[1]石玉波,钱长根,卓丽环,等.百子莲花芽分化过程中DNA甲基化的差异分析[J].北方园艺,2018,42(16):99-104.[doi:10.11937/bfyy.20173594]
　SHI Yubo,QIAN Changgen,ZHUO Lihuan,et al.Analysis of DNA Methylation Differences in the Differentiation Process of Agapanthus praecox ssp.orientalis[J].Northern Horticulture,2018,42(16):99-104.[doi:10.11937/bfyy.20173594]
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百子莲花芽分化过程中DNA甲基化的差异分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年16 页码: 99-104 栏目: 出版日期: 2018-08-25
Title:
Analysis of DNA Methylation Differences in the Differentiation Process of Agapanthus praecox ssp.orientalis
作者:
石玉波1; 钱长根1; 卓丽环2; 王玲2
（1.嘉兴职业技术学院，浙江 嘉兴 314036；2.东北林业大学 园林学院，黑龙江 哈尔滨 150040）
Author(s):
SHI Yubo1; QIAN Changgen1; ZHUO Lihuan2; WANG Ling2
(1.Jiaxing Vocational and Techinical College,Jiaxing，Zhejiang 314036;2.College of Landscape and Architecture，Northeast Forestry University,Harbin，Heilongjiang 150040)
关键词:
百子莲; 花芽分化; DNA甲基化
Keywords:
Agapanthus praecox ssp.orientalis; flower bud differentiation; DNA methylation
DOI:
10.11937/bfyy.20173594
文献标志码:
A
摘要:
以4年生盆栽百子莲为试材，利用MSAP技术，对百子莲营养芽、诱导芽、花序芽进行甲基化水平和模式分析，以探讨其开花的表现性状与其基因组甲基化修饰的关系。结果表明：从64对引物中选出36对引物，分别获得292、310、323个电泳条带，其中无甲基化或单链内甲基化分别是184、204、210 条，双链内甲基化条带分别是93、108、107条，超甲基化条带数分别为 76、63、58条。3个样品中总体甲基化比例分别为61.34%、60.31%和 60.60%，该研究可为百子莲植株的分子鉴定、良种选育与栽培，以及遗传演化等提供新思路。
Abstract:
The levels and pattern of DNA methylation in 4-year-old potted Agapanthus vegetative bud,inductive bud,inflorescence bud were analyzed by methylation-sensitive amplification polymorphism marker (MSAP),in order to explore the correlation between flowering performance traits and their genome methylation modification.The results showed that 36 pairs were selected from 64 pairs of the MSAP primers,and 292,310,323 electrophoresis bands were obtained,respectively，no methylation or single chain methylation were 184,204,210 bands,respectively；the double chain methylation bands were 93,108,107 bands,respectively.The number of hypermethylated bands were 76,63,58 bands,respectively.The overall methylation ratios of the three samples were 61.34%,60.31%,60.60%.It provided new ideas for the molecular identification,seed breeding and cultivation,and genetic evolution of the plant by this study.

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备注/Memo

第一作者简介：石玉波（1982-），女，博士，讲师，研究方向为园林植物种质资源。E-mail:shiyubo2000@163.com.责任作者：王玲（1972-），女，博士，教授，博士生导师，研究方向为园林植物种质资源开发及园林植物应用。E-mail:wanglinghlj@126.com.基金项目：嘉兴市科技计划资助项目（2016AY23030）；2016年浙江省大学生科技创新活动计划暨新苗人才计划资助项目（2016R471003）；浙江省教育厅一般科研资助项目（Y201534301）。收稿日期：2018-02-02

更新日期/Last Update: 2018-09-30