[1]张永福,李利红,莫丽玲,等.茶多酚和咖啡因对缓解葡萄铝毒的生理机制[J].北方园艺,2018,42(08):1-6.[doi:10.11937/bfyy.20174536]
　ZHANG Yongfu,LI Lihong,MO Liling,et al.Physiological Mechanism of Tea Polyphenols and Caffeine on Relieving Aluminum Toxicity in Grape[J].Northern Horticulture,2018,42(08):1-6.[doi:10.11937/bfyy.20174536]
点击复制

茶多酚和咖啡因对缓解葡萄铝毒的生理机制

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年08 页码: 1-6 栏目: 出版日期: 2018-04-30
Title:
Physiological Mechanism of Tea Polyphenols and Caffeine on Relieving Aluminum Toxicity in Grape
作者:
张永福1; 李利红1; 莫丽玲1; 牛燕芬1; 王定斌2
1.昆明学院 农学院，云南 昆明 650214；2.宣威市阿都乡农业综合服务中心，云南 宣威 655425
Author(s):
ZHANG Yongfu1;  LI Lihong1;  MO Liling1;  NIU Yanfen1;  WANG Dingbin2
1.School of Agriculture, Kunming University, Kunming,Yunnan 650214； 2. Comprehensive Agricultural Service Center of Adu Township of Xuanwei City, Xuanwei, Yunnan 655425
关键词:
茶多酚; 咖啡因; 葡萄; 铝毒; 生理机制
Keywords:
tea polyphenol;  caffeine;  grape;  aluminum toxicity;  physiological mechanism
DOI:
10.11937/bfyy.20174536
文献标志码:
A
摘要:
以葡萄苗为试材，采用无土栽培的方法，研究了10 mmol·L-1硫酸铝钾胁迫下，0.25、0.50、0.75、1.00 mg·L-1的茶多酚和咖啡因处理对缓解葡萄铝毒的生理效应，以期为葡萄的耐铝栽培提供参考依据。结果表明：铝胁迫下葡萄生物量、叶绿素含量、根系活力下降，根冠比上升；叶片蛋白质、脯氨酸和过氧化氢的含量，及POD、SOD的活性上升；叶片和根系的丙二醛含量和氧自由基产生速率也上升。但在加入茶多酚和咖啡因后，葡萄生物量、叶绿素含量、根系活力、叶片POD和SOD活性，以及叶片和根系蛋白质和脯氨酸的含量均高于铝胁迫处理；而根冠比、叶片和根系的氧自由基产生速率、过氧化氢和丙二醛的含量则低于铝胁迫处理。茶多酚和咖啡因处理能够降低铝胁迫对葡萄植株生长的抑制作用，缓解铝毒对葡萄的伤害，效果最显著的是0.75 mg·L-1茶多酚和0.50 mg·L-1咖啡因处理。
Abstract:
The grape seedlings were used as materials, physiological effects of tea polyphenols and caffeine treatment of 0. 25 mg?L-1, 0. 50 mg?L-1, 0. 75 mg?L-1 and 1. 00mg?L-1, on relieving grape aluminum toxicity were studied by soilless cultivation under 10 mmol?L-1 aluminum sulfate stress, in order to provide reference basis for aluminum tolerance cultivation of grape. The results showed that under the aluminum stress, the biomass, chlorophyll content, root activity and root shoot ratio of grape increased; the contents of protein, proline and hydrogen peroxide in leaves, and the activities of POD and SOD increased; the leaves and roots of malondialdehyde content and oxygen free radical production rate also rose. But after adding tea polyphenols and caffeine, grape biomass, chlorophyll content, root activity, POD and SOD activity in leaves, and the content of protein and proline in leaves and roots were higher than that of aluminum stress; and the root shoot ratio, the oxygen free radical generation rate, the contents of hydrogen peroxide and malondialdehyde in leaves and roots were lower than that of aluminum stress. Tea polyphenols and caffeine treatment can reduce the inhibitory effect on the growth of grape plant aluminum stress, alleviate Al toxicity on grape damage. Among them, the most significant effect is 0.75 mg?L-1 polyphenols and 0.50 mg?L-1 caffeine treatment.

