SUN Hongyan,ZHOU Zhaowei,WANG Xiaoyun,et al.Regulatory Effects of Different Exogenous Growth Regulators on Cadmium Toxicity for Cucumber[J].Northern Horticulture,2018,42(09):68-72.[doi:10.11937/bfyy.20174183]
不同外源生长调节剂对黄瓜镉毒害的调控作用
- Title:
- Regulatory Effects of Different Exogenous Growth Regulators on Cadmium Toxicity for Cucumber
- 文献标志码:
- A
- 摘要:
- 以黄瓜“津研4号”为试材，采用土培试验方法，研究了外源赤霉素（GA）、柠檬酸（HCA）、褪黑素（MLT）、亚硒酸钠（Se）单施或配施对70 mg?kg-1 Cd处理下黄瓜幼苗根长、株高、叶绿素含量及可溶性蛋白质含量的影响及其调控机制，以期为黄瓜的耐镉栽培提供参考依据。结果表明：镉处理会显著降低黄瓜根长、株高、叶绿素和可溶性蛋白质含量，而各类外源生长调节剂均对黄瓜镉毒害症状有所调控。外源喷施Se、GA和HCA+GA+MLT对Cd胁迫下黄瓜根长和株高生长有显著促进作用，使黄瓜幼苗根长生长基本恢复到对照水平；外源Se会显著升高黄瓜叶片叶绿素a含量、b含量、a+b总量和叶绿素a/b值，其中叶绿素b含量上升幅度最大，较单独镉处理升高52.3%；外源Se、HCA、Se+GA+MLT处理可使Cd胁迫下黄瓜可溶性蛋白质含量得到显著恢复。表明此4种外源生长调节剂在一定程度上均能调控黄瓜镉毒害，其中Se的调控作用最为明显，它对黄瓜镉毒害具有明显的缓解作用。
- Abstract:
- The cucumber variety ‘Jinyan 4’ was used as research material，the regulatory effects of different exogenous growth regulators under 70 mg?kg-1 Cd stress on root length，plant height，chlorophyll content and soluble protein content of cucumber seedlings were studied based on pot experiment，in order to provide reference basis for Cd tolerance cultivation of cucumber.The results showed that Cd treatment could significantly decrease cucumber root length，plant height，contents of chlorophyll and soluble protein，while exogenous growth regulators could regulate cucumber cadmium toxicity symptoms in different degree.Exogenous Se，GA and HCA+GA+MLT significantly promoted root length and height growth of cucumber under Cd stress，and the root length growth of cucumber seedlings returned to the control level.Exogenous Se significantly increased chlorophyll a content，chlorophyll b content，chlorophyll a+b content and chlorophyll a/b value in cucumber leaves，with the largest increase value in chlorophyll b content，which increased 52.3% compared with Cd treatment alone.Exogenous Se，HCA and Se+GA+MLT treatments could significantly restore soluble protein content in cucumber under Cd stress.It suggested that the four exogenous growth regulators could regulate cucumber cadmium toxicity to some extent，and the regulation of Se was the most obvious，which had distinct alleviation effect on cucumber cadmium toxicity.
参考文献/References:
[1]于肇端，王丽娜，曹辰兴，等.外源一氧化氮对镉胁迫下黄瓜幼苗生长、活性氧代谢和光合特性的影响[J].云南植物研究，2009，31(6):486-492. [2]蒋汉明，李书启，韩希凤，等.镉对植物生长的影响及植物耐镉机理研究进展[J].广东微量元素科学，2012，19(5):1-6. [3]冉烈，李会合.土壤镉污染现状及危害研究进展[J].重庆文理学院学报(自然科学版)，2011，30(4):69-73． [4]张晓敏，张秀英，钟太洋，等.中国农田土壤富集状况及其空间分布研究[J].环境科学，2014，35(2):692-703． [5]孟华兵.镉对油菜的毒害效应以及施用外源激素对镉毒害的调控作用[D].杭州:浙江大学，2007. [6]TASSI E，POUGET J E L，PETRUZZELLI G,et al.The effects of exogenous plant growth regulators in the phytoextraction of heavy metals[J].Chemosphere，2008，71(1):66-73. [7]何冰，陆覃昱，李彦彦，等.不同生长调节剂对东南景天镉积累的影响[J].农业环境科学学报，2014，33(8):1538-1545. [8]张玉芬，刘景辉，杨彦明，等.柠檬酸和EDTA对蓖麻生理特性和镉累积的影响[J].生态与农村环境学报，2015，31(5):760-766. [9]樊扬帆.外源螯合剂柠檬酸和NTA对苎麻修复重金属 Cd 污染土壤的研究[D].长沙:湖南大学，2015. [10]BELIGNI M V，LAMATTINA L.Is nitric oxide toxic or protective[J].Trends Plant Sci，1999，4(8):299-230. [11]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社，2000:165-167. [12]孙红艳，王杰文，王宇娜，等.硒对镉胁迫下黄瓜种子萌发及生长的影响[J].江苏农业科学，2016，44(12):175-177. [13]刘俊.镧、硒对镉胁迫下番茄、黄瓜产量品质及Cd含量的调控作用[D].重庆:西南大学，2014. [14]刘仕翔，黄益宗，罗泽娇，等.外源褪黑素处理对镉胁迫下水稻种子萌发的影响[J].农业环境科学学报，2016，35(6):1034-1041. [15]闵海丽,蔡三娟,徐勤松,等.外源钙对黑藻抗镉胁迫能力的影响[J].生态学报,2012,32(1):256-264. [16]吴敏，何鹏，韦家少，等.钾水平对巴西橡胶树幼苗叶片叶绿素及可溶性蛋白含量的影响[J].中国农学通报，2011，27(28):58-62. [17]张明生,谢波,谈锋,等.甘薯可溶性蛋白、叶绿素及ATP含量变化与品种抗旱性关系的研究[J].中国农业科学，2003，36(1):13-16.
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备注/Memo
第一作者简介：孙红艳（1983-），女，博士，副教授，现主要从事植物逆境生理与分子生物学等研究工作。E-mail：sunhongyan-8@163.com.基金项目：国家自然科学基金资助项目（31401319）；山西省青年科技研究基金资助项目（2015021146）；山西省高等学校优秀青年学术带头人支持计划资助项目（20151006）；山西省回国留学人员科研资助项目（2017-083）；太原科技大学博士启动基金资助项目（HY2013002）。收稿日期：2018-02-23