉴定[J].北方园艺,2018,42(15):175-179.[doi:10.11937/bfyy.20180333]
　YANG Zhenfu,GUO Xiang,TANG Xinming,et al.Isolation,Cultivation and Identification for One Wild Fungus of Genus Russula[J].Northern Horticulture,2018,42(15):175-179.[doi:10.11937/bfyy.20180333]
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一种红菇属野生菌的分离培养与鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年15 页码: 175-179 栏目: 出版日期: 2018-06-15
Title:
Isolation,Cultivation and Identification for One Wild Fungus of Genus Russula
作者:
杨珍福; 郭相; 汤昕明; 冯云利; 岳万松; 桂明英
（中华全国供销合作总社 昆明食用菌研究所，云南 昆明 650221）
Author(s):
YANG Zhenfu; GUO Xiang; TANG Xinming; FENG Yunli; YUE Wansong; GUI Mingying
(Institute of Kunming Edible Fungi,All China Federation of Supply and Marketing Cooporatives,Kunming,Yunnan 650221)
关键词:
绒紫红菇; 菌种分离; 培养; 鉴定
Keywords:
Russula mariae; isolation; cultivation; identification
DOI:
10.11937/bfyy.20180333
文献标志码:
A
摘要:
对采自云南楚雄的1种野生红菇属真菌的子实体进行组织分离，获得的菌丝体经纯化培养后进行液体培养，培养的菌丝体用于ITS鉴定。结果表明:改良的PDA培养基对红菇分离效果最佳，红菇菌丝白色、菌落较小，长势极慢；液体培养菌丝体生长缓慢，培养时间较长；菌丝球经过分子鉴定后确定为绒紫红菇（Russula mariae）。
Abstract:
The fruiting body of Russula collected from Chuxiong,Yunnan Province,was used to obtain pure culture.The mycelia was cultured in liquid medium,and the mycelia was used for ITS identification.The results showed that the improved PDA was the best medium for isolation from the fruiting body of Russula mariae,and the hyphae were white and small,and the growth rate was extremely slow.The mycelia grew slowly by liquid culture.Hyphae ball were determined as Russula mariae.

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备注/Memo

第一作者简介：杨珍福（1988-），女，硕士，助理研究员，研究方向为食用菌菌种分离培养。E-mail:ynjcyzf152@126.com.责任作者：桂明英（1965-），女，博士，研究员，研究方向为食用菌资源可持续利用。E-mail:guimingying@126.com.基金项目：国家科技支撑计划资助项目（2013BAD16B01）.收稿日期：2018-03-12

更新日期/Last Update: 2018-09-13