[1]吴晓晶,李程亮,周园园,等.无机化合物对北方根结线虫二龄幼虫存活的影响[J].北方园艺,2017,41(24):53-59.[doi:10.11937/bfyy.20171600]
　WU Xiaojing,LI Chengliang,ZHOU Yuanyuan,et al.Effects of Inorganic Compounds on Survival of Meloidogyne hapla Juveniles[J].Northern Horticulture,2017,41(24):53-59.[doi:10.11937/bfyy.20171600]
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无机化合物对北方根结线虫二龄幼虫存活的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年24 页码: 53-59 栏目: 出版日期: 2017-12-25
Title:
Effects of Inorganic Compounds on Survival of Meloidogyne hapla Juveniles
作者:
吴晓晶1; 李程亮1; 2; 周园园1; 段玉玺1; 陈立杰1
1.沈阳农业大学 植物保护学院，北方线虫研究所，辽宁 沈阳 110866；
2.西北农林科技大学 植物保护学院，陕西 杨凌 712100
Author(s):
WU Xiaojing1; LI Chengliang1; 2; ZHOU Yuanyuan1; DUAN Yuxi1; CHEN Lijie1
1.Plant Protection College，Shenyang Agricultural University/Nematological Institute of Northern China,Shenyang,Liaoning 110866;2.College of Plant Protection,Northwest A & F University,Yangling,Shaanxi 712100
关键词:
北方根结线虫; 无机化合物; 死亡率; pH
Keywords:
Meloidogyne hapla; inorganic compound; mortality; pH
DOI:
10.11937/bfyy.20171600
文献标志码:
A
摘要:
以北方根结线虫(Meloidogyne hapla)为靶标材料，采用离体触杀的方法，测定了8种无机化合物对北方根结线虫存活的影响，研究了北方根结线虫对无机化合物和pH的适应性，以期为北方根结线虫的生态防治提供参考依据。结果表明：不同的无机化合物对北方根结线虫J2的影响不同；同一种化合物在不同浓度下对J2的致死率也不同，并且随着无机化合物浓度的增加，J2的死亡率上升。其中，NH4HCO3、FeCl3?6H2O、CuCl2?2H2O、CuSO4?5H2O这4种化合物对J2作用最为敏感，半致死浓度LC50分别是0.005 957、0.000 771、0.000 159、0.000 161 mol?L-1，即为敏感的化合物，而其它化合物只在高浓度下致死效果明显。通过萌发试验，进一步验证了这4种无机化合物的LC50和LC90对番茄种子的影响，结果表明，其对种子的萌发均无影响，但对根的伸长有一定的抑制作用。同时通过pH试验，明确了北方根结线虫J2适宜存活的pH范围在4~12。
Abstract:
Meloidogyne hapla was used as the target material to study the effects of eight compounds on the survival of M.hapla J2 at different concentrations in vitro,in order to provide basic knowledge for ecological control.The results showed that different inorganic compounds had different effect on M.hapla;and the lethality of the same compounds at different concentration was different too,with the increase of concentration,the mortality of J2 increased.While NH4HCO3,FeCl3?6H2O,CuCl2?2H2O,CuSO4?5H2O were the most sensitive among 8 compounds,and the LC50 were respectively 0.005 957，0.000 771，0.000 159，0.000 161 mol?L-1.All compounds at LC50 and LC90 had no inhibitory effect on tomato seed germination except for roots elongation in the latter stage.And the study found that the suitable pH range for survival of M.hapla J2 was 4-12.

参考文献/References:

[1]KOENNING S,OVERSTREET C,NOLING J,et al.Survey of crop losses in response to phytoparasitic nematodes in the United States for 1994[J].Journal of nematology,1999,31(4S):587.

