ZHOU Chang,WANG Jinjie,FAN Haijuan,et al.Separation and Identification of Trichoderma From the Rhizosphere Soil of Prunus padus[J].Northern Horticulture,2018,42(12):88-95.[doi:10.11937/bfyy.20172427]
稠李根围木霉的分离及鉴定
- Title:
- Separation and Identification of Trichoderma From the Rhizosphere Soil of Prunus padus
- Keywords:
- Trichoderma; isolation; morphological identification; determination of ITS sequence; Prunus padus
- 文献标志码:
- A
- 摘要:
- 以黑龙江省农业科学院园艺分院稠李树（Prunus padus L.）的根围土壤为试验材料，采用孟加拉红平板分离法及Gams&Bissett（1998）形态分类系统与Kullnig-Gradinger等(2002)的分子分类观点的鉴定方法，研究了稠李树（Prunus padus L.）的根围土壤中木霉（Trichoderma）的种类、数量及多样性状况，以期发现其根围土壤中的优势木霉菌。结果表明：共分离得到哈茨木霉（T.harzianum）25株，说明稠李根围木霉菌丰富但是种类单一，该研究结果为“稠李专用木霉菌肥”的制备提供菌种资源。
- Abstract:
- The materials of the rhizosphere soil of bird cherry (Prunus padus L.) from the Academy of Agricultural Sciences Institute of Horticulture in Harbin and isolation methods of Rose Bengal medium and plate separation as well as identification methods of morphological classification presented by Gams&Bissett（1998） and molecular classification presented by Kullnig-Gradinger et al.(2002) were used to research the identity,number and variety of Trichoderma existed in the rhizosphere soil of bird cherry.The purpose of the research was to find the dominant species of Trichoderma in the rhizosphere soil of bird cherry.The results showed that 25 Trichoderma Harzianum strains isolated from the sample,which means there were large quality but only one species of Trichoderma spp.around the bird cherry root.The research results would provide Trichoderma resource for special Trichoderma biofertilizer of the bird cherry.
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备注/Memo
第一作者简介：周畅（1994-），女，辽宁营口人，硕士研究生，研究方向为木霉剂型。E-mail：2413928428@qq.com.责任作者：刘志华（1976-），女，博士，副教授，现主要从事木霉菌种收集与保藏及木霉菌肥与剂型的研发及木霉分子机理等研究工作。E-mail：LZHNEFU@126.com.基金项目：中央高校基本科研业务专项E类预研资助项目（2572015EY01）；东北林业大学青年基金资助项目（09039）。收稿日期：2017-10-12