[1]王志超,马秋敏,叶彦雄,等.番茄II型metacaspase蛋白SlMC7抗体效价检测及其在果实中表达特性分析[J].北方园艺,2017,41(16):50-55.[doi:10.11937/bfyy.20170730]
　WANG Zhichao,MA Qiumin,YE Yanxiong,et al.Antibody Titer Detection and Expression Characteristic Analysis in Fruit of Type II Tomato Metacaspase SlMC7 Protein[J].Northern Horticulture,2017,41(16):50-55.[doi:10.11937/bfyy.20170730]

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番茄II型metacaspase蛋白SlMC7抗体效价检测及其在果实中表达特性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷期数: 2017年16 页码: 50-55 栏目: 出版日期: 2017-08-30

Title:: Antibody Titer Detection and Expression Characteristic Analysis in Fruit of Type II Tomato Metacaspase SlMC7 Protein

作者:: 王志超1; 2; 马秋敏2; 叶彦雄2; 温帅2; 曹景顺2; 曲桂芹2; 1.河南省食品药品审评查验中心，河南郑州 450046；2.中国农业大学食品科学与营养工程学院，北京 100083

Author(s):: WANG Zhichao1; 2; MA Qiumin2; YE Yanxiong2; WEN Shuai2; CAO Jingshun2; QU Guiqin2; 1.Henan Provincial Food and Drug Evaluation and Inspection Center，Zhengzhou，Henan 450046；2.College of Food Science and Nutritional Engineering，China Agricultural University，Beijing 100083

关键词:: metacaspase蛋白; 抗体; 蛋白表达; 番茄

Keywords:: metacaspase protein; antibody; protein expression; tomato

DOI:: 10.11937/bfyy.20170730

文献标志码:: A

摘要:: 以原核表达的番茄SlMC7重组蛋白为抗原，制备SlMC7多克隆抗体，采用ELISA和蛋白质点杂的方法，研究了番茄II型metacaspase蛋白(SlMC7)的抗体效价和表达特性，并对SlMC7蛋白及基因在番茄果实发育过程中的表达差异进行了研究，以期为后续的SlMC7在番茄果实中功能性研究提供材料基础和参考依据。结果表明：SlMC7多克隆抗体效价在256 000以上，在1∶10 000的稀释度下，可识别低至1.6 ng的纯蛋白。对各时期番茄果实Western-blot 和Real time PCR结果表明，相对于绿熟期，SlMC7蛋白及基因在破色期果实中表达量显著上升，表明SlMC7与番茄果实成熟存在一定相关性。

Abstract:: SlMC7 prokaryotic expression recombinant protein was used as test materials.SlMC7 polyclonal antiserum was obtained from immunized mice by using purified SlMC7 recombinant protein from Escherichia coli.ELISA and protein dot plot methods were used to detect the antibody titer and expression characteristics and the expression difference at different stages，providing material foundation and reference basis for subsequent SlMC7 functional research in tomato fruits.The results showed that the titer of SlMC7 polyclonal antibodies was more than 1∶256 000 and sensitivity of SlMC7 polyclonal antibodies was 1.6 ng at the dilution of 1∶10 000.SlMC7 protein was detected at different stages，SlMC7 had a higher expression in breaker fruits.SlMC7 might be relevant to the fruit ripening of tomato.

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备注/Memo

第一作者简介：王志超(1991-)，男，硕士研究生，研究方向为植物生理。E-mail：wangzhichao101@126.com.

责任作者：曲桂芹(1970-)，女，博士，副教授，研究方向为果蔬成熟衰老生理及转基因植物功能分析。E-mail：quguiqin2000@sina.com.

基金项目：国家自然科学基金资助项目(31672207)。

收稿日期：2017-04-05

更新日期/Last Update: 2017-08-29