参考文献/References:

[1]ARENHART R A,de LIMA J C,PEDRON M,et al.Involvement of ASR genes in aluminium tolerance mechanisms in rice[J].Plant Cell and Environment,2013,36:52-67.

[2]NAGATA T,HAYATSU M,KOSUGE N.Identification of aluminium forms in tea leaves by 27Al-NMR [J].Phytochemistry,2002,31(4):1215-1218.
[3]LIU S M,OU S Y,HUANG H H.Green tea polyphenols induce cell death in breast cancer MCF-7 cells through induction of cell cycle arrest and mitochondrial-mediated apoptosis[J].Zhejiang Univ-Sci B (Biomed & Biotechnol),2017,18(2):89-98.
[4]刘寒旸,宋军,周艳,等.咖啡因通过Caspase通路促进胃癌细胞凋亡的研究[J]. 实用临床医药杂志, 2017, 21(13): 40-44.
[5]于翠平.茶树耐铝基因型差异及机理研究[D].杭州：浙江大学, 2014.
[6]张永福,任禛,陈泽斌,等.水杨酸对缓解葡萄苗铝毒害的生理机制[J].华北农学报,2015,30(1):182-187.
[7]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.
[8]张志良,瞿伟菁,李小方.植物生理学实验指导(4版)[M].北京:高等教育出版社,2009.
[9]LANGER H,CEA M,CURAQUEO G,et al.Influence of aluminum on the growth and organic acid exudation in alfalfa cultivars grown in nutrient solution[J].Journal of Plant Nutrition,2009,32(4):618-628.
[10]DURESSA D,SOLIMAN K M,TAYLOR R W,et al.Gene expression profiling in soybean under aluminum stress:Genes differentially expressed between Al-tolerant and Al-sensitive genotypes[J].American Journal of Molecular Biology,2011,(1):156-173.
[11]张永福,黄鹤平,彭声静,等.铝胁迫下水杨酸对水晶葡萄植株生长及营养积累的影响[J].中外葡萄与葡萄酒,2015,(2):10-15.
[12]郭有燕,刘宏军,孔东升,等.干旱胁迫对黑果枸杞幼苗光合特性的影响[J].西北植物学报,2016,36(1):124-130.
[13]DUAN J J,GUO S R.Effects of exogenous spermidine on tolerance of cucumber seedlings under NaCl stress[J].China Vegetables,2005,(12):8-10.
[14]CHEN L S,QI Y P,SMITH B R,et al.Aluminum-induced decrease in CO2 assimilation in citrus seedlings is unaccompanied by decreased activities of key enzymes involved in CO2 assimilation[J].Tree Physiology,2005,25(3):317-324.
[15]JIANG H X,TANG N,ZHENG J G,et al.Phosphorus alleviates aluminum-induced inhibition of growth and photosynthesis in Citrus grandis seedlings[J].Physiologia Plantarum,2009,137:298-311.
[16]JIANG H X,TANG N,ZHENG J G,et al.Antagonistic actions of boron against inhibitory effects of aluminum toxicity on growth, CO2 assimilation, ribulose-1,5-bisphosphate carboxylase /oxygenase, and photosynthetic electron transport probed by the JIP-test, of Citrus grandis seedlings[J].BMC Plant Biology,2009(9):102.
[17]YANG L T, QI Y P, CHEN L S,et al.Nitric oxide protects sour pummelo (Citrus grandis) seedlings against aluminum-induced inhibition of growth and photosynthesis[J].Environmental and Experimental Botany,2012,83:1-13.
[18]MAHAJAN S, TUTEJA N.Cold,salinity and drought stress:An overview[J].Archives of Biochemistry and Biophysics,2005,444(2):139-158.
[19]孔繁翔,桑伟莲,蒋新,等.铝对植物毒害及植物抗铝作用机理[J].生态学报,2000,20(5):855-862.
[20]ASHRAF M, FOOLAD M R.Roles of glycine betaine and proline in improving plant abiotic stress resistance[J].Environmental and Experimental Botany,2007,59(2):206-216.
[21]李交昆,唐璐璐.植物抗铝分子机制研究进展[J].生命科学,2013,(6):588-594.
[22]YAMAMOTO Y, KOBAYASHI Y, DEVI S R,et al.Aluminum toxicity is associated with mitochondrial dysfunction and the production of reactive oxygen species in plant cells[J].Plant Physiogy,2002,128:63272.
[23]于力,孙锦,郭世荣,等.铝胁迫对豇豆幼苗根尖抗氧化系统的影响[J].细胞植物学报,2012, 32(1):2299-2304.
[24]潘昕,邱权,李吉跃,等.干旱胁迫对青藏高原6种植物生理指标的影响[J].生态学报,2014, 34(13):3558-3567.
[25]张盛楠,刘亚敏,刘玉民,等.马尾松幼苗生长及生理特性对铝胁迫的响应[J].西北植物学报, 2016,36(10):2022-2029.

