LIUJianxiang,WANGRuiling,LIYujiao,et al.ExtractionConditionofTotalFlavonoidFromBlueberryLeaves[J].Northern Horticulture,2018,42(20):125-130.[doi:10.11937/bfyy.20173987]
蓝莓叶黄酮提取条件的研究
- Title:
- ExtractionConditionofTotalFlavonoidFromBlueberryLeaves
- Keywords:
- blueberryleaves; flavonoids; ultrasound; orthogonaltest
- 文献标志码:
- A
- 摘要:
- 以废弃物蓝莓叶为原料，采用回流和超声2种方法，在单因素试验的基础上，采用正交实验优化了工艺条件，分别研究了溶剂浓度、提取温度、料液比、提取时间对蓝莓叶中的总黄酮得率的影响，确定了回流提取和超声提取的最佳条件，对提取产物总黄酮进行了显色稳定性试验和仪器精密度试验。结果表明：回流提取法的优化条件为料液比1∶5g·mL-1，提取温度100℃，提取时间90min，乙醇浓度70%，得率为7.24%；超声提取法的最佳提取条件为提取时间45min，温度为60℃，乙醇浓度70%，料液比1∶5g·mL-1，得率为7.18%。综合考虑，超声提取和回流提取优化后得率比较接近，二者相较而言，超声提取时间较短，对温度要求不高，可选择超声进行提取。
- Abstract:
- Takingblueberryleavesastherawmaterial,totalflavonoidsfromblueberryleaveswasextractedbyultrasonicmethodandrefluxextraction,theeffectsofextractionconditionssuchassolventconcentration,extractiontemperature,ratioofliquid-solid,extractiontimeofextractionwereinvestigatedbyone-factortestsandorthogonaltest.Thecolorstabilitytestandinstrumentprecisiontestoftotalflavonoidswerecarriedout.Theresultsshowedthatoptimumextractionconditionsoftotalflavonoidsfromblueberryleavesbyrefluxmethodweresolid-liquidratio1∶5g·mL-1,extractiontime90minutes,70%ethanolconcentration，temperature100℃，theaverageextractionrateoftotalflavonoidswas7.24%;theoptimumextractionconditionsbyultrasonicmethodwereextractiontime45minutes，temperature60℃,70%ethanolconcentration,solid-liquidratio1∶5g·mL-1,theaverageextractionrateoftotalflavonoidswas7.18%.Consideredthatultrasonicextractionandrefluxextractionwererelativelyclosetoeachother,theultrasonicextractiontimewasshortandthetemperaturerequirementwasnothigh.Ultrasonicextractioncouldbeselected.
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备注/Memo
第一作者简介：刘建祥（1976-），男，博士研究生，讲师，研究方向为天然产物的研究与利用。E-mail：liujx_swfc@126.com.责任作者：刘祥义（1964-），男，博士，教授，博士生导师，研究方向为生物资源的研究与开发。E-mail：liuxy11@126.com.基金项目：云南省优势特色重点学科生物学一级学科建设资助项目（50097505）；云南省高校科技创新团队资助项目（51400605）；云南省大学生创新创业计划资助项目（20170019）。收稿日期：2018-02-28