[1]宋启玲,王贵元,孟想想,等.不同消毒方式及激素配比对毛桃愈伤组织形成的影响[J].北方园艺,2017,41(17):61-66.[doi:10.11937/bfyy.20170803]
　SONG Qiling,WANG Guiyuan,MENG Xiangxiang,et al.Effect of Different Disinfection Methods and Hormone Ratio on Callus Formation of Prunus persica (L.) Batsch[J].Northern Horticulture,2017,41(17):61-66.[doi:10.11937/bfyy.20170803]
点击复制

不同消毒方式及激素配比对毛桃愈伤组织形成的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年17 页码: 61-66 栏目: 出版日期: 2017-09-15
Title:
Effect of Different Disinfection Methods and Hormone Ratio on Callus Formation of Prunus persica (L.) Batsch
作者:
宋启玲; 王贵元; 孟想想; 叶家保; 张威威; 许锋
长江大学 园艺园林学院，湖北 荆州 434025
Author(s):
SONG Qiling; WANG Guiyuan; MENG Xiangxiang; YE Jiabao; ZHANG Weiwei; XU Feng
College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025
关键词:
毛桃; 茎段培养; 叶片培养; 消毒方法; 激素配比
Keywords:
Prunus persica(L.) Batsch; culture of stem segment; culture of tender leaves; disinfection methods; types and concentrations of hormones
DOI:
10.11937/bfyy.20170803
文献标志码:
A
摘要:
以毛桃品种“春美”当年生枝的茎段以及嫩叶为外植体，采用单因子试验方法，研究了在不同消毒方法下接种的2种外植体的污染率和愈伤组织形成率，以及不同激素种类和浓度配比下2种外植体的愈伤组织形成率，以期为建立毛桃再生体系以及毛桃的遗传转化提供参考依据。结果表明：毛桃茎段的表面消毒以75%酒精30 s+2.5% NaClO 10 min为佳；适合茎段的最佳初代培养基为MS+6-BA 1.0 mg?L-1 + IBA 0.5 mg?L-1+GA3 0.5 mg?L-1，毛桃叶片的表面消毒以75%酒精30 s+2.5% NaClO 6 min+0.5% NaClO 10 min最佳，适合叶片的最佳初代培养基为1/2MS+TDZ 1.5 mg?L-1+NAA 0.1 mg?L-1。
Abstract:
The stem segments and tender leaves which were current-year branches of Prunus persica (L.) Batsch were used as explants.Using the single factor design,the effects of different disinfection methods on the inoculated of two explants contamination rate and callus formation rate,and the rate of callus formation under different hormone types and concentration ratio were studied,in order to lay a foundation for the regeneration system and genetic transformation of P.persica.The results showed that the optimal surface disinfection of the stem segments of P.persica was 75% alcohol 30 seconds+2.5% NaClO 10 minutes；the optimum medium for stem segments was MS + 6-BA 1.0 mg?L-1+IBA 0.5 mg?L-1+GA3 0.5 mg ?L-1.And the optimal surface disinfection of the tender leaves of P.persica was 75% alcohol 30 seconds+2.5% NaClO 6 minutes+0.5% NaClO 10 minutes；the optimum medium for tender leaves was 1/2MS+TDZ 1.5 mg?L-1+NAA 0.1 mg?L-1.In this study,the regeneration system from stem segments and leaves of P.persica was established,which laid the foundation for the genetic transformation of peach.

参考文献/References:

[1]龙兴桂.现代中国果树栽培(落叶果卷)[M].北京:中国林业出版社,2000:235.

