WANG Xue,LI Guan.Construction of Expression Vector Knocking Out ACC Synthase Gene in Melon by CRISPR-Cas9 System[J].Northern Horticulture,2017,41(12):114-118.[doi:10.11937/bfyy.201712027]
CRISPR-Cas9系统敲除甜瓜ACC合成酶基因表达载体的构建
- Title:
- Construction of Expression Vector Knocking Out ACC Synthase Gene in Melon by CRISPR-Cas9 System
- 关键词:
- CRISPR-Cas9; 基因敲除; ACC合成酶
- Keywords:
- CRISPR-Cas9; gene knockout; ACC synthase gene
- 文献标志码:
- A
- 摘要:
- 以甜瓜品种“老汉瓜”为试材，采用CRISPR-Cas9基因组编辑技术，研究了构建CRISPR-Cas9系统敲除甜瓜ACC合成酶基因表达载体的方法，以期获得高效的敲除甜瓜基因的基因组编辑技术。结果表明：以甜瓜ACC合成酶的基因设计出引物后，将其构建到pP1C.4载体上，用热激法将连接产物转化到大肠杆菌中，筛选的阳性克隆测序验证为最终载体。
- Abstract:
- The CRISPR-Cas9 genome editing techniques of melon，was constructed a vector which knockout ACC synthase gene by CRISPR-Cas9 system,and then the high efficient genome editing technique was obtained which could knockout melon genes.The results showed that based on the ACC synthase gene of melon to designed primers,and then they were builded to the pP1C.4 vector.The connect product was transformed into escherichia coli by heat shock method.Finally,a few positive clones were selected,they were final vector.
参考文献/References:
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备注/Memo
第一作者简介：王雪（1992-），女，硕士研究生，研究方向为植物学。E-mail：870432310@qq.com.责任作者：李冠（1949-），男，教授，博士生导师，现主要从事植物生理生化与分子生物学教学等工作。E-mail：guanli@xju.edu.cn.