DIAO Yi,ZHOU Lijuan.ISSR Analysis on Genetic Diversity of Nostoc commune[J].Northern Horticulture,2018,42(14):143-147.[doi:10.11937/bfyy.20174695]
地木耳遗传多样性的ISSR分析
- Title:
- ISSR Analysis on Genetic Diversity of Nostoc commune
- Keywords:
- Nostoc commune; ISSR; genetic diversity
- 文献标志码:
- A
- 摘要:
- 采用ISSR分子标记技术对7个居群的地木耳进行了遗传多样性研究。结果表明：8条ISSR引物共检测到41条清晰的谱带，其中，多态性条带40条；地木耳在物种水平上遗传多样性较高，其PPB为97.56%、He为0.411 4、I为0.598 1；在居群水平上遗传多样性相对较低，其PPB为43.90%、He为0.165 4、I为0.246 3；居群间遗传变异较高，其中，Gst为0.597 3，PHIst为0.613 4，(Hsp-Hpop)/Hsp为0.588 2；聚类分析发现，以遗传一致度0.72为标准，7个地木耳居群可分为4类。地木耳物种水平遗传多样性大于居群水平，居群间遗传变异大于居群内。
- Abstract:
- In this study,genetic diversity among seven populations of Nostoc commune were analyzed by using ISSR molecular techniques.The results showed that there were 40 polymorphic loci among 41 ISSR loci by 8 ISSR primers;there were higher level of genetic diversity at species level (PPB =97.56%,He=0.4114,I=0.598 1) than at population level(PPB=43.90%,He=0.165 4,I=0.246 3);a high level of genetic differentiation existed in populations:Gst=0.597 3,PHIst =0.613 4,(Hsp-Hpop)/Hsp=0.588 2;and the 7 populations were divided into 4 groups by cluster analysis.There was a higher level of genetic diversity at species level than at populations level,a higher level of genetic differentiation existed among populations than within populations.
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备注/Memo
第一作者简介：刁毅（1975-），男，四川南部人，博士，教授，现主要从事资源微生物与植物抗病性等研究工作。E-mail:diaoy163@163.com.基金项目：干热河谷特色生物资源开发四川省高等学校重点实验室资助项目（GR-2013-E-01）；攀枝花学院博士基金资助项目（BKQJ2015030）；攀西特色资源大学生创新实验室（苗子工程项目）资助项目（2015RZ0029）。收稿日期：2018-03-07