[2]DEBEAUJON I,LONKLOOSTERZIEL K M,KOORNNEEF M.Influence of the testa on seed dormancy,germination,and longevity in Arabidopsis[J].Plant Physiology,2000,122(2)：403-414.
[3]柴春燕,胡东旭.宜兴百合特性及高产栽培技术[J].广西园艺，2003(3):24-25.
[4]周平,郭瑞,廖汝玉,等.2011-2013年度国外桃遗传育种与生产研究进展[J].中国南方果树,2014,43(4):34-37.
[5]罗克明,万明长,罗孝明，等.毛桃砧嫁接桃、杏、李的亲和力研究[J].贵州农业科学，2009，37(5):20-21.
[6]叶航，简恒，朱立新，等.4种桃砧木对南方根结线虫抗性研究[J].中国果树，2006(4):39-42.
[7]王雯君,贾克功,朱立新,等.毛桃对北方根结线虫的抗性研究[J].中国农业大学学报,2009,14(4):71-76.
[8]许建兰,马瑞娟,俞明亮,等.‘银河’蟠桃果实性状遗传评价及育种利用探讨[J].果树学报,2014(5):769-775.
[9]闫承璞.桃离体培养及叶片再生体系的建立[D].北京：中国农业科学院，2006.
[10]MOK M C,MOK D W S,TURNER J E,et al.Biological and biochemical effects of cytokinin-active phenylurea derivatives in tissue culture systems[J].Hort Science,1987（22）：1194-1197.
[11]王旭炜，付杰，王青，等.TDZ在木本植物再生中的应用趋势及桑树中的前景[J].蚕学通讯，2014(1):22-30.
[12]徐晓峰，黄学林.TDZ:一种有效的植物生长调节剂[J].植物学报，2003，20(2):227-237.
[13]HUTCHINSON M J,SAXENA P K.Acetylsalicylic acid enhances and synchronizes thidiazuron-induced somatic embryogenesis in geranium (Pelargonium×Hortorum Bailey) tissue cultures[J].Plant Cell Reports,1996,15(7)：512-515.
[14]VISSER C,QURESHI J A,GILL R,et al.Morphoregulatory role of thidiazuron：Substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures[J].Plant Physiology,1992,99(4)：1704-1707.
[15]DECLERCK V,KORBAN S S.Influence of growth regulators and carbon sources on callus induction,growth and morphogenesis from leaf tissues of peach (Prunus persica (L).Batsch)[J].Journal of Horticultural Science,1996,71(1)：49-55.
[16]刘燕,龙成昌,巫华美,等.贵州金丝桃组培快速繁育技术研究[J].种子,2011,30(10):40-42.
[17]ZHOU H C,MING L,XIA Z,et al.Plant regeneration from in vitro leaves of the peach rootstock ‘Nemaguard’ (Prunus persica×P.davidiana)[J].Plant Cell,Tissue and Organ Culture,2010,101(1)：79-87.
[18]郭伟伟，孟庆杰，黄勇，等.‘黄金冠’桃组织培养的初步研究[J].北方园艺，2010(19):142-144.
[19]孙俊，孙其宝，俞飞飞.桃快速繁殖技术体系的研究[J].安徽农业科学，2003，31(5):731-732.
[20]任凝辉，武荣花，王献，等.矮生一品红苞片外植体组织培养技术研究[J].河南农业大学学报，2001，35(3):239-240.
[21]STYER D J,CHIN C K.Meristem and shoot-tip culture for propagation,pathogen elimination,and germplasm preservation[J].Horticultural Reviews,2010(5)：221-227.[22]SAN B,LI Z,HU Q,et al.Adventitious shoot regeneration from in vitro cultured leaf explants of peach rootstock Guardian ,is significantly enhanced by silver thiosulfate[J].Plant Cell,Tissue and Organ Culture,2015,120(2):757-765.

相似文献/References:

[1]肖云英,孙国超,廖秋渝,等.γ-氨基丁酸对毛桃幼苗生长及镉积累的影响[J].北方园艺,2024,(19):31.[doi:10.11937/bfyy.20241330]
　XIAO Yunying,SUN Guochao,LIAO Qiuyu,et al.Effects of γ-aminobutyric Acid on Growth and Cadmium Accumulation of Peach Seedlings[J].Northern Horticulture,2024,(17):31.[doi:10.11937/bfyy.20241330]

备注/Memo

第一作者简介：宋启玲（1994-），女，硕士研究生，研究方向为植物分子生物学。E-mail：song70@126.com.

责任作者：许锋（1979-），男，博士，教授，现主要从事药用植物次生代谢等研究工作。E-mail：xufeng198@126.com.
基金项目：国家自然科学基金资助项目（31300574）。
收稿日期：2017-04-06

更新日期/Last Update: 2017-09-29