[1]王锋尖,周向宇,甘露.一株野生食用菌的形态及分子鉴定[J].北方园艺,2017,41(21):159-162.[doi:10.11937/bfyy.20170834]
　WANG Fengjian,ZHOU Xiangyu,GAN Lu.Identification of a Wild Mushroom Based on Its Morphology and ITS Sequence[J].Northern Horticulture,2017,41(21):159-162.[doi:10.11937/bfyy.20170834]
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一株野生食用菌的形态及分子鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年21 页码: 159-162 栏目: 出版日期: 2017-11-15
Title:
Identification of a Wild Mushroom Based on Its Morphology and ITS Sequence
作者:
王锋尖; 周向宇; 甘露
汉江师范学院 生物化学与环境工程系，湖北 十堰 442000
Author(s):
WANG Fengjian; ZHOU Xiangyu; GAN Lu
Department of Biochemistry and Environment Engineering,Hanjiang Normal University,Shiyan,Hubei 442000
关键词:
野生菌; ITS 序列; 形态学鉴定; 湖北新记录种; 赛武当自然保护区
Keywords:
wild mushroom; ITS sequence; identification of morphology; newly recorded species of Hubei; Saiwudang Nature Reserve
DOI:
10.11937/bfyy.20170834
文献标志码:
A
摘要:
以采自湖北赛武当自然保护区内的一株野生食用菌的子实体为试材，研究了其生态环境、形态结构，并从形态学角度对其进行了鉴定；对其ITS序列进行了扩增、测序，并在基因组数据库中比对确定其亲缘关系。结果表明：该野生菌为金盖褐环柄菇（Phaeolepiota aurea），属湖北省新记录种。该研究结果为进一步开发其应用价值奠定了基础。
Abstract:
The ecological environment and the morphological structure of a wild mushroom picked from Saiwudang Nature Reserve were studied in this experiment.Meanwhile,the species was identified based on its morphological characteristics. Molecular identification was done by extracting its DNA,amplifying and sequencing its ITS sequence,and comparing the result with GenBank to determine the genetic relationship.The results showed that the wild strain was identified finally as the Phaeolepiota aurea, a new record species in Hubei Province.The result laid a foundation for the further development of its application value.

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备注/Memo

第一作者简介：王锋尖(1976-)，男，硕士，副教授，研究方向为野生食用菌资源调查与多样性。E-mail：wnfnjn@126.com.

基金项目：湖北省教育厅科学技术研究资助项目（B2017220）;汉江师范学院重点科研资助项目（2012A07）。
收稿日期：2017-04-06

更新日期/Last Update: 2017-11-15