[1]公华锐,骆洪义,亓艳艳,等.不同硝铵比对基质栽培番茄氮素代谢关键酶及其氮素利用效率的影响[J].北方园艺,2017,41(24):7-16.[doi:10.11937/bfyy.20172008]
　GONG Huarui,LUO Hongyi,QI Yanyan,et al.Effects of NO3--N/NH4+-N Ratio on Enzyme Activity in Nitrogen Assimilation and Nitrogen Efficiency of Tomato Cultivated in Organic Substrate[J].Northern Horticulture,2017,41(24):7-16.[doi:10.11937/bfyy.20172008]
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不同硝铵比对基质栽培番茄氮素代谢关键酶及其氮素利用效率的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年24 页码: 7-16 栏目: 出版日期: 2017-12-25
Title:
Effects of NO3--N/NH4+-N Ratio on Enzyme Activity in Nitrogen Assimilation and Nitrogen Efficiency of Tomato Cultivated in Organic Substrate
作者:
公华锐; 骆洪义; 亓艳艳; 庞晓燕
山东农业大学 资源与环境学院，山东 泰安 271000
Author(s):
GONG Huarui; LUO Hongyi; QI Yanyan; PANG Xiaoyan
College of Resources and Environment,Shandong Agricultural University,Tai′an,Shandong 271000
关键词:
番茄; 基质栽培; 硝态N; 铵态N; N素代谢; N素利用
Keywords:
tomato; substrate cultivation; NO3--N; NH4+-N; N assimilation; N efficiency
DOI:
10.11937/bfyy.20172008
文献标志码:
A
摘要:
以春冬茬番茄为研究对象，采用基质盆栽试验，研究等量氮（N素）供应下不同硝铵态氮比例对番茄生长过程中N素代谢、吸收及其利用效率的影响。结果表明：在等量氮素供应的条件下，与CK（全硝态N）相比，处理T1（5%铵态N）、T2（10%铵态N）、T3（15%铵态N）、T4（25%铵态N）的番茄叶片中N素代谢关键酶及地上部硝态N含量在生长发育过程中，随着营养液中铵态N比例的增加而降低，叶片中谷氨酰胺还原酶活性随着铵态N比例的增加而提高，处理T4的番茄叶片硝酸还原酶活性及硝酸盐含量相对于CK分别下降了45.5%与17.8%，谷氨酰胺还原酶活性相对于CK提高了50.2%。不同硝铵比通过调节氮素关键酶活性，进而对番茄氮素的吸收、分配等产生影响。适量增加铵态N比例能有效促进番茄对N素的吸收，提高番茄产量，但当铵态N比例增加至T4处理水平时，番茄产量及N素利用率均有显著的下降。T3处理番茄产量相对于T4处理提高了21.2%、N素偏生产力高于T4处理47.09 g?g-1，N素收获指数达到0.48 g?g-1，同时高于其它处理，是该试验的最佳处理。
Abstract:
In the present research,winter-crossing greenhouse tomato was taken as experimental plants by pot containing substrate,the effects of different NH4+-N/NO3--N ratios related to N assimilation and uptake,efficiency of tomato were studied.By the equivalent N supply,compared to CK(0% NH4+-N),at T1(5% NH4+-N),T2(10% NH4+-N),T3(15% NH4+-N),T4(20% NH4+-N) the NR activity and NO3--N contents of tomato plants decreased with the increased proportion of NH4+-N within total N,the GS activity of tomato increased with the increased proportion of NH4+-N in nutrient solution.The enzyme activity responsible for N assimilation was regulated effectively by moderately increased proportion of NH4+-N,and the absorption and efficiency of N by tomato plants was promoted as well.When the NH4+-N proportion raised at T4,the field and N efficiency decreased significantly,and at T3 NH4+-N proportion,the field and N efficiency were better than the other treatments.Thus,based on the experimental results,it was suggested that the NH4+-N proportion of treatment T3 would be the optimal one.

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备注/Memo

第一作者简介：公华锐（1991-），男，硕士研究生，研究方向为无土栽培及其营养机理。E-mail:gong187@126.com.

责任作者：骆洪义（1965-），男，教授，研究方向为无土栽培技术。E-mail:hot68168@163.com.
基金项目：山东省自主创新及成果转化专项资助项目（2014ZZCX070402）。
收稿日期：2017-07-26

更新日期/Last Update: 2017-12-28