ZENG Xiangda,LI Yankun,ZHUO Yinan,et al.Preliminary Study on Germination Inhibitors From Ilex asprella Fruits[J].Northern Horticulture,2018,42(07):133-138.[doi:10.11937/bfyy.20173046]
岗梅发芽抑制物质初步研究
- Title:
- Preliminary Study on Germination Inhibitors From Ilex asprella Fruits
- Keywords:
- Ilex asprella; germination inhibitor; systematic solvent extraction; germination rate; root length
- 文献标志码:
- A
- 摘要:
- 以白菜种子为试材，以新鲜岗梅果实不同部位（种子、果肉、果皮）为研究对象，采用系统溶剂提取的方法，提取不同极性提取物后稀释成不同浓度（0.100、0.067、0.033、0.017 g?mL-1）处理白菜种子并进行发芽试验，研究了不同提取物对白菜种子发芽及发芽后胚根长的影响，以期确定岗梅果实发芽抑制物存在的类型和存在部位。结果表明：岗梅果实3个部位中均含有内源性发芽抑制物。种子部位石油醚浸提物、水浸提物能显著抑制白菜种子的萌发；果肉部位石油醚浸提物、甲醇浸提物显著降低了白菜种子的发芽率，而乙酸乙酯浸提物则显著降低了白菜种子的胚根长；果皮部位乙酸乙酯、甲醇、水浸提物能显著抑制白菜种子的萌发，而石油醚浸提物、甲醇浸提物、水浸提物显著降低了白菜种子的胚根长。
- Abstract:
- Extracts of different parts (seed,flesh and peel) of fresh Ilex asprella fruits were used as research objects and Chinese cabbage seeds were taken as test material.The extracts were prepared by using systematic solvent extraction method and diluted into different concentrations,including 0.100,0.067,0.033,0.017 g?mL-1,which were applied to Chinese cabbage seeds in germination test.The effects of different extracts on germination and radicle length were compared in order to determine the types and parts of the germination inhibitors in Ilex asprella fruits.The results showed that endogenous germination inhibitors were found in the three parts of Ilex asprella fruits.Petroleum extracts and water extracts from the seeds significantly inhibited the germination of Chinese cabbage seeds；in the flesh,while there was a significant inhibiting effect on the germination rate of Chinese cabbage seeds under the treatment of petroleum extracts and methanol extracts,the ethyl acetate extracts reduced the radicle length of seedlings.Ethyl acetate,methanol and water extracts from the peels significantly inhibited the germination of Chinese cabbage seeds,and the petroleum ether,methanol and water extracts significantly reduced the radicle length of the seedlings.
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备注/Memo
第一作者简介：曾湘达（1992-），男，硕士研究生，研究方向为中药资源可持续利用与开发。E-mail：xdzeng2017@163.com.责任作者：何瑞（1972-），女，博士，研究员，博士生导师，现主要从事中药资源可持续利用与综合开发等研究工作。E-mail：ruihe@gzucm.edu.cn.基金项目：广东省普通高校创新团队资助项目（2016KYTD02）；广东省高水平大学建设资助项目（广中医研[2017]10号）。收稿日期：2017-09-30