CAO Yang,HE Mingxia,GAO Feng,et al.Genetic Diversity Analysis of Phlebopus portentosus Based on SSR Markers[J].Northern Horticulture,2017,41(08):111-114.[doi:10.11937/bfyy.201708025]
基于SSR标记的暗褐网柄牛肝菌遗传多样性分析
- Title:
- Genetic Diversity Analysis of Phlebopus portentosus Based on SSR Markers
- Keywords:
- Phlebopus portentosus; SSR markers; genetic diversity
- 文献标志码:
- A
- 摘要:
- 以33份暗褐网柄牛肝菌样品为试材，采用SSR标记技术，对其遗传多样性进行研究，以期为暗褐网柄牛肝菌商业菌株鉴定奠定基础。结果表明：15个SSR标记中有5个标记在供试样品中表现出多态性，每个标记检测到2~6个等位基因，平均3.20个，Shannon′s 信息指数平均值0.76，多态性信息含量平均值0.43。聚类分析结果显示，在相似系数为0.99时，供试材料可被分为11个类群，对应11种多位点分子表型，与样品的来源有较好的对应关系。根据多位点分子表型构建了供试材料的SSR指纹图谱。
- Abstract:
- In this study,the genetic diversity of 33 Phlebopus portentosus materials was analyzed by using SSR markers in order to lay the foundation of commercial strains indentification of Phlebopus protentaosus.The results showed that 5 out of 15 SSR markers represented polymorphism in testing samples,2-6 alleles were found in each marker and the mean value was 3.20.The average values of Shannon′s information index and polymorphism information content were 0.76 and 0.43 respectively.The testing samples were clustered into 11 groups at the genetic similarity 0.99 on a UPGMA dendrogram,which corresponded to 11 multiple loci molecular phenotypes and the cloning origin of the samples.The codes of SSR fingerprinting were constructed based on these multiple loci molecular phenotypes.
参考文献/References:
[1]SHARMA P C,GROVER A,KAHL G.Mining microsatellites in eukaryotic genomes[J].Trends Biotechnol,2007,25:490-498. [2]LI Y C,KOROL A B,FAHIMA T,et al.Microsatellites within genes:structure,function,and evolution[J].Mol Biol Evol,2004,21:991-1007. [3]MURAT C,RICCIONI C,BELFIORI B,et al.Distribution of microsatellites in the Perigord black truffle genome and identification of new molecular markers[J].Fungal Genetics and Biology,2011,48:592-601. [4]JUN Q,HAIBIN X,JINGYUAN S,et al.Genome-wide analysis of simple sequence repeats in the model medicinal mushroom Ganodermalucidum[J].Gene,2013,512:331-336. [5]臧穆.我国东喜马拉雅及其邻区牛肝菌目的研究(续)[J].云南植物研究,1986,8(1):1-22. [6]臧穆.中国真菌志[M].第22卷.北京:科学出版社,2006:1-215. [7]杨祝良,臧穆.中国南部高等真菌的热带亲缘[J].云南植物研究,2003,25(2):129-144. [8]HEINEMANN P,RAMMELOO J.Observations sur le genre Phlebopus(Boletineae)[J].Mycotaxon,1982,15:384-404. [9]BARBARA P S.An annotated checklist of Agarics and Boleti recorded from New Zealand[J].New Zealand Journal of Botany,1987,25(2):185-215. [10]WATLING R.The relationships and possible distributional patterns of Boletes in South-East Asia[J].Mycological Research,2001,105(12):1440-1448. [11]BANDALA V M,MONTOYA L,JARVIO D.Two interesting records of Boletes found in coffee plantations in Eastern Mexico[J].Persoonia,2004,18(3):365-380. [12]张春霞,纪开萍,何明霞,等.暗褐网柄牛肝菌子实体营养成分分析[J].云南大学学报(自然科学版),2010,32(6):702-704. [13]JI K P,CAO Y,ZHANG C X,et al.Cultivation of Phlebopus portentosusin southern China[J].Mycological Progress,2011,10:293-300. [14]SANMEE R,LUMYONG P,DELL B,et al.In vitro cultivation and fruit body formation of the black bolete,Phlebopus portentosus,a popular edible ectomycorrhizal fungus in Thailand[J].Mycoscience,2010,51:15-22. [15]云南省热带作物科学研究所,云南大学.暗褐网柄牛肝菌SSR分子标记：CN 104946763 A[P].2015-09-30. [16]YEH F C,YANG R C,BOYLE T B J,et al.POPGENE,the user friendly shareware for population genetic analysis[M].Edmonton:Molecular Biology and Biotechnology Centre University of Alberta,1997. [17]BOTSTEIN D,WHITE R L,SKOLNICK M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American Journal of Human Genetics,1980,32(3):314-331． [18]ROHLF F J.NTSYSpc:numerical taxonomy system,ver.2.10[R].Exeter Publishing,Ltd.:Setauket,NY,2000. [19]LABB J,MURAT C,MORIN E,et al.Survey and analysis of simple sequence repeats in the Laccaria bicolor genome,with development of microsatellite markers[J].Current Genetics,2011,57(2):75-88. [20]WANG Y,CHEN M,WANG H,et al.Microsatellites in the genome of the edible mushroom,Volvariella volvacea[J].Biomed Research International,2014(1):234-240.
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备注/Memo
第一作者简介：曹旸（1983-），女，云南西双版纳人，博士，助理研究员，现主要从事食用菌学等研究工作。E-mail：caoyang8352@163.com.基金项目：国家自然科学地区基金资助项目（31560008）；云南省科研院所技术开发专项资助项目（2015DC014）；中国科学院“西部之光”人才培养资助项目（326）；景洪市科技三项费资助项目（2013-46）。