GU Xin,CHEN Tianxiang,SUN Quan.Alleviating Effects of Different Measures on Phenolic Acids Toxicity in Cucumber Seedling Under Shed Protection[J].Northern Horticulture,2017,41(08):38-44.[doi:10.11937/bfyy.201708010]
不同措施对设施黄瓜幼苗酚酸毒害的缓解效应
- Title:
- Alleviating Effects of Different Measures on Phenolic Acids Toxicity in Cucumber Seedling Under Shed Protection
- Keywords:
- cucumber; phenolic acid; fertilization; alleviation
- 文献标志码:
- A
- 摘要:
- 以“津春5号”黄瓜为试材，采用盆栽方法，在石英砂中添加外源苯丙烯酸和对羟基苯甲酸（100 mg?kg-1），分别施用或配施碳化玉米芯、生物有机肥及菌剂，研究了不同外源酚酸物质对植株生理生化指标的影响，及不同施肥措施对其影响的缓解作用。结果表明：黄瓜幼苗对不同外源酚酸物质的毒害反应具有差异，苯丙烯酸对植株主根长的抑制作用较对羟基苯甲酸强，对羟基苯甲酸在根系活力、株高、根冠比和地下部鲜质量指标上具有更强的抑制作用。不同施肥措施对酚酸毒害作用的缓解效应不同。当添加外源苯丙烯酸，碳化玉米芯+生物有机肥有利于黄瓜幼苗丙二醛（MDA）含量减少，茎粗和主根长增加，细胞膜相对透性保持适中水平；单施碳化玉米芯有利于植株根系活力、超氧化物歧化酶（SOD）活性和株高增加，碳化玉米芯+生物有机肥+菌剂有利于根体积、地下部鲜质量和根冠比增加；生物有机肥有利于植株过氧化物酶（POD）活性和地上部鲜质量增加。添加外源对羟基苯甲酸，碳化玉米芯+生物有机肥有利于植株叶绿素含量、SOD活性、POD活性、地下部鲜质量和根冠比增加，MDA含量减少；生物有机肥有利于株高、茎粗和主根长增加；碳化玉米芯+生物有机肥+菌剂有利于根系活力和根体积增加。该试验说明在该试验酚酸浓度范围内，应用适宜的缓解措施，有利于缓解土壤中酚酸物质的毒害作用。
- Abstract:
- ‘JinchunⅤ’cucumber was used as test material.A pot experiment was conducted with cucumber seedling growing under plastic greenhouse.The applied phenolic acids were cinnamic acid and p-hydroxy benzoic acid.Absolute control used neither fertilizer nor phenolic acid(CK).Relative comparison used different phenolic acid singly(100 mg?kg-1 soil)(T0).Four fertilizer treatments were carbonized maize cob(T1)，biological organic fertilizer(T2)，carbonized maize cob cooperated with biological organic fertilizer(T3)，carbonized maize cob cooperated with biological organic fertilizer and microbial fertilizer(T4).The results showed that the reactions of seedlings had discrepancy in poison of two phenolic acids，and the alleviating effects of different fertilizers had distinction in it.The suppressive effect of cinnamic acid on main root length was more than p-hydroxybenzoic acid，but p-hydroxybenzoic acid had significant inhibitory effects on the root activity，plant height，root shoot ratio and underground fresh weight compared with cinnamic acid.In the exogenous cinnamic acid group，the content of MDA reduced in leaves，stem diameter and main root length increased and relative permeability of cell membranes kept proper level in T3.The root activity，SOD activity and plant height increased in T1.The Root volume，underground fresh weight and root shoot ratio increased in T4.The POD activity and aboveground fresh weight increased in T2.In the exogenous p-hydroxy benzoic acid group，the content of MDA reduced in leaves，chlorophyll content，SOD activity，POD activity，underground fresh weight and root shoot ratio increased in T3.The plant height，stem diameter and main root length increased in T2.The root activity and volume increased in T4.In this experiment，the application of appropriate mitigation measures could reduce the toxicity of phenolic acid in the soil.
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备注/Memo
第一作者简介：顾欣（1973-），女,硕士，副教授，现主要从事农业微生物资源与利用的教学与研究等工作。E-mail：guxin929@163.com.基金项目：宁夏重点研发计划资助项目（2015BP02，2017CP03）；宁夏农业科技支撑资助项目（2014NCN06）。