[2]PERRY R N,MOENS M,STARR J L.Root-knot nematodes[M].USA:CABI,2009.
[3]ELLING A A.Major emerging problems with minor Meloidogyne species[J].Phytopathology,2013,103(11):1092-1102.
[4]谢联辉.普通植物病理学[M].北京:科学出版社,2013.
[5]刘维志.植物病原线虫学[M].北京:中国农业出版社,2000.
[6]CASTRO C,BELSER N,MCKINNEY H,et al.Strong repellency of the root knot nematode,Meloidogyne incognita by specific inorganic ions[J].Journal of Chemical Ecology,1990,16(4):1199-1205.
[7]OKA Y,PIVONIA S.Use of ammonia-releasing compounds for control of the root-knot nematode Meloidogyne javanica[J].Nematology,2002,4(1):65-71.
[8]OKA Y,PIVONIA S.Effect of a nitrification inhibitor on nematicidal activity of organic and inorganic ammonia-releasing compounds against the root-knot nematode Meloidogyne javanica[J].Nematology,2003,5(4):505-513.
[9]OKA Y,TKACHI N,SHUKER S,et al.Laboratory studies on the enhancement of nematicidal activity of ammonia-releasing fertilisers by alkaline amendments[J].Nematology,2006,8(3):335-346.
[10]SVOLY Z,NAGY P,VARGA G,et al.A novel method for investigation of uptake and distribution of polluting microelements and nanoparticles in soil-inhabiting nematodes[J].Microchemical Journal,2013,110：110558-110567.
[11]B〖AKU-〗DA V,〖AKCˇ〗EPULYT〖AKE?〗-RAKAUSKIEN〖AKE?〗 R.The effects of α-solanine and zinc sulphate on the behaviour of potato cyst nematodes Globodera rostochiensis and G.pallida[J].Nematology,2015,17(9):1105-1111.
[12]OKA Y,TKACHI N,MOR M.Phosphite inhibits development of the nematodes Heterodera avenae and Meloidogyne marylandi in cereals[J].Phytopathology,2007,97(4):396-404.
[13]KHAN M,KHAN M.Effects of simulated acid rain and root-knot nematode on tomato[J].Plant Pathology,1994,43(1):41-49.
[14]段玉玺,白春明,陈立杰,等.无机化合物对南方根结线虫行为的影响[J].植物保护学报,2009(6):537-544.
[15]韩冰洁,朱晓峰,王媛媛,等.三种化合物处理对南方根结线虫生长发育及致病能力的影响[J].植物保护学报,2015(2):251-257.
[16]白春明,段玉玺,陈立杰,等.铵类化合物对南方根结线虫2龄幼虫存活的影响[J].植物保护,2009(1):74-78.
[17]伏召辉,杜超,仵均祥.温湿度及酸碱度对南方根结线虫生长发育的影响[J].北方园艺,2012(6):137-140.
[18]陈剑东,韩琪.水体重金属对秀丽隐杆线虫Caenorhabditis elegans的急性毒性研究[J].农业科技与信息,2016(31):43-44.
[19]张守申.二氧化钛纳米与重金属Cd离子相互作用降低Cd2+对秀丽隐杆线虫的毒性[D].泰安:山东大学,2014.
[20]宋少娟,郭亚平,阴琨,等.秀丽小杆线虫耐铜性筛选及其耐铜机制探讨[J].四川动物,2008,27(5):832-834.
[21]XIONG J,ZHOU Q,LUO H,et al.Systemic nematicidal activity and biocontrol efficacy of Bacillus firmus against the root-knot nematode Meloidogyne incognita[J].World Journal of Microbiology and Biotechnology,2015,31(4):661-667.
[22]RADWAN M,FARRAG S,ABU-ELAMAYEM M,et al.Biological control of the root-knot nematode,Meloidogyne incognita on tomato using bioproducts of microbial origin[J].Applied Soil Ecology,2012,56：5658-5662.
[23]LAMOV〖AKSˇ〗EK J,UREK G,TRDAN S.Biological control of root-knot nematodes (Meloidogyne spp.):Microbes against the pests/BIOTI〖AKCˇ〗NO ZATIRANJE OGOR〖AKCˇ〗IC KORENINSKIH 〖AKSˇ〗I〖AKSˇ〗K (Meloidogyne spp.):MIKROORGANIZMI PROTI 〖AKSˇ〗KODLJIVCEM[J].Acta Agriculturae Slovenica,2013,101(2):263-275.

备注/Memo

第一作者简介：吴晓晶（1989-），女，福建三明人，博士研究生，研究方向为植物线虫学。E-mail:wuxiaojing0106@163.com.

责任作者：段玉玺（1964-）,男，博士，教授，博士生导师，现主要从事植物病理学和线虫学的教学与科研等工作。E-mail：duanyx6407@163.com.
基金项目：农业部公益性行业科研专项资助项目（201103018，200903040-03）；国家自然科学基金资助项目（31471748）。
收稿日期：2017-07-24

更新日期/Last Update: 2017-12-28