ZHANG Ruochen,CHEN Dongli,WANG Liangmin.Genetic Diversity of Acer mono Maxim.in Shanxi Based on SCoT Markers[J].Northern Horticulture,2018,42(12):95-100.[doi:10.11937/bfyy.20174365]
基于SCoT标记的山西五角枫遗传多样性分析
- Title:
- Genetic Diversity of Acer mono Maxim.in Shanxi Based on SCoT Markers
- Keywords:
- SCoT; molecular marker; Acer mono Maxim.; genetic diversity
- 文献标志码:
- A
- 摘要:
- 以同一生境的40份自然分布的五角枫为试材，采用SCoT分子标记技术，对霍山兴唐寺的五角枫群体进行遗传多样性分析，为五角枫种质资源的评价和保护提供分子生物学依据。结果表明：筛选的15条SCoT共扩增出113条条带，其中多态性条带41条，多态性比例为36.28%；等位基因数平均为1.362，有效等位基因数平均为1.23，Nei′s基因多样性平均为0.134 1，香农信息指数平均为0.199 4；平均相似系数为0.945。试验结果说明供试材料种群的遗传多样性不高，遗传基础较为狭窄。五角枫的遗传多样性评价应该结合形态学表型与更多的分子标记技术。
- Abstract:
- To estimate genetic diversity of Acer mono maxim.in Huoshan Mount of Shanxi Province，40 germplasm resources were studied by SCoT molecular markers.The results showed that 113 bands were amplified by 15 SCoT primers，including 41 polymorphic bands,and the polymorphism rate was 36.28%.The average of observed number of alleles,effective number of alleles,Nei′s gene diversity,Shannon information index,and similarity coefficient were 1.362,1.23,0.134 1,0.199 4 and 0.945.It indicated that the genetic diversity occurred within Acer mono Maxim.was poor，and the genetic basis was narrow.The estimate of the genetic diversity of Acer mono Maxim.in Huoshan Mount of Shanxi Province should be combined with morphological phenotype and more molecular marker techniques.
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备注/Memo
第一作者简介：张若晨（1986-），女，硕士，讲师，研究方向为园林树木栽培。E-mail：zhangruochen2009@126.com.基金项目：国家自然科学基金资助项目（31400333）。收稿日期：2018-02-01