[1]金忠民,赵婧佟,温昱晨,等.耐铅镉菌株的筛选及其生长条件的优化[J].北方园艺,2017,41(24):65-71.[doi:10.11937/bfyy.20171122]
　JIN Zhongmin,ZHAO Jingtong,WEN Yuchen,et al.Screening Strains Resistant of Lead and Cadmium and Growth Conditions Optimization[J].Northern Horticulture,2017,41(24):65-71.[doi:10.11937/bfyy.20171122]

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耐铅镉菌株的筛选及其生长条件的优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷期数: 2017年24 页码: 65-71 栏目: 出版日期: 2017-12-25

Title:: Screening Strains Resistant of Lead and Cadmium and Growth Conditions Optimization

作者:: 金忠民; 赵婧佟; 温昱晨; 聂文军; 刘丽杰; 齐齐哈尔大学生命科学与农林学院，黑龙江齐齐哈尔 161006

Author(s):: JIN Zhongmin; ZHAO Jingtong; WEN Yuchen; NIE Wenjun; LIU Lijie; College of Life Science and Agriculture and Forestry,Qiqihar University,Qiqihar,Heilongjiang 161006

关键词:: 耐Pb²⁺/Cd²⁺菌株; 分离鉴定; 生物学特性

Keywords:: Pb²⁺/Cd²⁺ resistant strain; isolation and identification; biological characteristics

DOI:: 10.11937/bfyy.20171122

文献标志码:: A

摘要:: 以黑龙江扎龙湿地土壤为试材，采用形态特征观察、生理生化特性研究和16S rDNA序列系统发育分析的方法，研究了培养温度重金属、浓度、NaCl浓度、pH、抗生素种类和氧气对菌株生长的影响，以期为利用耐铅、镉菌株治理重金属污染提供参考依据。结果表明：筛选出的耐金属菌株初步鉴定其为Burkholderia sp.，菌株最适温度为30 ℃，最适pH为7，可以在含盐量<6%的培养基上生长。随着NaCl浓度的升高，该菌株生长受到抑制。菌株可能属于好氧菌。对于其它重金属，在低浓度含Cu2+培养基生长，高浓度抑制，对Cr6+不具耐性。该菌株能在含有不同低浓度的抗生素环境下生长，高浓度时抑制生长。

Abstract:: This experiment is based on the soil of Zalong wetland in Heilongjiang Province,using the method of state characteristic observation,physiological and biochemical characteristics and the development analysis of 16S rDNA sequence systems,the effects of temperature,heavy metal concentration,NaCl concentration,pH,antibiotic type and oxygen on the growth of strain were studied,in order to provide reference for the use of lead-resistant and cadmium strains to control heavy metal pollution.The results showed that a strain resistant to heavy metals was screened and initially identified as Burkholderia sp,the optimum temperature was 30 ℃ and the optimum pH was 7.It could be grown on medium with salt content <6%.With the increase of NaCl concentration,the growth of the strain was inhibited.The strain might belong to aerobic bacteria.For other heavy metals,in the low concentration of Cu2+ containing medium growth,high concentration inhibition,not resistant to Cr6+.The strain could grow in an environment containing different concentrations of antibiotics,high concentrations were inhibited when grew.

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备注/Memo

第一作者简介：金忠民（1968-），女，博士，副教授，研究方向为污染生态学与植物生理及遗传多样性。E-mail:yyy6768@163.com.

收稿日期：2017-07-18

更新日期/Last Update: 2017-12-28