DU Fuqiang,HUANG Mingzhe,WEI Yibing,et al.Cutting Propagation of Miao Medicine Laportea bulbifera[J].Northern Horticulture,2018,42(18):126-131.[doi:10.11937/bfyy.20180276]
苗药红禾麻（Laportea bulbifera）扦插繁殖试验
- Title:
- Cutting Propagation of Miao Medicine Laportea bulbifera
- Keywords:
- Laportea bulbifera; cutting propagation; multiple comparison; principal component analysis
- 文献标志码:
- A
- 摘要:
- 为筛选出适于红禾麻扦插繁殖的外源性生长调节剂类型与处理方法，提高红禾麻扦插成活率。以红禾麻新鲜中部茎条为试材，研究了3种生长调节剂（IAA、IBA、NAA），4种浓度梯度（0(CK)、50、100、150、200 mg·L-1）与4个时长梯度（1、2、3、4 h）对其扦插繁殖的影响。处理后经45 d生长，观察记录其生根率、根数、根长、根系效果指数、叶片数、新茎数、新茎长等生长指标，并进行多重比较与主成分分析。结果表明：3种生长调节剂的不同浓度对红禾麻扦插繁殖的各指标均显著优于CK。但3种生长调节剂的不同浓度处理对红禾麻扦插繁殖的影响有一定差异。生根数随处理时间的增加而上升，但各处理间差异较小，生根数表现为NAA 200 mg·L-1 4 h>IAA 200 mg·L-1 4 h>IBA 200 mg·L-1 4 h；根系效果指数随3种生长调节剂浓度（50～200 mg·L-1）增加均呈显著上升趋势；主成分分析结果显示，NAA 200 mg·L-1 4 h综合得分最高，为1.583 8，排序第一，CK综合得分最低，为-2.915 5。因此，NAA 200 mg·L-1 4 h较利于红禾麻扦插繁殖。
- Abstract:
- To screen out the type and treatment methods of exogenous growth regulators suitable for the cutting propagation of Laportea bulbifera,further improved the survival percentage of Laportea bulbifera cutting.In this study,the fresh middle stem of Laportea bulbifera was used as the test material,and the effects of three growth regulators (IAA,IBA,NAA),four concentration gradients (0(CK),50,100,150,200 mg·L-1),and four time-length gradients(1,2,3,4 hours) on cutting propagation were studied.After 45 days of treatment,the growth indicators including rooting rate,root number,root length,root effect index,number of leaves,number of new stems,new stem length,etc. were observed and multiple comparisons and principal component analysis were performed.The results showed that the different concentrations of the three growth regulators were significantly better than CK for the cutting propagation of Laportea bulbifera.However,the effects of different concentrations of the three growth regulators on the cutting propagation of Laportea bulbifera were different.The number of rooting increased with the increase of processing time,but the results had little difference between the treatment groups.The effects of three growth regulators on the number of rooting in the groups were studied,and the NAA 200 mg·L-1 4 hours>IAA 200 mg·L-1 4 hours>IBA 200 mg·L-1 4 hours>IBA 200 mg·L-1 4 hours.The root effect indexes significantly increased with the increase of three growth regulators (50-200 mg·L-1) concentration.The principal component analysis results showed that the comprehensive score of NAA 200 mg·L-1 4 hours was the highest,it was 1.583 8,ranking at the first,and the control group comprehensive score was the lowest,the score was -2.915 5.Therefore,the treatment of NAA 200 mg·L-1 4 hours was more conductive to the cutting propagation of Laportea bulbifera.
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备注/Memo
第一作者简介：杜富强(1992-)，男，硕士研究生，研究方向为药用植物资源应用与开发。E-mail:1245700319@qq.com.责任作者：魏升华（1965-），男，本科，教授，现主要从事中药民族药资源及鉴定与栽培技术等研究工作。E-mail:weishenghua6512@126.com.基金项目：国家中医药管理局资助项目（2013—2015）；贵州省教育厅“十二五”重大科技专项资助项目（黔教合重大专项字[2012]010号）；贵州省科技计划资助项目（黔科合LH字[2016]7113号）；贵阳中医学院大学生创新创业训练计划资助项目(贵中医大创合字[2016]16号)；药用植物功效利用国家重点实验室开放课题资助项目（FAMP201701K）；贵州省教育厅资助项目（黔教合KY字[2017]018）。收稿日期：2018-03-19