SUN Huizhong,SONG Yueqin,WANG Xiaodong,et al.Potassium Releasing Activity and Identification of LJP21 From Rhizosphere Soil of Paeonia ostii ‘Fengdan’[J].Northern Horticulture,2017,41(07):136-139.[doi:10.11937/bfyy.201707028]
“凤丹”牡丹根际土壤LJP21菌株的解钾活性及其鉴定
- Title:
- Potassium Releasing Activity and Identification of LJP21 From Rhizosphere Soil of Paeonia ostii ‘Fengdan’
- Keywords:
- Paeonia ostii‘Fengdan’; potassium bacteria; isolation; identification
- 文献标志码:
- A
- 摘要:
- 以“凤丹”牡丹根际土壤为试材，采用稀释平板法，分离根际环境中的解钾菌，并进行活性评估及鉴定。结果表明：共分离到了4株解钾菌（LJP3、LJP7、LJP21、LJP23）,在28 ℃、180 r?min-1条件下发酵培养7 d后，LJP21菌株的发酵液中水溶性速效钾含量最高，达到2.6 μg?mL-1，将其作为鉴定菌株。通过表型特征观察、生化特性测定与16S rDNA测序的多相分析，初步将菌株LJP21归为微小杆菌属Exiguobacterium，暂命名为Exiguobacterium sp.LJP21。菌株LJP21可作为菌肥或生物钾肥开发的备用出发菌株。
- Abstract:
- Rhizosphere soil of Paeonia ostii ‘Fengdan’ was used as test material.The isolation，activity evaluation and identification of potassium bacteria in the rhizosphere environment of Paeonia ostii ‘Fengdan’ were studied by using dilution plate method.The results showed that four strains LJP3，LJP7，LJP21，LJP23 of potassium bacteria were isolated.After 7 days fermentation under the condition of 28 ℃，180 r?min-1，the potassium content in fermentation liquid of LJP21 reached a maximum of 2.6 μg?mL-1.By observing the phenotypic characteristics，biochemical characteristics and 16S rDNA analysis，strain LJP21 was determined to belonged to the genus Exiguobacterium，and tentatively named Exiguobacterium sp.LJP21.Strain LJP21 could be used as a reserve strain in fertilizer or biofertilizer development.
参考文献/References:
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备注/Memo
第一作者简介：孙会忠（1976-），男，博士，副教授，现主要从事生物资源学教学与研究等工作。E-mail：huizhong66@163.com.责任作者：侯小改（1966-），女，博士，教授，现主要从事植物学教学与研究等工作。E-mail：lyxc2018@sina.com.基金项目：河南省高等学校重点科研资助项目（16A180003）；河南省烟草公司科学研究与技术资助项目（HYKJ201302）。