LI Fei,ZHANG Hao,LIU Liang,et al.Effects of Exogenous Ca2+ on Stomatal Traits and Gas Exchange Parameters of Anthurium scherzerianum[J].Northern Horticulture,2017,41(08):80-85.[doi:10.11937/bfyy.201708019]
外源钙离子对红掌叶片气孔特征及气体交换参数的影响
- Title:
- Effects of Exogenous Ca2+ on Stomatal Traits and Gas Exchange Parameters of Anthurium scherzerianum
- Keywords:
- Ca2+; photosynthesis; transpiration rate; stomatal conductance; spatial distribution pattern
- 文献标志码:
- A
- 摘要:
- 以红掌叶片为试材，采用不同浓度Ca(NO3)2（0、2、4、6、8 mmol?L-1）溶液对红掌进行2周的培养，对气孔密度、气孔解剖结构(气孔长度、宽度、周长、面积、〖JP2〗形状指数和面积指数)，气孔空间分布格局和叶片气体交换进行了测定，以探究外源Ca2+对红掌叶片气孔特征及气体交换参数的影响。结果表明：当Ca2+浓度为4 mmol?L-1时，气孔的周长达到最大值，且此时气孔变得最为扁长。另外，Ca2+浓度的升高使得气孔的宽度增加约10%。当Ca2+浓度为6 mmol?L-1时气孔密度最高，但气孔空间格局却最不规则。然而，该研究中设置的5个Ca2+浓度均没有对气孔开口面积产生显著的影响（P<0.05）。另外，随着外源钙离子浓度的增加红掌的净光合反应速率(Pn)呈现出升高的趋势，但各处理间Pn差异不显著，且均低于对照组。此外，红掌叶片的蒸腾速率和气孔导度在不同外源钙离子处理下也没有发生显著的变化。说明外源钙处理对于红掌气孔特征和生理过程的影响非常有限，这可能是由于外源钙提高气孔密度有利于气体交换的正效应同减弱气孔分布格局规则性不利于气体交换的负相应相抵消，从而导致红掌的生理参数没有发生显著的变化。
- Abstract:
- Leaves of Anthurium scherzerianum were used as materials.Stomatal density,stomatal anatomic structure (stomatal length，width，perimeter，area，shape index and area index)，spatial distribution pattern，and leaf gas exchange of Anthurium scherzerianum were examined with changing the concentration of Ca(NO3)2 by 0 (CK)，2，4，6，8 mmol?L-1 in the nutrition medium to study effects of exogenous Ca2+ on the stomatal traits and gas exchange parameters of Anthurium scherzerianum.The results showed that the stomatal perimeter reached its maximum and the stomata characterized the most prolate shape under the Ca2+ of 4 mmol?L-1.Meanwhile，the stomatal aperture width of 8 mmol?L-1 was significantly increased by 10% relative to that of CK.The leaves featured with the most of stomata per millimeter leaf area and the highly irregular spatial distribution pattern under the treatment of 6 mmol?L-1.However，the stomatal aperture area was not significantly changed by all the Ca2+ treatments(P<0.05).Moreover，the net photosynthetic rate(Pn) was gradually increased with the increase of Ca2+，whereas the Pn of CK was higher than those of four Ca2+ treatments，and no significant difference on Pn was found among the CK and the four Ca2+ treatments.In addition，we also found that Ca2+ barely affected the leaf leaf stomatal conductance(Gs) and transpiration rate (E) of Anthurium scherzerianum.These results suggested that Ca2+ had little impact on the stomatal traits and physiological processes might due mainly to the offset on the gas exchange from the positive effects by increasing stomatal density and the negative effects by declining the regularity of stomatal spatial distribution pattern.
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备注/Memo
第一作者简介：李菲（1993-），女，硕士研究生，研究方向为植物生理生态学。E-mail：2365580744@qq.com.责任作者：郑云普（1981-），男，博士，副教授，研究方向为全球变化生态学及植物生理生态学。E-mail：zhengyunpu_000@sina.com.基金项目：国家自然科学基金青年资助项目(31400418)；河北省自然科学基金面上资助项目(C2016402088)；河北省高等学校青年拔尖人才计划资助项目(BJ2016012)；中国博士后科学基金面上资助项目(2014M561044）；中国博士后科学基金特别资助项目（2016T90128)；邯郸市科技计划资助项目(1622201052)；邯郸学院校级科研资助项目(15217)。