LI Hanfen,XU Ying,SU Peng,et al.Effect of Stone Cell Development on Hard-end Disorder of ‘Whangkumbae’[J].Northern Horticulture,2017,41(10):20-24.[doi:10.11937/bfyy.201710005]
石细胞发育对“黄金梨”果肉硬化症的影响
- Title:
- Effect of Stone Cell Development on Hard-end Disorder of ‘Whangkumbae’
- 文献标志码:
- A
- 摘要:
- 选用“黄金梨”发病果实与正常果为试材，通过对病果与正常果单果质量、果形指数、色差、石细胞含量、果胶甲脂酶（PME）活性、苯丙氨酸解氨酶（PLA）活性等指标的测定，探索“黄金梨”果肉硬化的发病原因。结果表明：病果石细胞含量明显高于正常果，病果苯丙氨酸解氨酶活性显著高于正常果，但病果与正常果相比较，在果胶甲酯酶活性上无明显差异；说明“黄金梨”果肉硬化与石细胞的发育和苯丙氨酸解氨酶关系密切，而果胶甲酯酶活性对果肉硬化无明显影响。
- Abstract:
- For exploring the causes of the hard-end disorder of ‘Whangkeumbae’ fruits，the fruits with hard-end disease were selected as test materials in the experiment，via the determination of fruit weight，fruit shape index，the color difference，the content of stone cell，pectinesterase (PME)，phenylalanine ammonia-lyases (PLA) of disease fruit and normal fruit，to discuss the reason causing to the hard-end disorder of ‘Whangkeumbae’.The results showed that stone cell content in diseased fruit was significantly higher than normal fruit，the PLA activity in diseased fruit was significantly higher than normal fruit，but there was no difference on the PME activity between diseased fruit and normal fruit.The above results pointed out that PLA was closed to the hard-end disorder of Whangkeumbae.However，PME activity had no obvious effect on the pulp hardening.
参考文献/References:
[1]孟学理,松增琦.黄金梨:一个优良的果树品种[J].生物学通报,2003(6):61. [2]YAMAMOTO T，WATANABE S.Initial time of development of hard end disorder in‘Bartlett’pear[J].J Japan Soc Hort Sci,1982,51(2):142-151. [3]马春晖,李鼎立,王然.黄金梨“铁头病”果肉组织切片观察[J].北方园艺,2014(13):109-113. [4]林河通,席芳,陈绍军.黄花梨果实采后软化生理基础[J].中国农业科学,2003,36(3):349-352. [5]齐秀东,魏建梅,高海生,等.梨果实发育软化与果胶多糖降解特性的关系[J].中国农业科学,2015,48(15):3027-3037. [6]耿飒,徐存拴,李玉昌.木质素的生物合成及其调控研究进展[J].西北植物学报,2003,23(1):171-181. [7]陶书田,张绍铃,乔勇进,等.梨果实发育过程中石细胞团及几种相关酶活性变化的研究[J].果树学报,2004(6):516-520. [8]蒋明凤.日本梨成熟过程中细胞壁组分变化及石细胞的形态学观察[D].哈尔滨:东北农业大学,2013. [9]刘玲,李疆,覃伟铭.库尔勒香梨果实石细胞发育及其对果实肉质影响初探[J].西北植物学报,2005,25(10):1965-1968. [10]王玲利,刘超,黄艳花,等.‘黄冠’梨采后热处理和钙处理对其钙形态及细胞壁物质代谢的影响[J].园艺学报,2014,41(2):249-258. [11]魏建梅,马锋旺,关军锋,等.京白梨果实后熟软化过程中细胞壁代谢及其调控[J].中国农业科学,2009,42(8):2987-2996. [12]刘剑锋,程云清,彭抒昂.梨采后细胞壁成分及果胶酶活性与果肉质地的关系[J].园艺学报,2004,31(5):579-583. [13]聂继云,李静,杨振锋,等.冷冻法测定梨的石细胞含量[J].果树学报,2006,23(1):133-135. [14]KOUKOL J，CORM E E.The metabolism of aromatic compounds in high plant.IV.Purification and properties of the phenylalanine deaminase of Herdeum vulagare[J].Journal of Biology and Chemistry,1961,236：2692-2698. [15]WEI J M，MA F W，SHI S G，et al.Changes and the postharvest regulation in the activity and gene expression of enzymes related to cell wall degradation in ripening apple fruit[J].Postharvest Biology and Technology，2010,56:147-154. [16]于娟娟.砀山酥梨石细胞发育过程中POD酶学特性及POD克隆[D].合肥:安徽农业大学,2011. [17]刘艳,鲁晓燕,黄学东,等.梨石细胞含量与PAL活性和果实硬度的相关性研究[J].新疆农业科学,2011,48(9):1579-1601.
相似文献/References:
[1]王宝刚,李文生,冯晓元,等.1-MCP对冷藏“黄金梨”果实品质与生理变化的影响[J].北方园艺,2012,36(09):165.
WANG Bao-gang,LI Wen-sheng,FENG Xiao-yuan,et al.Effects of 1-MCP Treatment on Quality and Physiology of ‘Whangkeumbae’ Pear During Cold Storage[J].Northern Horticulture,2012,36(10):165.
备注/Memo
第一作者简介：李含芬（1970-），女，甘肃白银人，本科，馆员，现主要从事图书管理及园艺学等研究工作。E-mail：lihanfen1@sina.com.责任作者：马春晖(1966-)，男，博士，副教授，现主要从事果树栽培学等研究工作。E-mail：machunhui2000@163.com.基金项目：国家现代农业（梨）产业技术体系建设资助项目（Nycytx-29-06）；山东省自然科学基金资助项目（ZR2014CL026）；山东省重点研发资助项目（2015GNC110022）。