GENG Chenchen,ZHANG Junxiang,LI Qiang,et al.Determination of Ten Mono-phenols in Dry Red Wines of Ningxia by HPLC[J].Northern Horticulture,2017,41(11):140-144.[doi:10.11937/bfyy.201711028]
基于高效液相色谱法的宁夏葡萄酒中十种酚类物质的测定
- Title:
- Determination of Ten Mono-phenols in Dry Red Wines of Ningxia by HPLC
- Keywords:
- grape wine; mono-phenol; HPLC
- 文献标志码:
- A
- 摘要:
- 以宁夏贺兰山东麓10个葡萄酒样品为试材，采用了HPLC法同时测定陈酿葡萄酒中没食子酸、水杨酸等10 种单体酚，并通过样品回收率和精密度的测试，确定了最佳色谱条件。结果表明：UltraⅡ-C18色谱柱（250 mm×4.6 mm，5 μm），流动相 A 为水∶乙酸(98∶2)(V/V)，流动相 B 为乙腈，梯度洗脱，流速为1.0 mL?min-1，检测波长为280 nm，在此条件下各物质在25 min内得到了良好的分离，各物质的含量和峰面积均有较好的线性关系(r>0.999)，同时回收率较高(>80%)，重复性好，具有定量准确、时间短等特点。10个葡萄酒样品中均能检测到这10种物质，且含量各不相同；其中没食子酸、儿茶素含量均较高。
- Abstract:
- Ten red wine samples from Helan Mountain in Ningxia were used as test materials.Simultaneous determination of 10 mono-phenols in wines by HPLC was studied.The results showed that chromatographic separation was performed by UltraⅡ-C18 precolumn (250 mm × 4.6 mm,5 μm).A gradient elution consisting of 〖JP〗solvent A (water∶acetic acid，98∶2，V/V) and solvent B (acetonitrile) was applied with flow rate at 1.0 mL?min-1 and detection wavelength at 280 nm.Under this chromatographic condition，the calibrations curves were linear with r>0.999 and recoveries of several mono-phenols>80%.The results showed that 10 mono-phenols were found in ten wines.Gallic acid and catechin content were higher than that of all the mono-phenols.
参考文献/References:
[1]REVILLA E,GARCLA-BENEYTEZ E,CABELLO F,et al.Value of high-performance liquid chromatographic analysis of anthocyanins in the differentiation of red grape cultivars and red wines made from them[J].J Chromatogr A,2001,915:53-60. [2]ESCRIBANO-BAILN M T,SANTOS-BUELGA C,RIVAS-GONZALO J C.Anthocyanins in cereals[J].Journal of Chromatography A,2004,1054:129-141. [3]REIN M.Copigmentation reactions and color stability of berry anthocyanins[D].Helsinki:University of Helsinki,2005. [4]HE J.Absorption,excretion,and transformation of individual anthocyanins in rats[D].Maryland:University of Maryland,2004. [5]王霞,王恭堂.橡木片的选择与使用方法[J].中外葡萄与葡萄酒,2002(4):44-46. [6]李华.橡木桶与葡萄酒[J].中外葡萄与葡萄酒,2002(1):49-53. [7]成宇峰,张振文,岳泰新,等.HPLC同时检测葡萄酒中10种单体酚的方法[J].食品科学,2008(4):287-290. [8]孙翔宇.单品种葡萄酒、商业葡萄酒酚类物质分析及发酵时去籽时间对葡萄酒中酚类物质含量影响[D].西安:陕西师范大学,2013. [9]陈建业.葡萄酒中酚酸及葡萄果实苯丙烷类代谢途径研究[D].北京:中国农业大学,2005. [10]韩国民,陈锋,侯敏,等.葡萄酒中14种单体酚的高效液相色谱测定[J].食品科学,2011,32(2):180-183.
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备注/Memo
第一作者简介：耿晨晨(1990-)，女，硕士研究生，研究方向为葡萄酒酿造。E-mail：chenchengeng@hotmail.com.责任作者：张军翔（1973-），男，博士，教授，研究方向为葡萄酒酿造。E-mail：zhangjunxiang@126.com.基金项目：国家自然科学基金地区科学基金资助项目(31260392）。