[1]刘卫锋,辛明,周秀艳,等.黄瓜CsPAP-fib基因的克隆与序列分析[J].北方园艺,2017,41(20):40-45.[doi:10.11937/bfyy.20171060]
　LIU Weifeng,XIN Ming,ZHOU Xiuyan,et al.Cloning and Sequence Analysis of CsPAP-fib Gene in Cucumber[J].Northern Horticulture,2017,41(20):40-45.[doi:10.11937/bfyy.20171060]
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黄瓜CsPAP-fib基因的克隆与序列分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年20 页码: 40-45 栏目: 出版日期: 2017-10-30
Title:
Cloning and Sequence Analysis of CsPAP-fib Gene in Cucumber
作者:
刘卫锋; 辛明; 周秀艳; 秦智伟
东北农业大学 园艺园林学院，黑龙江 哈尔滨 150030
Author(s):
LIU Weifeng; XIN Ming; ZHOU Xiuyan; QIN Zhiwei
College of Horticulture and Architecture Landscape,Northeast Agricultural University,Harbin,Heilongjiang 150030
关键词:
黄瓜; CsPAP-fib基因; 基因克隆; 序列分析
Keywords:
cucumber; CsPAP-fib gene; clone; sequence analysis
DOI:
10.11937/bfyy.20171060
文献标志码:
A
摘要:
以温敏型黄瓜‘C09-123’为试材，通过对不同夜温处理的黄瓜幼叶进行差异蛋白质鉴定，筛选出一个与低夜温影响黄瓜雌性形成密切相关的蛋白，采用生物信息学方法，对该蛋白进行生物信息学分析,并运用基因克隆技术克隆了该基因，以期为明确该基因在黄瓜性别分化中的作用机制奠定基础，也为进一步试验研究提供基因材料。结果表明：该基因全长870 bp，编码289个氨基酸，该基因编码的蛋白质属于叶绿体中一个不稳定的疏水蛋白质，无明显信号肽，无跨膜结构。与甜瓜为同一进化分支，同源性较高。该蛋白属于PAP-fibrillin蛋白家族，因此将编码该蛋白的基因命名为CsPAP-fib。
Abstract:
The tempetature-sensitive cucumber cultivar ‘C09-123’ was used as material in this study,a protein which concerned the formation of cucumber femaleness under low night temperature had been found by the identification of differential proteins in cucumber young leaves treated by different night temperatures,then was analysed by bioinformatics technology.The gene encoded this protein was cloned by cloning technique to establish a foundation for confirming the functional mechanisms of CsPAP-fib in cucumber sex differentiation,and provide the genetic material for further research.The results showed that the gene length was 870 bp,encoding 289 amino acids.The protein encoded by this gene was an unstable,hydrophobic and non-transmembrane protein without signal peptide located in chloroplast,and it was highly homologous to Cucumis melo L.The gene was named as CsPAP-fib,based on the protein family PAP-fibrillin which the protein encoded by the gene.

参考文献/References:

[1]张微,李锡香.黄瓜花芽性别分化的研究进展与展望[J].华北农学报,2015,30(s1):74-80.

