[1]王殿东,李莹.设施温室土壤线虫群落物种组成和营养类群分析[J].北方园艺,2018,42(01):114-118.[doi:10.11937/bfyy.20171729]
　WANG Diandong,LI Ying.Diversity and Trophic Group of Soil Nematode Habitat in Greenhouse[J].Northern Horticulture,2018,42(01):114-118.[doi:10.11937/bfyy.20171729]
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设施温室土壤线虫群落物种组成和营养类群分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年01 页码: 114-118 栏目: 出版日期: 2018-01-10
Title:
Diversity and Trophic Group of Soil Nematode Habitat in Greenhouse
作者:
王殿东1; 李莹2
1.长江师范学院 生命科学与技术学院，重庆 408011；2.北华大学 林学院，吉林 吉林 132013
Author(s):
WANG Diandong1; LI Ying2
1.College of Life Science and Technology，Yangtze Normal University,Chongqing 408011；2.Forestry College，Beihua University,Jilin,Jilin 132013
关键词:
土壤线虫; 18S rDNA克隆文库; 成熟度指数; 植物寄生线虫成熟度; 根结线虫
Keywords:
soil nematode; 18S rDNA clone library; MI; PPI; root knot nematode
DOI:
10.11937/bfyy.20171729
文献标志码:
A
摘要:
以种植12年的设施温室土壤线虫群落为研究对象，采用土壤线虫18S rDNA克隆文库方法，研究了土壤线虫物种多样性指数、植物寄生线虫成熟度以及自由生活线虫成熟度等土壤线虫群落结构特征，以期揭示土壤健康程度。结果表明：设施蔬菜土壤线虫以食细菌线虫为优势类群，占59.4%；植物寄生线虫比例次之，占34.9%。在属水平上，以根结线虫为优势类群，比例为31.5%，其次为拟小杆属线虫，比例为28.8%。设施温室土壤线虫的成熟度指数和植物寄生线虫成熟度指数分别为1.86和1.11。总体来看，设施温室土壤中植物寄生线虫比例较高，这反映出设施温室土壤受到人为干扰较大，且线虫营养类群结构失调，土壤健康状况不良。
Abstract:
In order to analyze the characters of soil nematode community in greenhouse 12 years history,and to assess the status of soil health in greenhouse,the community of soil nematode was investigated to reveal the diversity of soil nematode community,plant parasite index （PPI) and mature index （MI) by soil nematode 18S rDNA clone library.The results showed that the dominant soil nematode group of 12 years greenhouse were bacterial-feeding nematodes with percents of 59.4%,followed by plant parasitic nematode with percent of 34.9%.At genus level,Meloidogyne was the predominant nematode groups represented 31.5%,followed by Rhabditoides with 28.8%.Moreover,the PPI and MI of soil nematode community in 12 years greenhouse were 1.11 and 1.86,respectively.In a conclusion,plant parasitic nematode possessed high percentage in soil nematode community,suggesting that soil was disturbed by farm operation and structure of soil nematode community disorder.On the whole,health of soil in greenhouse was abnormal.

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备注/Memo

第一作者简介：王殿东（1979-），男，博士，副教授，现主要从事植物土传病害等研究工作。E-mail：ddwangwill@163.com.

基金项目：重庆市教委资助项目（KJ1401213）。
收稿日期：2017-08-07

更新日期/Last Update: 2018-01-12