相似文献/References:

[1]曲芬霞.茶多酚对马蹄的常温保鲜效果[J].北方园艺,2012,36(05):162.
　QU Fen-xia.Effects of Tea Polyphenols on Water Chstnut of Room Temperature Preservation[J].Northern Horticulture,2012,36(08):162.
[2]闫列娟,肖斌,索罗丹,等.陕南黑毛茶加工工艺研究[J].北方园艺,2012,36(10):172.
　YAN Lie-juan,XIAO Bin,SUO Luo-dan,et al.Dark Green Tea Processing Craft in Southen Shaanxi[J].Northern Horticulture,2012,36(08):172.
[3]赵 莉.茶多酚与海藻酸钠膜对鲜切雏菊保鲜的影响[J].北方园艺,2015,39(11):124.[doi:10.11937/bfyy.201511031]
　ZHAO Li.Effect of Tea Polyphenol Incorporated into Sodium Alginate-based Edible Coating on Preservation of Fresh-cut Bellis perennis[J].Northern Horticulture,2015,39(08):124.[doi:10.11937/bfyy.201511031]
[4]李 翠 英１,２,叶 新 华 ２,等.茶多酚处理后杏果实贮藏期超氧阴离子产生及 Ｍ Ｄ Ａ 含量的变化[J].北方园艺,2011,35(24):0.[doi:10.11937/bfyy.2011024063]
　,l,et al.ＣｈａｎｇｅｓｏｆＳｕｐｅｒｏｘｉｄｅＡｎｉｏｎＲａｄｉｃａｌＧｅｎｅｒａｔｉｏｎａｎｄ Ｍａｌｏｎａ ｌ ｄ ｅ ｈ ｙ ｄ ｅ 　Ｃ ｏ ｎ ｔ ｅ ｎ ｔｉ ｎ 　Ａ ｐ ｒ ｉ ｃ ｏ ｔ 　Ｆ ｒ ｕ ｉ ｔ ｓ 　Ａ ｆ ｔ ｅ ｒ 　Ｔ ｅ ａ 　Ｐ ｏ ｌ ｙ ｐ ｈ ｅ ｎ ｏ ｌ 　Ｔ ｒ ｅ ａ ｔ ｍ ｅ ｎ ｔ 　Ｄ ｕ ｒ ｉ ｎ ｇ Ｓ ｔ ｏ ｒ ａ ｇ ｅ[J].Northern Horticulture,2011,35(08):0.[doi:10.11937/bfyy.2011024063]
[5]朱旋,田芸,孙海燕,等.茶多酚结合超高压处理对鲜切天麻贮藏品质的影响[J].北方园艺,2024,(20):83.[doi:10.11937/bfyy.20241299]
　ZHU Xuan,TIAN Yun,SUN Haiyan,et al.Effects of Tea Polyphenols Combined With High Hydrostatic Pressure on Storage Quality of Fresh-cut Gastrodia elata Bl.[J].Northern Horticulture,2024,(08):83.[doi:10.11937/bfyy.20241299]

备注/Memo

第一作者简介：张永福（1981-），男，云南弥勒人，博士，教授，现主要从事果树抗性生理等研究工作。E-mail: 123017360@qq.com.基金项目：国家自然科学基金资助项目（31660559）；云南省应用基础研究计划资助项目（2017FD087）；昆明学院科学研究资助项目（XJZZ1604）。收稿日期：2018-02-05

更新日期/Last Update: 2018-02-28