ZHANG Ying,CHEN Guangcai,LIU Hong,et al.Influence of CuO Nanoparticles on Photosynthesis，Chlorophyll Fluorescence Characteristics and Antioxidant Enzyme Activities of Willow[J].Northern Horticulture,2017,41(11):83-89.[doi:10.11937/bfyy.201711018]
氧化铜纳米颗粒对柳树光合作用、叶绿素荧光和抗氧化酶活性的影响
- Title:
- Influence of CuO Nanoparticles on Photosynthesis，Chlorophyll Fluorescence Characteristics and Antioxidant Enzyme Activities of Willow
- Keywords:
- willow(Salix integar); CuO nanoparticles; photosynthesis; chlorophyll fluorescence; antioxidant enzyme
- 文献标志码:
- A
- 摘要:
- 以杞柳品种“一支笔”为试材，以Cu2+和CuO微米颗粒为对照，采用溶液培养的方法，研究了不同浓度氧化铜纳米颗粒对柳树的光合作用、叶绿素荧光参数和抗氧化酶系统的影响，以期为深入揭示CuO纳米颗粒对植物毒性效应的机理提供参考依据。结果表明：100 mg?L-1 CuO纳米颗粒和微米颗粒处理下，柳树叶片的叶绿素含量显著增加，分别增加58.9%和27.4%，Cu2+则没有显著影响。100 mg?L-1 CuO纳米颗粒处理下，柳树叶片的净光合速率(Pn）、胞间CO2浓度(Ci)及蒸腾速率(Tr)值分别减少41.2%、34.5%和51.6%，100 mg?L-1 CuO微米颗粒处理下只减少9.9%、17.2%和45.8%，而Cu2+处理下分别减少14.3%、14.1%和37.4%。Cu2+处理下PSII电子传递量子产率(ΦPSII)与对照相比没有明显变化，100 mg?L-1 CuO微米颗粒和纳米颗粒处理下ФPSII值明显降低，分别减少21.1%和23.4%。Cu2+、100 mg?L-1 CuO微米颗粒以及纳米颗粒处理下柳树叶片的超氧化物歧化酶(SOD)活性明显增加，分别增加了27.7%、28.3%和11.2%；在Cu2+处理下柳树叶片的过氧化氢酶(CAT)活性和过氧化物酶(POD)活性都显著增加，分别增加了32.2%和24.1%；100 mg?L-1 CuO微米颗粒和纳米颗粒处理下CAT活性和POD活性呈下降趋势，100 mg?L-1 CuO微米颗粒处理下CAT活性和POD活性分别减少42.6%和23.0%；100 mg?L-1 CuO纳米颗粒处理下分别减少1.84%和28.0%。该结果表明，同等离子浓度处理下，100 mg?L-1 CuO纳米颗粒对柳树叶片光合速率、PSII实际光化学量子产量等毒性大于1.5 mg?L-1 Cu2+；但CuO微米颗粒和CuO纳米颗粒同等颗粒浓度(100 mg?L-1)不同粒径颗粒处理对柳树叶片的光合作用、叶绿素荧光等的影响关系复杂，还需要进一步研究。
- Abstract:
- Salix integar ‘Yizhibi’ was used as material,Cu2+ and CuO microparticles (100 mg?L-1) were used as control.The effects of different concentrations of copper oxide nanoparticles on photosynthesis，chlorophyll fluorescence parameters and antioxidant enzyme system of Salix integra ‘Yizhibi’ were studied in order to provide a theoretical basis for revealing the mechanism of CuO nanoparticles on plant toxicity.The results showed that chlorophyll content of willow leaves increased significantly by 58.9% and 27.4%，respectively，under 100 mg?L-1 CuO nanoparticles and bulk particles，while Cu2+ were not significantly affected.The net photosynthetic rate(Pn),intercellular CO2 concentration (Ci) and transpiration rate (Tr) of the leaves under 100 mg?L-1 CuO bulk particles were decreased by 41.2%，34.5%，51.6%，respectively.And treated with 100 mg?L-1 CuO microparticles these were decreased by 9.9%，17.2% and 45.8%，respectively.While these were decreased by 14.3%，14.1% and 37.4%，respectively，under the Cu2+ treatment.The ФPSII under the treatments of Cu2+ did not change significantly compared with the control.The ФPSII treated with 100 mg?L-1 CuO microparticles and nanoparticles was significantly decreased by 21.1% and 23.4%.With the treatment of Cu2+，100 mg?L-1 CuO nanoparticles and microparticles，the SOD activity of willow leaves increased by 27.7%，28.3% and 11.20%，respectively.The CAT and POD activity increased significantly by 32.2% and 24.1%，respectively，under Cu2+ treatment.CAT and POD activity of 100 mg?L-1 CuO microparticles and nanoparticles showed a decreasing trend.CAT and POD activity decreased by 42.6% and 23.0% under treatment of 100 mg?L-1 CuO microparticles and 1.84% and 28.0% under treatment of 100 mg?L-1 CuO nanoparticles，respectively.The results showed that the toxicity of 100 mg?L-1 CuO nanoparticles to the photosynthetic rate and the photochemical yield of PSII of willow leaves were higher than 1.5 mg?L-1 Cu2+;the effects of CuO microparticles and CuO nanoparticles different particle size on the photosynthesis and chlorophyll fluorescence of willow leaves were complex and needed further study.