[2]MALEPSZY S,NIEMIROWICZ-SZCZYTT K.Sex determination in cucumber (Cucumis sativus ) as a model system for molecular biology[J].Plant Science,1991,80(1):39-47.
[3]TANURDZIC M,BANKS J A.Sex-determining mechanisms in land plants[J].Plant Cell,2004,16:S61-S71.
[4]闫晨曦.影响植物新陈代谢的环境因素分析[J].现代园艺,2011(12x):4.
[5]汪俏梅,曾广文.苦瓜性别表现研究概况及展望[J].中国蔬菜,1995(4):50-53.
[6]马刘峰,易海艳,易霞,等.不同光周期和温度处理对黄瓜花芽分化的影响[J].北方园艺,2012(21):25-26.
[7]程国辉,秦智伟,冯卓,等.光周期和温度对黄瓜品种‘C09-123’性别分化的影响[J].北方园艺,2012(15):35-37.
[8]厉建梅,秦智伟,周秀艳,等.黄瓜雌性性状的遗传分析[J].中国农业科学,2011,44(15):3169-3176.
[9]刘欣童.低夜温下黄瓜雌性分化相关代谢与蛋白质变化的初步分析[D].哈尔滨:东北农业大学,2016.
[10]PRUVOT G,CUIN S,PELTIER G,et al.Characterization of a novel drought-induced 34-kDa protein located in the thylakoids of Solanum tuberosum L.plants[J].Planta,1996,198(3):471-479.
[11]滕蕾.Fibrillin转入甘蓝型油菜中的研究[D].雅安：四川大学,2006.
[12]CHEN R,TIAN M,WU X,et al.Differential global gene expression changes in response to low nitrogen stress in two maize inbred lines with contrasting low nitrogen tolerance[J].Genes & Genomics,2011,33(5):491.
[13]周慧梅,谢德颖,邹娟子,等.植物质体Fibrillin蛋白家族的起源、结构和功能[J].中国农业科技导报,2014,16(4):41-49.
[14]WUTTKE H G.Chromoplasts in Rosa rugosa：Development and chemical characterization of tubular elements[J].Zeitschrift Für Naturforschung C,1976,31(7-8):456-460.
[15]WINKENBACH F,FALK H,LIEDVOGEL B,et al.Chromoplasts of Tropaeolum majus L.：Isolation and characterization of lipoprotein elements[J].Planta,1976,128(1):23-28.
[16]KNOTH R,HANSMANN P,SITTE P.Chromoplasts of Palisota barteri,and the molecular structure of chromoplast tubules.[J].Planta,1986,168(2):167-174.
[17]EMTER O,FALK H,SITTE P.Specific carotenoids and proteins as prerequisites for chromoplast tubule formation[J].Protoplasma,1990,157(1-3):128-135.
[18]VISHNEVETSKY M,OVADIS M,ITZHAKI H,et al.Molecular cloning of a carotenoid-associated protein from Cucumis sativus corollas：Homologous genes involved in carotenoid sequestration in chromoplasts[J].Plant Journal for Cell & Molecular Biology,1996,10(6):1111-1118.
[19]HERNNDEZPINZN I,ROSS J H,BARNES K A,et al.Composition and role of tapetal lipid bodies in the biogenesis of the pollen coat of Brassica napus[J].Planta,1999,208(4):588-598.
[20]KESSLER F,SCHNELL D,BLOBEL G.Identification of proteins associated with plastoglobules isolated from pea (Pisum sativum L.) chloroplasts[J].Planta,1999,208(1):107-113.
[21]VIDI P A,KANWISCHER M,BAGINSKY S,et al.Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles[J].Journal of Biological Chemistry,2006,281(16):11225-11234.
[22]SINGH D K,MCNELLIS T W.Fibrillin protein function：The tip of the iceberg?〖KG-*2〗[J].Trends in Plant Science,2011,16(8):432-441.
[23]GILLET B,BEYLY A,PELTIER G,et al.Molecular characterization of CDSP 34,a chloroplastic protein induced by water deficit in Solanum tuberosum L.plants,and regulation of CDSP 34 expression by ABA and high illumination[J].Plant Journal,1998,16(2):257-262.
[24]邹晓艳.黄瓜性型遗传规律及性别决定相关基因的分布和表达研究[D].北京：中国农业科学院,2007.
[25]YAMASAKI S,FUJII N,TAKAHASHI H.Characterization of ethylene effects on sex determination in cucumber plants[J].Sexual Plant Reproduction,2003,16(3):103-111.
[26]梁永宏,李广林,郭韬,等.黄瓜性型分化的分子机制[J].生命科学,2010(11):1177-1183.

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备注/Memo

第一作者简介：刘卫锋(1990-），男，硕士研究生，研究方向为黄瓜分子遗传育种。E-mail：liuweifeng3385@126.com.

责任作者：秦智伟(1957-），男，博士，教授，博士生导师，研究方向为黄瓜遗传育种。E-mail：qzw303@126.com.
基金项目：黑龙江省普通本科高等学校青年创新人才培养计划资助项目(UNPYSCT-2016007）。
收稿日期：2017-07-10

更新日期/Last Update: 2017-10-31