[1]王吉腾,赵世恒,徐丽,等.板栗针叶小爪螨体内Wolbachia的wsp基因序列测定与分析[J].北方园艺,2018,42(01):35-39.[doi:10.11937/bfyy.20172043]
　WANG Jiteng,ZHAO Shiheng,XU Li,et al.Sequencing and Sequence Analysis of wsp Gene of Wolbachia inOligonychus ununguis Jacobi on Chestnut[J].Northern Horticulture,2018,42(01):35-39.[doi:10.11937/bfyy.20172043]
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板栗针叶小爪螨体内Wolbachia的wsp基因序列测定与分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年01 页码: 35-39 栏目: 出版日期: 2018-01-10
Title:
Sequencing and Sequence Analysis of wsp Gene of Wolbachia inOligonychus ununguis Jacobi on Chestnut
作者:
王吉腾1; 赵世恒2; 徐丽3; 颜显辉1; 王萍1; 王春鹏1
1.荣成出入境检验检疫局，山东 荣成 264300;2.北京市通州区农产品质量检验检测站，北京 101105;3.青岛出入境检验检疫局，山东 青岛 266000
Author(s):
WANG Jiteng1; ZHAO Shiheng2; XU Li3; YAN Xianhui1; WANG Ping1; WANG Chunpeng1
1.Rongcheng Entry-exit Inspection and Quarantine Bureau，Rongcheng,Shandong 264300；2.Beijing Tongzhou Agricultural Products Quality Inspection Station，Beijing 101105；3.Qingdao Entry-exit Inspection and Quarantine Bureau，Qingdao,Shandong 266000
关键词:
针叶小爪螨; Wolbachia; wsp基因; 序列分析
Keywords:
Oligonychus ununguis; Wolbachia; wsp gene; sequence analysis
DOI:
10.11937/bfyy.20172043
文献标志码:
A
摘要:
以板栗针叶小爪螨为试虫,采用PCR扩增法，应用Wolbachia的wsp基因特异引物对我国部分板栗产区（北京密云南达峪村、山东泰安赵峪村与山东临沂柳条峪村）板栗园针叶小爪螨进行了检测，以期为通过分子生物学手段检疫鉴定针叶小爪螨提供参考依据。结果表明：在北京密云南达峪村与山东泰安赵峪村板栗园针叶小爪螨体内扩增出593 bp Wolbachia的wsp基因；在山东临沂柳条峪村板栗园针叶小爪螨体内未检测到Wolbachia特征基因片段。利用所测序列和其它已发表的序列建立系统树，发现针叶小爪螨体内Wolbachia属于B大组的Epo小组。
Abstract:
Oligonychus ununguis was used as test insect.Specific primers derived from the wsp gene sequence were used to amplify DNA of Wolbachia from part of chestnut orchards（Miyun Beijing,Tai′an Shandong and Linyi Shandong chestnut orchards） of Oligonychus ununguis in China by polymerase chain reaction （PCR)，in order to supply reference for detecting Oligonychus ununguis by molecular biological method.The results showed that the 596 bp products of wsp gene were observed after amplification of extracts from chestnut orchards （Nandayu village of Beijing and Zhaoyu village of Shandong Province) of Oligonychus ununguis，but in other chestnut orchards （Liutiaoyu village of Shandong Province)，this gene was not found.By analyzing the wsp gene sequences of Wolbachia in Oligonychus ununguis by constituting the phylogenetical tree，it was found that they were identical with the wsp gene sequence of some strains of Epo group in B supergroup of Wolbachia.

参考文献/References:

参考文献

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[1]张 美 淑,金 大 勇,刘 继 生.针叶小爪螨的发生规律及药剂防治[J].北方园艺,2007,31(12):0.[doi:10.11937/bfyy.2007012095]
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备注/Memo

第一作者简介：王吉腾（1982-），男，硕士，农艺师，现主要从事食品农产品安全检测监管等研究工作。E-mail:wangjiteng2008@126.com.

基金项目：山东检验检疫局科研计划资助项目（SK201717）。
收稿日期：2017-08-03

更新日期/Last Update: 2018-01-12