HU Caimin,GENG Xingmin,LIU Pan,et al.Effects of Vase Treatment of ROS-related Reagents on Postharvest Quality of Cut Lily and Rose Flowers[J].Northern Horticulture,2018,42(07):113-120.[doi:10.11937/bfyy.20172752]
活性氧相关试剂处理对月季和百合切花保鲜效果的影响
- Title:
- Effects of Vase Treatment of ROS-related Reagents on Postharvest Quality of Cut Lily and Rose Flowers
- 文献标志码:
- A
- 摘要:
- 为了明确活性氧在切花保鲜中的作用，以切花百合‘索邦’（乙烯不敏感型切花）和切花月季‘戴安娜’（乙烯敏感型切花）为试验材料，瓶插于不同浓度的硫代硫酸钠(一种自由基诱发剂，ST)、过氧化氢（H2O2）和苯甲酸钠(一种自由基清除剂，SBN)溶液中，比较各种瓶插处理液对切花观赏质量的影响。然后筛选出与对照（瓶插于蒸馏水）差异显著的处理区，对其生理指标进行分析，进一步明确各种试剂在切花保鲜中的作用机制。结果表明：自由基诱发剂在不同程度上加速了2种切花的衰老，并且浓度越高，抑制作用越显著。高浓度的ST处理加剧了丙二醛（MDA）含量的积累，加重了切花细胞膜的受损程度；而脯氨酸的积累和蛋白质含量的降低，说明ST处理加重了切花的渗透胁迫，导致切花鲜质量的迅速下降。适宜浓度的H2O2处理增大了百合和月季切花花径，减缓了切花内可溶性蛋白质含量的下降和脯氨酸、MDA的积累，延长了切花瓶插寿命。自由基清除剂SBN对百合切花保鲜无效，还在一定程度上缩短了百合切花寿命，对月季切花保鲜有一定的效果，但保鲜效果也不显著。
- Abstract:
- Lilium orential ‘Sorbonne’(ethylene insensitive cut flower) and Rosa chinensis ‘Diana’(ethylene sensitive cut flower) were used as test materials and were treated with sodium thiosulfate,hydrogen peroxide and sodium benzoate in order to study the role of reactive oxygen species (ROS) in the aging process of cut flowers.Fresh-keeping effects of each treatment were compared,and the treatments which had significant differences compared with the control (distilled water) were selected,and the physiological indexes under the treatments were measured.The results showed that 900 μmol?L-1 ST treatment decreased the content of soluble protein and increased the contents of proline and MDA,thus accelerated floral senescence;300 μmol?L-1 H2O2 treatment increased flower diameter of cut rose and lily and decreased the degradation of soluble proteins and accumulation of proline content and MDA content,prolonged vase life;100 μmol?L-1 SBN treatment had no significant effects on ornamental quality and physiological changes of cut lily and cut rose.
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备注/Memo
第一作者简介：胡才民（1990-），男，硕士研究生，研究方向为鲜切花保鲜技术。E-mail：804865995@qq.com.责任作者：耿兴敏（1976-），女，博士，教授，现主要从事园林植物种质创新与鲜切花保鲜技术等研究工作。E-mail：xmgeng@njfu.edu.cn.基金项目：江苏省高等优势学科建设工程资助项目（PAPD）。收稿日期：2017-12-04