ZHANG Yongfu,NIU Yanfen,ZHAO Feng,et al.Effect of Three Biological Stains on Plant Growth and Membrane Lipid Peroxidation System of Chlorophytum comosum var.variegatum[J].Northern Horticulture,2017,41(11):75-82.[doi:10.11937/bfyy.201711017]
三种生物染色剂对银边吊兰植株生长及膜脂过氧化系统的影响
- Title:
- Effect of Three Biological Stains on Plant Growth and Membrane Lipid Peroxidation System of Chlorophytum comosum var.variegatum
- Keywords:
- Chlorophytum comosum var. variegatum; biological stain; plant growth; membrane lipid peroxidation; biomass
- 文献标志码:
- A
- 摘要:
- 以银边吊兰为试材，研究了不同浓度（0、0.5、1.0、2.0 mmol?L-1）的3种生物染色剂（甲基橙、甲基紫、中性红）对其植株生长及膜脂过氧化系统的影响。结果表明：经3个浓度的甲基紫及0.5 mmol?L-1的甲基橙和中性红处理后，银边吊兰的生物量、根系长度、叶片长度和叶片宽度均显著大于对照（CK），2.0 mmol?L-1处理则使以上形态指标显著小于CK，而1.0 mmol?L-1处理后则与CK差异不显著。处理后，叶片丙二醛、过氧化氢含量、氧自由基产生速率及过氧化物酶（POD）活性在整个试验期间均高于CK，且随着浓度的增加和时间的延长有明显的上升趋势；叶片超氧化物歧化酶（SOD）活性随着时间的延长有先上升后下降的趋势，但2.0 mmol?L-1处理的活性低于低浓度处理的活性，表明此时抗氧化系统已开始紊乱。综上，结合染色剂对植株生长和膜脂过氧化系统的影响来看，1.0 mmol?L-1的甲基橙、甲基紫和中性红比较适合银边吊兰的活体染色。
- Abstract:
- Chlorophytum comosum var. variegatum was used as test material，the effect of three biological stains (methyl orange，methyl violet and neutral red) of different concentration (0,0.5,1.0,2.0 mmol?L-1) on plant growth and membrane lipid peroxidation system were studied.The results showed that after treatment with the three concentrations of methyl violet and 0.5 mmol?L-1 of methyl orange and neutral red，biomass，root length，leaf length and leaf width of Chlorophytum comosum var. variegatum were significantly larger than those of control (CK)，after 2.0 mmol?L-1 treatment，the above morphological indicators were significantly less than CK，whereas after 1.0 mmol?L-1 treatment and CK were not significantly different.After treatment，MDA，H2O2 content，oxygen free radical production rate and peroxidase (POD) activity of leaves were higher than CK during the whole experiment，and with the increase of concentration and time，there was an obvious upward trend；the superoxide dismutase (SOD) increased first and then decreased with time，but the activity of 2.0 mmol?L-1 treatment was lower than that of low concentration，which indicated that the antioxidant system was beginning to disorder.Thus，combined with the effect of dying agent on plant growth and membrane lipid peroxidation system，1.0 mmol?L-1 methyl orange，methyl violet and neutral red were more suitable for Chlorophytum comosum var. variegatum intravital staining.
参考文献/References:
[1]余亚白,陈源,赖呈纯,等.室内空气净化植物的研究与利用现状及应用前景[J].福建农业学报,2006,21(4):425-429. [2]韩科厅,胡可,戴思兰.观赏植物花色的分子设计[J].分子植物育种,2008,6(1):16-24. [3]韩智豪,朱艳,鲁婷,等.常用染色剂对水仙的染色效应[J].农业工程技术,2013(5):58-60. [4]李宁义,韩艳茹,常秀丽,等.月季鲜切花染色技术的研究[J].温室园艺,2005(10):24-25. [5]杨志明.马蹄莲鲜切花染色技术的研究[J].北方园艺,2006(5):120-121. [6]章玉平,刘武,朱运美,等.7种食用色素对香石竹的染色效应[J].河北农业科学,2008,12(5):26-27,29. [7]刘立波,岳君,窦海洋,等.菊花鲜切花染色技术的研究[J].吉林农业大学学报,2004,26(6):642-643,648. [8]王华,耿进华.百合切花染色技术的研究[J].安徽农学通报,2007,13(5):66-66,15. [9]章玉平.食用色素对满天星切花的染色效应[J].安徽农学通报,2008,14(15):136-137,54. [10]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000. [11]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000. [12]刘立波,岳君,窦海洋,等.菊花鲜切花染色技术的研究[J].吉林农业大学学报,2004,26(6):642-643,648. [13]秀妹,刘信宝,李志华,等.不同水分胁迫下水杨酸对分枝期扁蓿豆生长及光合生理的影响[J].草业学报,2012,21(6):82-93. [14]樊瑞苹,周琴,周波,等.盐胁迫对高羊茅生长及抗氧化系统的影响[J].草业学报,2012,21(1):112-117. [15]张永福,任禛,陈泽斌,等.水杨酸对缓解葡萄苗铝毒害的生理机制[J].华北农学报,2015,30(1):182-187. [16]贾学静,董立花,丁春邦,等.干旱胁迫对金心吊兰叶片活性氧及其清除系统的影响[J].草业学报,2013,22(5)：248-255. [17]付畅,关砀,徐娜.盐胁迫对野生和栽培大豆中抗氧化酶活性的影响[J].大豆科学,2007,26(2):144-148. [18]GAJEWSKA E,SKLODOWSKA M.Effect of nickel on ROS content and antioxidative enzyme activities in wheat leaves[J].Bio Metals,2007,20:27-36. [19]欧晓明,雷满香,王晓光,等.新杀虫剂HNPC-A9908对蛋白核小球藻生理生化特性的影响[J].农业环境科学学报,2004,23(1):154-158. [20]林琪,侯立自,韩伟,等.干旱胁迫对小麦旗叶活性氧代谢及灌浆速率的影响[J].西北植物学报,2004,23(12):2152-2156. [21]华春,周泉澄,张边江,等.毕氏海蓬子和盐角草幼苗对PEG-6000模拟干旱的生理响应[J].干旱区研究,2009,26(5):702-707.
备注/Memo
第一作者简介：张永福（1981-），男，博士，副教授，研究方向为园艺植物抗性生理。E-mail：123017360@qq.com.基金项目：国家自然科学基金资助项目（31660559）；云南省高校优势特色重点学科（生态学）建设资助项目。