[1]常怀成,倪蕾,孙涌栋,等.农杆菌介导的黄瓜遗传转化的影响因素[J].北方园艺,2018,42(02):9-14.[doi:10.11937/bfyy.20173043]
　CHANG Huaicheng,NI Lei,SUN Yongdong,et al.Factors Influencing Agrobacterium-mediated Genetic Transformation of Cucumber[J].Northern Horticulture,2018,42(02):9-14.[doi:10.11937/bfyy.20173043]
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农杆菌介导的黄瓜遗传转化的影响因素

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年02 页码: 9-14 栏目: 出版日期: 2018-01-25
Title:
Factors Influencing Agrobacterium-mediated Genetic Transformation of Cucumber
作者:
常怀成; 倪蕾; 孙涌栋; 罗未蓉
河南科技学院 园艺园林学院，河南 新乡 453003
Author(s):
CHANG Huaicheng; NI Lei; SUN Yongdong; LUO Weirong
College of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang,Henan 453003
关键词:
黄瓜; 遗传转化; 根癌农杆菌; CsEXP10
Keywords:
cucumber; genetic transformation; Agrobacterium tumefaciens; CsEXP10
DOI:
10.11937/bfyy.20173043
文献标志码:
A
摘要:
以“津优1号”黄瓜子叶节为外植体试材，研究了外植体苗态、乙酰丁香酮（AS）浓度、脱菌次数、头孢霉素（Cef）浓度及生根培养基等因素对遗传转化的影响，以期为开展黄瓜的遗传转化研究提供参考依据。结果表明：使用子叶抱合类外植体诱导分化效果最好；在预培养基和共培养基中添加100 μmol?L-1AS有利于外植体分化；用无菌水冲洗3次可有效控制农杆菌污染；选择培养基中添加300 μmol?L-1Cef，生根培养基中添加400 μmol?L-1 Cef在抑制农杆菌的同时可有效促进根系生长。
Abstract:
‘Jinyou No 1 ’ cucumber cotyledonary node explants was used as experimental material,the effects of seedling type,acetosyringone concentration （AS) in pre-culture medium and co-culture medium,washing times with sterile water,cephalosporin （Cef) concentration in select-culture medium and rooting medium on genetic transformation system were researched in order to supply reference for genetic transformation of cucumber.The results showed that various optimum parameters such as seedling type A（closed cotyledon),with 100 μmol?L-1 of AS,washed three times with sterile water,select-culture medium with 300 μmol?L-1 Cef,and rooting medium with 400 μmol?L-1 Cef were obtained with cotyledonary node explants.

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备注/Memo

第一作者简介：常怀成（1996-），男，硕士研究生，研究方向为蔬菜生理生态。E-mail：15617199956@163.com.

责任作者：罗未蓉（1981-），女，硕士，讲师，现主要从事园艺学教学与科研等工作。E-mail：wrluo2008@126.com.
基金项目：国家自然科学基金资助项目（31401860，U1204322）；河南省高校科技创新人才计划资助项目（17HASTIT040）。
收稿日期：2017-09-26

更新日期/Last Update: 2018-01-26