PEI Dongli,ZHU Xiaoqin,WANG Shunan,et al.Molecular Identification of Powdery Mildew of Medicinal Herb Galium aparine[J].Northern Horticulture,2018,42(09):149-152.[doi:10.11937/bfyy.20171623]
草本药用植物猪殃殃白粉菌分子生物学鉴定
- Title:
- Molecular Identification of Powdery Mildew of Medicinal Herb Galium aparine
- Keywords:
- Galium aparine; powdery mildew; ITS sequences; morphological identification; etiological identification; molecular systematics
- 文献标志码:
- A
- 摘要:
- 以河南商丘地区草本药用植物猪殃殃白粉菌为试材，采用分子系统学方法研究了其形态学、致病性鉴定、分子生物学鉴定及其系统进化关系，以期为白粉病的防治提供理论依据。结果表明：猪殃殃白粉菌着生于叶的两面及茎上，随着病原菌的发展，蔓延至覆盖整个叶子表面。显微分析结果表明，病原菌分生孢子梗直立，(100~260)μm×(10~12)μm，产生2~3个未成熟的分生孢子。足细胞(28~65)μm×(9~11)μm，紧邻着2~3个短细胞。分生孢子桶-柱形，(22~42)μm×(15~20)μm。对其核糖体DNA内转录间隔区（ITS）序列进行PCR扩增，测序获得632 bp序列，GenBank登陆号为KX396590。经MEGA 5.0软件分析，其与奥隆特高氏白粉菌Golovinomyces orontii ITS 序列AB430813序列聚为一枝，而来自其它属种白粉菌的5条ITS序列亲缘关系较远。河南商丘地区的猪殃殃白粉菌为奥隆特高氏白粉菌G.orontii。
- Abstract:
- Galium aparine powdery mildew detected in Shangqiu of Henan was used as experiment material，morphological，etiological and molecular systematic identification，and phylogenetic evolution relatiognship of the powdery mildew were identified by molecular systematics methods，in order to provide a theoretical basis for its prevention and treatment.The results showed that the powdery mildew was grown on two sides of leaves and stems，then covering the whole leaf surface with the pathogen progress.The conidiophores were erected，(100-260)μm×(10-12)μm，with 2-3 immature spores.The foot cells were (28-65)μm×(9-11)μm，next to 2-3 short cells.The spores were cylindrical，(22-42)μm×(15-20)μm.Internal transcribed spacer (ITS) sequence of the pathogen ribosomal DNA was amplified by PCR and sequenced to obtain the 632 bp with the GenBank accession number KX396590 and sequence analysis by MEGA 5.0 software revealed this ITS sequence and one sequence of Golovinomyces orontii (GenBank accession number AB430813) were gathered together，distantly related to the ITS sequence of other five species in the same genus.The results showed that pathogen of G.aparine powdery mildew in Shangqiu was G.orontii.
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备注/Memo
第一作者简介：裴冬丽（1971-），女，博士，教授，现主要从事植物抗病分子生物学教学与科研等工作。E-mail：peidongli@126.com.责任作者：李成伟（1972-），男，博士，教授，博士生导师，现主要从事植物分子遗传学等研究工作。E-mail：lichengweiwau@hotmail.com.基金项目：国家自然科学基金资助项目（31571997）；河南省教育厅资助项目（15A180019，15A210045）。收稿日期：2017-12-27