参考文献/References:
[1]ATHA D H,WANG H,PETERSEN E J,et al.Copper oxide nanoparticle mediated DNA damage in terrestrial plant models[J].Environmental Science & Technology,2012,46(3):1819-1827. [2]COLMAN B P,ESPINASSE B,RICHARDSON C J,et al.Emerging contaminant or an old toxin in disguise? Silver nanoparticle impacts on ecosystems[J].Environmental Science & Technology,2014,48(9):5229-5236. [3]DIMKPA C O,MCLEAN J E,LATTA D E,et al.CuO and ZnO nanoparticles：Phytotoxicity，metal speciation，and induction of oxidative stress in sand-grown wheat[J].Journal of Nanoparticle Research,2012,14(9):1-15. [4]WANG J,KOO Y,ALEXANDER A,et al.Phytostimulation of poplars and arabidopsis exposed to silver nanoparticles and Ag+ at sublethal concentrations[J].Environmental Science & Technology,2013,47(10):5442-5449. [5]SEEGER E M,BAUN A,KASTNER M,et al.Insignificant acute toxicity of TiO2 nanoparticles to willow trees[J].Journal of Soils and Sediments,2009,9(1):46-53. [6]ZHAI G,WALTERS K S,PEATE D W,et al.Transport of gold nanoparticles through plasmodesmata and precipitation of gold ions in woody poplar[J].Environmental Science & Technology Letters,2014,1(2):146-151. [7]TRAPP S.Can global biomass influence global chemical cycles?[J].Stochastic Environmental Research & Risk Assessment,2003,17(4):235-237. [8]封文强.CuO纳米颗粒对玉米的毒性效应及其在玉米体内的吸收和转运[D].杭州:中国海洋大学,2011. [9]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000. [10]CAMBROLLE J,MANCILLALEYTON J M,MUNOZVALLES S,et al.Effects of copper sulfate on growth and physiological responses of Limoniastrum monopetalum[J].Environmental Science & Pollution Research,2013,20(12):8839-8847. [11]李合生.现代植物生理学[M].2版.北京:高等教育出版社,2006. [12]STAMPOULIS D,SINHA S K,WHITE J C.Assay-dependent phytotoxicity of nanoparticles to plants[J].Environmental Science & Technology,2009,43(24):9473-9479. [13]CHEN G,MA C,MUKHERJEE A,et al.Tannic acid alleviates bulk and nanoparticle Nd2O3 toxicity in pumpkin: A physiological and molecular response[J].Nanotoxicology,2016,10(9):134. [14]赵长星,马东辉,王月福,等.施氮量和花后土壤含水量对小麦旗叶衰老及粒重的影响[J].应用生态学报,2008,19(11)：2388-2393. [15]MEDIAVILLA S,SANTIAGO H,ESCUDERO A.Stomatal and mesophyll limitations to photosynthesis in one evergreen and one deciduous mediterranean oak species[J].Photosynthetica,2002,40(4):553-559. [16]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学报,1999,16(4):444-448. [17]曹玲,王庆成,崔东海.土壤镉污染对四种阔叶树苗木叶绿素荧光特性和生长的影响[J].应用生态学报,2006,17(5):769-772. [18]付春霞,张元珍,王衍安,等.缺锌胁迫对苹果叶片光合速率及叶绿素荧光特性的影响[J].中国农业科学,2013,46(18):3826-3833. [19]吴雪霞,杨晓春,朱宗文,等.外源6-BA对低温胁迫下茄子幼苗光合作用、叶绿素荧光参数及光能分配的影响[J].植物生理学报,2013(11):1181-1188. [20]裴斌,张光灿,张淑勇,等.土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J].生态学报,2013,33(5):1386-1396. [21]毕毓芳,王安可,翟志忠,等.CCCSNs施入对毛竹叶片光合及生理特性的影响[J].安徽农业大学学报,2015,42(5):743-748.
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备注/Memo
第一作者简介：张莹（1990-），女，硕士研究生，研究方向为农业环境保护。E-mail：1072239700@qq.com.责任作者：刘泓（1968-），女，博士，教授，现主要从事植物环境毒理与环境污染评价及修复和植物营养的教学与科研等工作。E-mail：fjauliuhong@163.com.基金项目：国家自然科学基金资助项目（31470619）；中央级公益性科研院所基金资助项目（RISF2013001，RISFZ-2016-17）。