[1]刘海双,秦红艳,石广丽,等.基于叶绿体基因的猕猴桃属植物系统发育分析[J].北方园艺,2018,42(17):26-31.[doi:10.11937/bfyy.20174730]
　LIU Haishuang,QIN Hongyan,SHI Guangli,et al.Phylogenetic Analysis of Actinidia Based on Chloroplast Genes[J].Northern Horticulture,2018,42(17):26-31.[doi:10.11937/bfyy.20174730]
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基于叶绿体基因的猕猴桃属植物系统发育分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年17 页码: 26-31 栏目: 出版日期: 2018-09-10
Title:
Phylogenetic Analysis of Actinidia Based on Chloroplast Genes
作者:
刘海双; 秦红艳; 石广丽; 孙丹; 艾军; 李昌禹
（中国农业科学院 特产研究所，吉林 长春 130000）
Author(s):
LIU Haishuang; QIN Hongyan; SHI Guangli; SUN Dan; AI Jun; LI Changyu
(Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun,Jilin 130000)
关键词:
matK; rbcL; 猕猴桃属; 系统发育
Keywords:
matK; rbcL; Actinidia; phylogenetic
DOI:
10.11937/bfyy.20174730
文献标志码:
A
摘要:
以7份猕猴桃资源为试材，结合GenBank数据库中已有的猕猴桃属植物相关序列，采用叶绿体基因标记matK和rbcL构建K2P遗传距离矩阵及NJ系统发育树，研究了猕猴桃属植物种间亲缘关系，以期为猕猴桃属植物属下分类研究提供参考。结果表明：叶绿体序列系统发育分析结果与现行的“四组四系”分类系统不符，净果组与其余3组K2P遗传距离较远，组内实髓系与片髓系之间遗传差距较大，支持净果组分为实髓系与片髓系两系的分类方法；星毛组、糙毛组及斑果组物种相互混杂，难以划分，建议3组合并为“非净果组”，原有三组分别降级为“星毛系、糙毛系和斑果系”，建立“二组五系”的猕猴桃属植物属下分类系统。
Abstract:
Seven kiwifruit resources were used as materials,and relating Actinidia plant sequences were downloaded from GenBank database.K2P genetic distance matrix and NJ phylogenetic tree were constructed by using cpDNA marker matK and rbcL and the interspecies relationship of Actinidia was analyzed in order to provide some useful information for the study on the classification of Actinidia.The results suggested that the current ‘four groups and four lines’ classification system did not consent with our conclusion.The genetic distance between Sect.Leiocarpae Dunn and other three groups and 2 lines of Sect.Leiocarpae Dunn were long.So it was suggested that Sect.Leiocarpae Dunn conformed to two lines.The other three groups mixed each other,it was difficult to be divided,so this study suggested a combination of those three groups which downgraded to three lines.In conclusion it was suggested a ‘two groups and five lines’ Actinidia classification system.

相似文献/References:

[1]刘青青,杨开宇,马玉花,等.沙棘rbcL基因的扩增及序列分析[J].北方园艺,2023,(21):42.[doi:10.11937/bfyy.20231588]
　LIU Qingqing,YANG Kaiyu,MA Yuhua,et al.Amplification and Sequence Analysis of rbcL Gene in Hippophae rhamnoides[J].Northern Horticulture,2023,(17):42.[doi:10.11937/bfyy.20231588]

备注/Memo

第一作者简介：刘海双（1993-），男，硕士研究生，研究方向为浆果资源的分子标记。E-mail：liuhaishuang1993@163.com.责任作者：李昌禹（1971-），男，硕士，研究员,现主要从事特种作物育种与分子生物学等研究工作。E-mail：tcslicy@163.com.基金项目：中央级公益性科研院所基本科研业务费专项资助项目（1610342016019）；吉林省科技发展计划资助项目（20170203006NY）。收稿日期：2018-03-23

更新日期/Last Update: 2018-09-30