[1]叶胜兰,雷光宇.叶面喷施铁、锌肥对黄土丘陵区“梨枣”抗氧化性的影响[J].北方园艺,2017,41(24):48-53.[doi:10.11937/bfyy.20172227]
　YE Shenglan,LEI Guangyu.Effects of Iron and Zinc Fertilizers on Antioxidant Activity of ‘Pear-jujube’ in Loess Hilly Region[J].Northern Horticulture,2017,41(24):48-53.[doi:10.11937/bfyy.20172227]
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叶面喷施铁、锌肥对黄土丘陵区“梨枣”抗氧化性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年24 页码: 48-53 栏目: 出版日期: 2017-12-25
Title:
Effects of Iron and Zinc Fertilizers on Antioxidant Activity of ‘Pear-jujube’ in Loess Hilly Region
作者:
叶胜兰; 雷光宇
陕西省土地工程建设集团有限责任公司,陕西地建土地工程技术研究院有限责任公司,国土资源部退化及未利用土地整治重点实验室，陕西 西安 710075
Author(s):
YE Shenglan; LEI Guangyu
Shaanxi Provincial Land Engineering Construction Group Co.,Ltd./Institute of Land Engineering and Technology,Shaanxi Provincial Land Engineering Construction Group Co.,Ltd./Key Laboratory of Degraded and Unused Land Consolidation Engineering,the Ministry of Land and Resources,Xi′an,Shaanxi 710075
关键词:
“梨枣”; 铁、锌肥; 多酚; 抗氧化性
Keywords:
‘pear-jujube’; iron and zinc fertilizer; polyphenols; antioxidant
DOI:
10.11937/bfyy.20172227
文献标志码:
A
摘要:
选择长势均一、生长良好的8年生山地矮化密植“梨枣”为研究对象，设置不同浓度铁、锌微肥处理对“梨枣”叶片进行叶面喷施，分析了“梨枣”中维生素C、总黄酮含量、酶含量的变化以及“梨枣”的不同浓度多酚对羟基自由基、1,1-二苯基-2-三硝基苯肼（DPPH?）及过氧化氢的清除率，以探索铁、锌肥对黄土丘陵区“梨枣”抗氧化性的影响。结果表明：不同处理影响“梨枣”中维生素C含量，并显著提高了总黄酮含量，其中以0.6% FeSO4+0.3% ZnSO4（L3）处理的含量最高，达到2.86 mg?g-1和3.02 mg?g-1，分别较CK提高了21.8%和105.4%，且均与CK达到显著差异（P<0.05）。不同施肥处理有效降低枣果中抗坏血酸氧化酶及多酚氧化酶的活性。0.6% FeSO4+0.3% ZnSO4处理的抗坏血酸氧化酶活性表现最低，为氧化抗坏血酸 0.069 mg?g-1?min-1FW，较CK降低了75.1%；多酚氧化酶活性以L3处理的多酚氧化酶活性表现最低，为氧化抗坏血酸0.146 mg?g-1?min-1FW，较CK降低了42.0%。不同处理“梨枣”的多酚能有效清除羟基自由基、DPPH?以及过氧化氢，其中以L3处理的效果最为显著，抗氧化性效果最优。
Abstract:
In order to explore the effect of iron and zinc fertilizer on the antioxidant activity of ‘pear-jujube’ in hilly loess hilly region,8-year-old mountain dwarfing ‘pear-jujube’ with uniform and well growth were used as test objects,the effects of different concentrations of iron and zinc fertilizer on vitamin C,total flavonoid content and enzyme content were studied.The results showed that different treatments affected the content of vitamin C in ‘pear-jujube’ and significantly increased the contents of vitamin C and total flavonoids.The content of 0.6% FeSO4+0.3% ZnSO4 was 2.86 mg?g-1 and 3.02 mg?g-1,which was 21.8% and 105.4% higher than CK respectively (P<0.05).The different fertilization treatments effectively reduced the activity of ascorbic acid oxidase and polyphenol oxidase in ‘pear-jujube’.Treatment of 0.6% FeSO4+0.6% ZnSO4 showed the highest activity of ascorbic acid oxidase,which was 0.069 mg?g-1?min-1 FW,which was 75.1% lower than that of CK.The activity of polyphenol oxidase was the lowest in 0.146 mg?g-1?min-1FW,which was 42.0% lower than CK.The polyphenols treated with different treatments could effectively remove hydroxyl radicals,DPPH? and hydrogen peroxide,among which the effect of treatment with 0.6% FeSO4+0.3% ZnSO4 was the most significant.Comprehensive analysis of the antioxidant effect of the treatment with 0.6% FeSO4+0.3% ZnSO4 was optimal.

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备注/Memo

第一作者简介：叶胜兰（1986-），女，硕士，研究方向为植物营养及土地整治。E-mail：552769044@qq.com.

基金项目：陕西省自然科学基金资助项目（2016KCT-23）。
收稿日期：2017-07-24

更新日期/Last Update: 2017-12-28